Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	211,877	A→G	M1M (ATG→GTG) †	yaeR →	VOC domain‑containing protein YaeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	211,877	0	A	G	100.0%	114.5 / NA	34	M1M (ATG→GTG) †	yaeR	VOC domain‑containing protein YaeR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/20);  total (14/20)

GTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGAAGTCTATCGCGAAGCGCGCGACTCATGGAA  >  NC_000913/211741‑212010                                                                                                                                         |                                                                                                                                      gTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCCGAGCGGCTTCCGGGCGCGGAACGTGCTGTTAACAAATAAAGGAGACGTTGTGc                                                                                                                                    >  2:554877/1‑140 (MQ=255) gTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGc                                                                                                                                    >  1:652147/1‑140 (MQ=255)    ttGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCCTCCGGGCGTGTAACGGCCTGTTAACAAATAAAGGAGACGTTGTGCTgg                                                                                                                                 >  2:375272/1‑140 (MQ=255)            gATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTaaaa                                                                                                                         <  2:152750/140‑1 (MQ=255)             atatTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAAc                                                                                                                        <  2:652147/140‑1 (MQ=255)               atTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAg                                                                                                                      <  2:26472/140‑1 (MQ=255)               atTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGCAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAg                                                                                                                      <  1:163967/140‑1 (MQ=255)                 tCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGt                                                                                                                    <  1:80983/140‑1 (MQ=255)                   aCGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTc                                                                                                                  <  1:95829/140‑1 (MQ=255)                     ggCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCAc                                                                                                                >  1:692882/1‑140 (MQ=255)                      gcgcGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGGTCAcc                                                                                                               >  1:159473/1‑140 (MQ=255)                       cgcgAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAg                                                                                                                      <  2:993235/132‑1 (MQ=255)                       cgcgAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAg                                                                                                                      >  1:993235/1‑132 (MQ=255)                                       gACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCg                                                                                              <  1:715579/140‑1 (MQ=255)                                        acagCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGa                                                                                             >  1:555712/4‑140 (MQ=255)                                        aCGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGc                                                                                               >  2:552788/1‑138 (MQ=255)                                        aCGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGc                                                                                               <  1:552788/138‑1 (MQ=255)                                                       tACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGa                                                                              >  1:652480/1‑140 (MQ=255)                                                               cTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCt                                                                      <  1:106485/140‑1 (MQ=255)                                                                       ggCGGGATAACTTGCCAGAGCGGCTTCCGGGCGGGTAACGGGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGGTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTg                                                              >  2:195785/1‑140 (MQ=255)                                                                          gggATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTACAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGa                                                           <  2:692882/140‑1 (MQ=255)                                                                                    gCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATACGGTGAGCAAAGATTTCAACTGCGATATTCTTGGt                                                 >  2:449467/1‑140 (MQ=255)                                                                                      cAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATCCGGTGAGCAAAGCTATCTACTGCGATATTCTTGGttt                                               >  1:316109/1‑140 (MQ=255)                                                                                         agCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCAc                                            >  1:716741/1‑140 (MQ=255)                                                                                          gCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACg                                           <  1:740058/140‑1 (MQ=255)                                                                                               ttCCGGGCGAGTAACGTACTGTCAACAAATAAAGGCGACTTTGTGCTGTGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCa                                      <  2:316109/140‑1 (MQ=255)                                                                                                   gggCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGc                                  <  2:981681/140‑1 (MQ=255)                                                                                                      cGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGaa                               <  2:201569/140‑1 (MQ=255)                                                                                                      cGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGaa                               <  1:881645/140‑1 (MQ=255)                                                                                                          tAACGTGCTGTTAACAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGAAGTCt                           >  1:564559/1‑140 (MQ=255)                                                                                                                        cAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGAAGTCTATCGCGAAgcgcgc             <  2:564559/140‑1 (MQ=255)                                                                                                                        cAAATAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGAAGTCTATCGCGAAgcgcgc             <  2:716741/140‑1 (MQ=255)                                                                                                                         aaaTAAAGGAGACGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGAAGTCTATCGCGAAGcgcgcg            >  1:230130/1‑140 (MQ=255)                                                                                                                            tAAAGGAGACGTTGTGCTGGGTTTAAAACAGTTTCACCATATTGCGATAATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGAAGTCTATCGCGAAGCGCGCGACt         <  2:331053/140‑1 (MQ=255)                                                                                                                                   gaCGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGAAGTCTATCGCGAAGCGCGCGACTCATGGaa  <  1:837711/140‑1 (MQ=255)                                                                                                                                   gaCGTTGTGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGAAGTCTATCGCGAAGCGCGCGACTCATGGaa  >  2:8260/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACGCTGCAAAGCGAAGTCTATCGCGAAGCGCGCGACTCATGGAA  >  NC_000913/211741‑212010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑