| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,016,404 | T→C | A179A (GCT→GCC) | fliH → | flagellar biosynthesis protein FliH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,016,404 | 0 | T | C | 97.3% | 117.5 / NA | 37 | A179A (GCT→GCC) | fliH | flagellar biosynthesis protein FliH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (18/18); minor base G (0/1); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGCCAACGGTGGATAACTCGGCACTGATCAAACAGATCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCA > NC_000913/2016269‑2016539 | gACGCCAACGGTGGATAACTCGACACTGATCAAACAGCTCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCt < 1:435608/140‑1 (MQ=255) gTGGATAACTCGGCACTGATCAGACTGATCCAACAGTCGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCa < 1:481403/140‑1 (MQ=255) gTGGATAACTCGGCACTGATCAAACAGATCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCa < 2:800000/140‑1 (MQ=255) aTCAACCAGATCTAACAGTTGATGCAGCAAGAACCGTTATTCAGCTGTAAACCACAGCTGCGCGTGCACCCGGCTGATCTGCAACGTGTGGATGATATGCTCGGCGCGACCTTACGTTCGCATGGCTTGCGCTTGCgggg < 2:632227/140‑1 (MQ=255) aTCAAACAGATCCAACAGTTGTTGCAGCAAGAATCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCCGCGCCACCTTAAGTTTGCATGACTGGCGCTTGCgggg > 1:344484/1‑140 (MQ=255) aTCAAACAGATCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGTTGATATGCACGGCGGCCCCTTAAGTGTGCAAGGCTGGCGCTTGCgggg > 2:492971/1‑140 (MQ=255) aTCAAACAGATCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCgggg > 1:812685/1‑140 (MQ=255) aTCAAACAGATCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGAGGAGATGCTCGGCGCCATCTTAAGGTTGCATGGCTGGCTCTTGCgggg > 1:632227/1‑140 (MQ=255) aTCAAACAGATCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGGGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCgggg > 1:242338/1‑140 (MQ=255) cAAACAGATCCAACATTTGTTGCAGCAAGCACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCg < 1:492971/140‑1 (MQ=255) aCAGATCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGG‑‑AACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCgggg > 1:544846/1‑133 (MQ=255) aCAGATCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGG‑‑AACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCgggg < 2:544846/133‑1 (MQ=255) tCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTTGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCAcc < 1:117923/140‑1 (MQ=255) tCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTGGGCGCCACTTTAGGATAACATGGCTTGCGCTAGCGGGGCGATCCCAcc > 2:489133/1‑140 (MQ=255) tCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCAcc > 2:845931/1‑140 (MQ=255) cAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAATTTTTCATCGCTGGCGCTTGCGGGGCGATCCCACCCTCCa > 2:307416/1‑140 (MQ=255) tgttgCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCt > 1:197334/1‑140 (MQ=255) ttgCAGCAAGAACCGGTATTCAGCGATAAGCCACAGCCGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTgg < 1:489133/140‑1 (MQ=255) gcagcaAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCTTCCATCCTggcg < 1:935531/140‑1 (MQ=255) cGGTAAACCACAGCTACGCGTGTACGCGGATGATCTGAAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATg < 1:468473/140‑1 (MQ=255) tAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGAT‑CCACCATCCATCCGGGCAGCGGTAAAGGCTCCGCCGATGAAgg > 1:286667/1‑140 (MQ=255) acaGCTGCGCGTGCACCTGGATGATCTGCAACGTGCGGACGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGAT‑CCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGAtct < 2:286667/140‑1 (MQ=255) caGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGAtct < 2:918607/140‑1 (MQ=255) cgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCTATCCTGGCGGCTATAAAGTCTCCGCAGATGAAGGCGATCTCGACGcaa > 2:369031/1‑138 (MQ=255) ggATGATCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCa > 2:545100/1‑140 (MQ=255) gatCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCg < 1:845931/140‑1 (MQ=255) gatCTGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCACGACGCCAGTGTCGCCACTCg < 1:26345/140‑1 (MQ=255) tGCAACGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTgg > 2:847342/1‑140 (MQ=255) aCGTGTGGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCCCCGCCGATGAAGCCGAGCTCGACGCAAGTGTCGCCACTCGCTGGCAAg > 2:435877/1‑140 (MQ=255) tgtgGATGATATGCTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAAc > 1:214217/1‑140 (MQ=255) gatgatATGCTCGGCCCCACCTTAACTTTGCATGGCTTGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTg < 1:435877/140‑1 (MQ=255) atgatATGCTCGGTGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGc > 1:840633/1‑140 (MQ=255) gCTCGGTGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTgg < 2:840633/140‑1 (MQ=255) cTCGGCGCCACCTTACGTTTGCGTGACTGTCGCTTGCTGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc < 1:483237/140‑1 (MQ=255) cTCGGCGCCACCTTAAGTTTGCTTGTCTGCCGCTTGCTGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc < 1:304191/140‑1 (MQ=255) cTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc < 2:214217/140‑1 (MQ=255) cTCGGCGCCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc < 1:554805/140‑1 (MQ=255) gcgcCACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGGCGCCACTGGCTGGCGAGAACTATGCCGTCTGgcagca > 1:327342/1‑140 (MQ=255) | GACGCCAACGGTGGATAACTCGGCACTGATCAAACAGATCCAACAGTTGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCA > NC_000913/2016269‑2016539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |