| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,899,621 | G→A | V399I (GTT→ATT) | pdeD → | putative c‑di‑GMP phosphodiesterase PdeD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,899,621 | 0 | G | A | 97.8% | 146.0 / NA | 46 | V399I (GTT→ATT) | pdeD | putative c‑di‑GMP phosphodiesterase PdeD |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (26/19); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCATGTTGGCATTAACGTCGCACCCAGCCATTTTCGCCGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATGTTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACATTACGTCCTGACGTGCTGAAAATTGATAAGTCA > NC_000913/1899484‑1899752 | ttCATGTTGGCATTAACGTCGCACCCAGCCATTTTCGCCGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATAtt > 2:886894/1‑140 (MQ=255) gCATTAACGTCGCACCCAGCCATTTTCGCCGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCgg > 1:923898/1‑140 (MQ=255) cGCACCCAGCCATTTTCGCCGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCcgcg > 1:242150/1‑140 (MQ=255) gCACCCAGCCATTTTCGCCGTGATGTGCTTATAAAAGGTCTCAATCAGTACTGG‑TTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAg < 1:442521/140‑1 (MQ=255) cACCCAGCCATTTTCGCCGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAg < 1:886894/140‑1 (MQ=255) cACCCAGCCATTTTCGCCGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAg < 1:782816/140‑1 (MQ=255) gCCATTTTCGCCGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCAt > 1:990633/1‑140 (MQ=255) gCCGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAAc > 1:563569/1‑140 (MQ=255) cGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCTGCGAGCTGCATCGTAAAAACGt > 2:670784/1‑140 (MQ=255) tggtgTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCa > 2:228363/1‑140 (MQ=255) tgCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTg > 2:58212/1‑140 (MQ=255) gCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTgg < 1:540419/140‑1 (MQ=255) cTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGc < 2:519429/140‑1 (MQ=255) aTAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGAt > 2:577206/1‑140 (MQ=255) aGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATtgatg > 2:8965/1‑140 (MQ=255) tcAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTc > 2:805411/1‑140 (MQ=255) tcAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTc > 1:41601/1‑140 (MQ=255) tcAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTc > 2:636690/1‑140 (MQ=255) aaTCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCgg > 2:549252/1‑140 (MQ=255) cAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCTGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCAc < 1:670784/140‑1 (MQ=255) tACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCgg > 2:845721/1‑140 (MQ=255) aCTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGc < 1:577206/140‑1 (MQ=255) tttAGCGCTCACCCGACCCAGCTACTGATCCACAAAATCTCCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAg < 2:447331/140‑1 (MQ=255) gcgcTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGGTTGTTGACTTCGGGACCGGCAACAGCTCg > 1:447331/1‑140 (MQ=255) gcgcTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCAcc > 2:919300/1‑128 (MQ=255) gcgcTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCAcc < 1:919300/128‑1 (MQ=255) gcgcTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCg > 2:821007/1‑140 (MQ=255) gcgcTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCg > 2:236305/1‑140 (MQ=255) gcgcTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCg > 1:26700/1‑140 (MQ=255) cACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAGCGTCAAACAGGCTATTGATGACTTCGGCACCGGCAACAGCTCGTTTTc > 2:383249/1‑140 (MQ=255) cACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTc > 2:658586/1‑140 (MQ=255) cACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTc > 2:999902/1‑140 (MQ=255) aTTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCt < 2:357944/140‑1 (MQ=255) aTTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCt > 2:740835/1‑140 (MQ=255) aGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGaa < 1:58212/140‑1 (MQ=255) cTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCGCCGGCAACAGCTCGTTTTCCTGGCTTGAAACAtt < 1:979981/140‑1 (MQ=255) cTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACAtt < 1:805411/140‑1 (MQ=255) cTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACAtt < 1:636690/140‑1 (MQ=255) aaaTCACCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACATTACGTCCTGAc < 2:242150/140‑1 (MQ=255) cACCGAACGCGATGCCTTACTGCATGTTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACATTACGTCCTGACGTGc < 1:383249/140‑1 (MQ=255) aCCGAACGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACATTACGTCCTGACGTGCt > 2:84365/1‑140 (MQ=255) ccGAACGCGATGCCTTACTGGACATTGATTATCGGATTGCCCGCGCGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTTAAACATTACGTCATGACGTGCTg > 2:870621/1‑140 (MQ=255) cGAACGCGATGCGTTACTGGATGTTGATTATGGGACTGCCCGCGAGCTCCATCGTAAAAACGTCAAGCTTGCGATTGATAACTTCCGCACCGGCAACAGCTCGGTTTCCTGGCTTGAAACATTACGTCCTGACGTGCTGa < 2:428095/140‑1 (MQ=255) aCGCGATGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACATTACGTCCTGACGTGCTGaaaa < 1:8965/140‑1 (MQ=255) aTGCCTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACATTACGTCCTGACGTGCTGAAAATTGAt < 1:821007/140‑1 (MQ=255) ccTTACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACATTACGTCCTGACGTGCTGAAAATTGATAAg < 2:984722/140‑1 (MQ=255) tACTGGATATTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACATTACGTCCTGACGTGCTGAAAATTGATAAGTCa < 2:645835/140‑1 (MQ=255) | TTCATGTTGGCATTAACGTCGCACCCAGCCATTTTCGCCGTGGTGTGCTGATAAAAGATCTCAATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATGTTGATTATCGGATTGCCCGCGAGCTGCATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGCTCGTTTTCCTGGCTTGAAACATTACGTCCTGACGTGCTGAAAATTGATAAGTCA > NC_000913/1899484‑1899752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |