Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,996,183	T→C	N217S (AAC→AGC)	sdiA ←	DNA‑binding transcriptional dual regulator SdiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,996,183	0	T	C	100.0%	179.9 / NA	50	N217S (AAC→AGC)	sdiA	DNA‑binding transcriptional dual regulator SdiA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

AACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATC  >  NC_000913/1996048‑1996314                                                                                                                                        |                                                                                                                                    aaCCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGctttt                                                                                                                                 >  2:809195/1‑140 (MQ=255) aaCCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGctttt                                                                                                                                 >  1:376633/1‑140 (MQ=255)    cGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtg                                                                                                                              >  1:849065/1‑140 (MQ=255)      gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCCTTTCtggt                                                                                                                            >  1:181783/1‑140 (MQ=255)       cTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggtg                                                                                                                           >  1:762248/1‑140 (MQ=255)       cTGATAGCGTGCCTTTCAGCCGCTTTTTGCAACTGGCACCCAGGACAGTAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggtg                                                                                                                           <  2:376633/140‑1 (MQ=255)            aGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAAt                                                                                                                      <  2:159644/140‑1 (MQ=255)            aGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAAt                                                                                                                      <  1:809195/140‑1 (MQ=255)              cGTGCCTTTCAGCCGGTTTTTGGATCTGGCACGCACGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTg                                                                                                                    <  2:351324/140‑1 (MQ=255)                tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                  >  1:925138/1‑140 (MQ=255)                tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                  >  1:3806/1‑140 (MQ=255)                 gCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAGTTTATTTCTGCATGCTTTTCTGGTGGAAATTGAcc                                                                                                                 >  2:340678/1‑140 (MQ=255)                    tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                              <  1:30601/140‑1 (MQ=255)                       cAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATtc                                                                                                           >  2:143515/1‑140 (MQ=255)                          ccGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCa                                                                                                        <  2:3806/140‑1 (MQ=255)                          ccGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCCTTTCTGGTGGAAATTGACCGTATTCTCa                                                                                                        <  2:181783/140‑1 (MQ=255)                             gTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCagag                                                                                                     >  2:6269/1‑140 (MQ=255)                                       cTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACaaaa                                                                                           >  2:923772/1‑140 (MQ=255)                                        tGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAat                                                                                          <  1:6269/140‑1 (MQ=255)                                        tGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAat                                                                                          >  2:431191/1‑140 (MQ=255)                                               cAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCt                                                                                   <  2:762248/140‑1 (MQ=255)                                                 ggACAGAAAAGAGATCACATTACGCCAGTAGGGGCCGCGCAACAGGCATCCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTAt                                                                                 <  2:262195/140‑1 (MQ=255)                                                       aaaaGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgc                                                                           <  2:713868/140‑1 (MQ=255)                                                       aaaaGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgc                                                                           <  1:143515/140‑1 (MQ=255)                                                            agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgct                                                                          >  1:141274/1‑136 (MQ=255)                                                            agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgct                                                                          <  2:141274/136‑1 (MQ=255)                                                            agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgct                                                                          <  2:45342/136‑1 (MQ=255)                                                            agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgct                                                                          >  1:45342/1‑136 (MQ=255)                                                            agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAAATGACCGTAGTCTTAGAGATTGACAAAATCATCGCTATCTCTGCTGATg                                                                      >  2:261596/1‑140 (MQ=255)                                                              aTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGtt                                                                    >  2:938819/1‑140 (MQ=255)                                                                             tAGCGGCCGCGTAACTGGCAACATGAGTCTTATTTGGGGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                     <  1:340678/140‑1 (MQ=255)                                                                             tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                     <  2:56202/140‑1 (MQ=255)                                                                             tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                     >  2:947228/1‑140 (MQ=255)                                                                             tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                     >  1:352528/1‑140 (MQ=255)                                                                             tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                     >  1:69680/1‑140 (MQ=255)                                                                                   ccGCGTAACAGGCAACCTGGGTCTTCTTTAGTGCATTTATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTc                                               <  1:261596/140‑1 (MQ=255)                                                                                       gTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTGCCTTCCGCCGTCCACCTCAGaa                                           >  1:328934/1‑140 (MQ=255)                                                                                              gcagccTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCttt                                    >  2:868318/5‑140 (MQ=255)                                                                                                   cTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTcgc                               >  2:136019/1‑140 (MQ=255)                                                                                                    tGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTcgcg                              <  1:255726/140‑1 (MQ=255)                                                                                                    tGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTcgcg                              <  1:938819/140‑1 (MQ=255)                                                                                                     gggTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCgcgc                             >  2:707984/1‑140 (MQ=255)                                                                                                      ggTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCt                            <  2:352528/140‑1 (MQ=255)                                                                                                        tCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCCGATGTTTTACCTTCCGGCGTCCACCACAGACTTTCTTTTTCGCGCTTg                          >  2:407464/1‑140 (MQ=255)                                                                                                            aTTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGa                      >  1:750890/1‑140 (MQ=255)                                                                                                                  tGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTcgc                               <  2:597015/125‑1 (MQ=255)                                                                                                                  tGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTcgc                               >  1:597015/1‑125 (MQ=255)                                                                                                                     aTTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGGAATTCAtct             >  2:847387/1‑140 (MQ=255)                                                                                                                          tttttttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGc        <  2:654500/140‑1 (MQ=255)                                                                                                                            tttttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCTCCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGt      <  2:212980/140‑1 (MQ=255)                                                                                                                            tttttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGt      <  2:925138/140‑1 (MQ=255)                                                                                                                                tCTGCATGCTTTTCTGGTGGAATTTCACCGTATTCTCGGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcatc  <  1:407464/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    AACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATC  >  NC_000913/1996048‑1996314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑