Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,928,727	T→A	D38V (GAC→GTC)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,727	0	T	A	100.0%	161.5 / NA	50	D38V (GAC→GTC)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/27);  total (23/27)

TAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAAC  >  NC_000913/1928592‑1928863                                                                                                                                        |                                                                                                                                         tAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAg                                                                                                                                      >  2:1004073/1‑140 (MQ=255)         tttGATGTAGGGCGCAGAAACTTCTCGTTGCGGACTGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTg                                                                                                                              >  2:86997/1‑140 (MQ=255)               gTAGGGCGCAGAAACGTCTCATAGCAGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTgag                                                                                                                        <  1:473047/140‑1 (MQ=255)                   ggCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAAcg                                                                                                                    >  2:850947/1‑140 (MQ=255)                   ggCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTCCTTGTTGTAGGAAGACCAGGACTTCTGGCTGAGAAcg                                                                                                                    >  1:415749/1‑140 (MQ=255)                     cgcAGAAACTTCTCGTTGCGGAATGCGGTCGGTGATTTCCTTTAAGATGGGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAAcgcg                                                                                                                  <  1:913620/140‑1 (MQ=255)                     cgcAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAAcgcg                                                                                                                  >  2:1016092/1‑140 (MQ=255)                               tctcGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGAGCAGGACTTCTGGCTGAGAACGCGTATCGTCCCg                                                                                                        >  2:470252/1‑140 (MQ=255)                                 tcGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCa                                                                                                      <  1:1016092/140‑1 (MQ=255)                                 tcGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCa                                                                                                      <  1:57177/140‑1 (MQ=255)                                   gTTGCGGAATGCGGTTGATGATTTCCTTTTAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGc                                                                                                    <  1:450768/140‑1 (MQ=255)                                      gCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCag                                                                                                 <  2:953773/140‑1 (MQ=255)                                      gCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCag                                                                                                 <  1:789369/140‑1 (MQ=255)                                      gCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCag                                                                                                 >  2:186389/1‑140 (MQ=255)                                            tGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTaa                                                                                           >  2:253690/1‑140 (MQ=255)                                                gTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCa                                                                                                      >  2:840757/1‑125 (MQ=255)                                                gTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCa                                                                                                      <  1:840757/125‑1 (MQ=255)                                                    atgatTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGAt                                                                                   >  1:983460/1‑140 (MQ=255)                                                           ccTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTa                                                                            >  1:53793/1‑140 (MQ=255)                                                                      cGATCCTGCAAGGCTTGTTGTGAGCCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAg                                                                 >  2:96962/1‑140 (MQ=255)                                                                          ccTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTc                                                             <  1:1005617/140‑1 (MQ=255)                                                                          ccTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTc                                                             <  1:139570/140‑1 (MQ=255)                                                                          ccTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTc                                                             <  2:779832/140‑1 (MQ=255)                                                                             gCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATg                                                          >  1:821408/1‑140 (MQ=255)                                                                                  gCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTg                                                     <  1:1004073/140‑1 (MQ=255)                                                                                   cTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTgg                                                    >  2:506663/1‑140 (MQ=255)                                                                                     tgttgtGAGGCCATCGCCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTgggg                                                  >  1:620171/1‑140 (MQ=255)                                                                                      gttgtGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGa                                                 >  2:943895/1‑140 (MQ=255)                                                                                            aGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgg                                           >  2:207827/1‑140 (MQ=255)                                                                                             ggCCATCACCCGATGCCCGTAATTATTTTCTTGTTGCTGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcggg                                          <  1:489981/140‑1 (MQ=255)                                                                                               ccATCACCCGATGACTGTAACTCTTATCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggcg                                        <  1:482885/140‑1 (MQ=255)                                                                                                       cGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGTCGGCTTTTgg                                <  2:715189/140‑1 (MQ=255)                                                                                                       cGATGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTgg                                <  2:639049/140‑1 (MQ=255)                                                                                                         aTGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                              <  1:729309/140‑1 (MQ=255)                                                                                                         aTGACCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                              <  2:887474/140‑1 (MQ=255)                                                                                                            aCCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCa                           <  2:620171/140‑1 (MQ=255)                                                                                                            aCCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCa                           <  1:506663/140‑1 (MQ=255)                                                                                                            aCCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCa                           <  2:821408/140‑1 (MQ=255)                                                                                                            aCCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCa                           <  2:828542/140‑1 (MQ=255)                                                                                                            aCCGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCa                           <  2:1020593/140‑1 (MQ=255)                                                                                                              cGTAACTATTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATg                         >  1:612278/1‑140 (MQ=255)                                                                                                                    tATTTTCTTGTTGCATGTAGACTAGTACTCCTGGCTGTGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTg                   <  1:470252/140‑1 (MQ=255)                                                                                                                     aTTTTCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAGGCGTCATGACGTCGGGGATGCGGGCGGCTTATGGTAGCATGTTATTGt                  >  2:373424/1‑140 (MQ=255)                                                                                                                        ttCTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCTTCATGGCGTGGGAAATCCGGGCGGCTTTTGCTAGCATGTTATTGttct               >  2:404986/1‑140 (MQ=255)                                                                                                                          cTTGTTGCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttcttt             >  1:619402/1‑140 (MQ=255)                                                                                                                              gacagggTAGACCAGGACTTCTTGCTGAGAACGCGTACCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTgg         <  1:372124/135‑1 (MQ=255)                                                                                                                                gCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGtt       >  2:856218/1‑140 (MQ=255)                                                                                                                                gCAGGTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGtt       >  1:709701/1‑140 (MQ=255)                                                                                                                                    gTAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaaa   <  1:86997/140‑1 (MQ=255)                                                                                                                                     tAGACCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAAc  <  2:553691/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TAGCCATTTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAAC  >  NC_000913/1928592‑1928863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑