| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,934,624 | T→C | intergenic (‑20/+215) | edd ← / ← zwf | phosphogluconate dehydratase/NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,934,624 | 0 | T | C | 96.5% | 194.6 / ‑2.7 | 58 | intergenic (‑20/+215) | edd/zwf | phosphogluconate dehydratase/NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (26/30); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACG > NC_000913/1934488‑1934755 | cTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGt < 2:595662/140‑1 (MQ=255) tGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTc < 1:693786/140‑1 (MQ=255) cGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCTGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCag > 1:11911/1‑140 (MQ=255) aaCGATGAACGGTCGAAGTTTTCGCTTGTTCTCTCCGGGCGAGATAAGCAGAGCGACTCTCCCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCagag < 2:627019/140‑1 (MQ=255) aaCGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTTTGGATTCGTAATGGATCCTGAGATTGAGCTGTCagag > 1:268919/1‑140 (MQ=255) cGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCa < 1:244805/140‑1 (MQ=255) cGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCa < 2:88783/140‑1 (MQ=255) aTGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAgg < 1:608068/140‑1 (MQ=255) aGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAAc < 2:206399/140‑1 (MQ=255) aGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAAc < 2:913526/140‑1 (MQ=255) ttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAGCGCGt > 1:870713/1‑140 (MQ=255) tGTTCTATCCGGGCGAGCTAAGCAGAGCGAGTCTCTCGCAAACGTTCATTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTttcatt < 2:268919/140‑1 (MQ=255) tGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTttcatt > 1:26622/1‑140 (MQ=255) gCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGGGTTGTCAGGGCAGGATGATTCACAAAGCGTGTCATTCAGAGGATTTAt > 2:395442/1‑140 (MQ=255) aTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTg > 2:850877/1‑140 (MQ=255) cAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACg < 2:563531/140‑1 (MQ=255) gCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTg > 2:98203/1‑140 (MQ=255) gCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTg > 1:597981/1‑140 (MQ=255) cGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGGTTCATTCAGAGGATTTCTGACTGAAACGCCTGt > 2:479428/1‑140 (MQ=255) gTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAAc > 2:645426/1‑140 (MQ=255) gTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAAc > 2:710323/1‑140 (MQ=255) ctcGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCg < 2:1000118/140‑1 (MQ=255) ctcGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCg > 2:112412/1‑140 (MQ=255) gcgcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAAcc > 1:717781/1‑136 (MQ=255) gcgcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAAcc < 2:717781/136‑1 (MQ=255) tGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCaaaa < 1:597081/140‑1 (MQ=255) tCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGt > 1:975482/1‑140 (MQ=255) tCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGt > 2:587211/1‑140 (MQ=255) cGATTTGTTACGCGTAATAATTTTGGATTCAGAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGtt < 2:405575/140‑1 (MQ=255) cGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGtt > 1:645984/1‑140 (MQ=255) tGTTACGCGTAACAATTGTGGATTCCTAACGGCTCCTCAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt < 1:395442/140‑1 (MQ=255) tGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt < 1:850877/140‑1 (MQ=255) tGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt < 1:512090/140‑1 (MQ=255) tGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt < 1:98203/140‑1 (MQ=255) tGTTACGCGTAACAAATGTGGATTCTTAACGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt < 1:315707/140‑1 (MQ=255) tGTTACGCATAACAATTTTGGACTCATAAAGTCACCTGAAATTGAGCTTTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt < 2:496441/140‑1 (MQ=255) ttACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtca < 2:693574/140‑1 (MQ=255) ttACGCGTAACAATTGTGGATTCATAAAGGCCCCTCAAAATGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtca < 2:434421/140‑1 (MQ=255) ttACGCGTAACAATTCTGGATTCATCAAGGCTCCTGAGATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtca < 1:480534/140‑1 (MQ=255) gcgTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAgtgt < 2:975482/140‑1 (MQ=255) cgTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGCAACGCCTGGAACCGGAGGTCATAGGACAAAATCGTTTCAGTCAGTGta > 2:480534/1‑140 (MQ=255) cgTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAGCCGGAGCGCATTGGTCAAAAAAGTTTCAGTCAGTGta > 1:292737/1‑140 (MQ=255) cgTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCACATCGGGCAAAAACGTTTCAGTCAGTGta > 1:356809/1‑140 (MQ=255) ttGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt > 2:516245/1‑125 (MQ=255) ttGTGGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt < 1:516245/125‑1 (MQ=255) tgGATTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAGAACGTTGCAGTCAGTGTAATAAAAAAAGc > 1:470853/1‑140 (MQ=255) aTTCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTc < 2:11911/140‑1 (MQ=255) ttCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGCAGCTCATAGGGCCAAAACGTTTCAGGCAGTGTAATAAAAAAAGCCTCg > 2:452268/1‑140 (MQ=255) tCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAg < 2:613110/134‑1 (MQ=255) tCATAAAGGCTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAg > 1:613110/1‑134 (MQ=255) cTCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATc > 1:637825/1‑140 (MQ=255) tCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGCGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCGCGTGGGAGAATcc > 2:841377/1‑140 (MQ=255) tCCTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATcc > 2:212357/1‑140 (MQ=255) cTGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGGCAGGGTAATAAAGAAAGTCTCGTGGGTGAATCCGc > 2:304036/1‑140 (MQ=255) tGAAATTGAGCTGTCTGAGCAGTATAATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCa < 1:304036/140‑1 (MQ=255) tGAAATTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCa < 2:26622/140‑1 (MQ=255) aaaTTGAGCTGTCAGAGCAGGATGATTCACAGCGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACg < 2:870713/140‑1 (MQ=255) aaaTTGAGCTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACg < 1:112412/140‑1 (MQ=255) | CTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACG > NC_000913/1934488‑1934755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |