Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,031,164 C→G G385A (GGT→GCT)  dcm ← DNA‑cytosine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,031,1640CG96.9% 108.3 / ‑3.0 33G385A (GGT→GCT) dcmDNA‑cytosine methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (16/15);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

GGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACC  >  NC_000913/2031028‑2031291
                                                                                                                                        |                                                                                                                               
ggCCTGAGTGTCCGAATCCGGATTACGGAATATAGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCg                                                                                                                              <  2:445341/140‑1 (MQ=255)
    tGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGc                                                                                                                          >  2:763404/1‑140 (MQ=255)
                  cGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCcgc                                                                                                            >  2:6344/1‑140 (MQ=255)
                    gAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCATCATAGTATTTCTCAGCCGTGCCCATATCCCAGCCGcga                                                                                                          >  2:336481/1‑140 (MQ=255)
                                   cTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTcc                                                                                           >  2:835473/1‑140 (MQ=255)
                                   cTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTcc                                                                                           >  2:246699/1‑140 (MQ=255)
                                          cGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCAt                                                                                    <  1:925008/140‑1 (MQ=255)
                                                   aaaaCCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                           <  2:223896/140‑1 (MQ=255)
                                                     aaCCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaataa                                                                         >  2:563673/1‑140 (MQ=255)
                                                         cATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAAcgcg                                                                     <  1:763404/140‑1 (MQ=255)
                                                          aTTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                           <  1:6115/133‑1 (MQ=255)
                                                          aTTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGtaat                                                                           >  2:6115/1‑133 (MQ=255)
                                                          aTTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACgcgc                                                                    >  1:1013785/1‑140 (MQ=255)
                                                             aaGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGa                                                                 <  1:835473/140‑1 (MQ=255)
                                                              aGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGAc                                                                <  1:844550/140‑1 (MQ=255)
                                                                  cgcgcATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACTCGCAGACAGCg                                                            <  1:520323/140‑1 (MQ=255)
                                                                  cgcgcATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAAATTCTGCGCCATCTTTGTAATAACGCGCAGACAGCg                                                            >  2:366466/1‑140 (MQ=255)
                                                                          cccGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTg                                                    <  1:846260/140‑1 (MQ=255)
                                                                                 cGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTCCCGCGCCAGCTTTGGAGTAACGCGCAGACAGCGTACGCGCGACGCttt                                             >  2:456397/1‑140 (MQ=255)
                                                                                     aaCCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCgg                                         >  2:926564/1‑140 (MQ=255)
                                                                                          aCGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGt                                    >  1:810611/1‑140 (MQ=255)
                                                                                                 cGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATa                             <  2:810611/140‑1 (MQ=255)
                                                                                                 cGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATa                             <  1:246699/140‑1 (MQ=255)
                                                                                                     gTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATATCGCGCAGAAAGCCTACACGTGACGCTTTGCGGATTGTTCGGATAAAcc                         >  2:390180/1‑140 (MQ=255)
                                                                                                      ttGCTGATTCAGCGGGTCGTCACAGACTTTCTCAGCCATGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCa                        <  1:456397/140‑1 (MQ=255)
                                                                                                       tGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGt                                    <  2:698341/127‑1 (MQ=255)
                                                                                                       tGCTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGt                                    >  1:698341/1‑127 (MQ=255)
                                                                                                         cTGATTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTc                     >  2:715386/1‑140 (MQ=255)
                                                                                                            aTTCAGCGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCAt                  <  2:1013785/140‑1 (MQ=255)
                                                                                                              tCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATaa                >  2:210223/1‑140 (MQ=255)
                                                                                                                  cGGATCGTCAAAGTCTTTCTCATCCTTGGCCATATCCAAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGa            <  1:336481/140‑1 (MQ=255)
                                                                                                                  cGGATCGTCAAAGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGa            <  1:810453/140‑1 (MQ=255)
                                                                                                                            aaGTCTTTCTCAGCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTAcc  >  1:550081/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                               
GGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAATTTCCGCGCCATCTTTGTAATAACGCGCAGACAGCGTACGCGTGACGCTTTGCGGATTGTTCGGATAAACCATTCCATAACCGAAGCCGTTACC  >  NC_000913/2031028‑2031291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑