| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,072,948 | T→C | D470D (GAT→GAC) | flu → | CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,072,948 | 0 | T | C | 94.4% | 55.4 / NA | 18 | D470D (GAT→GAC) | flu | CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (6/11); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCACGGATACCACGGTAAATGGCGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAAT > NC_000913/2072829‑2073083 | gCCACGGATACCACGCTAAATGTCGGACTTTTCACCGTCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAgg < 1:464721/140‑1 (MQ=255)agacaggAAACCACGGGAAATGGCGGACTGATCTCGGCCGGGGGCGGCTCACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGAGGGTGAACAACGACACCCTGACCATCCGTGAAgg < 2:484345/135‑1 (MQ=255) aTACCACGGTAAATGGCGGACTATTCACCGCCACGAGCGCCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCa < 2:448126/140‑1 (MQ=255) aTACCACGATAAATGGCTGACTGTTCACCGCCAGGAGCGTCACACTGGTGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCa < 2:451424/140‑1 (MQ=255) ggTAAATGGCGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGc > 1:831495/1‑140 (MQ=255) aaaTGGCGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGAGGGTGAACAACGAGACCCTGACCATCCGGGAAGGCGATGCACTccctgtct > 1:484345/1‑134 (MQ=255) aTGGCGGACTGTTCACCTCCAGCGGCGGCACCCTGGCGGTCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGa < 2:430598/140‑1 (MQ=255) gACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGctct < 2:219051/140‑1 (MQ=255) aCTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGGGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCTGC‑GGGAgggcccct > 1:1150/1‑134 (MQ=255) cGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGGGAACAACGACACCCTGACCATCCGTGAAGTCAAACCACTCCTACAGGAAGGCTCTCTCACCGGTa > 2:500111/1‑140 (MQ=255) ggggCGGCACACTGGCGGGCACCCCCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGGGAACAACGACACCCTGACCCTCCGTGAAGGCCATGCACTCCTGCAGGGAGGCCCTCTCACCGGTAACGGc > 2:215654/1‑140 (MQ=255) cacTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGaa < 2:831495/140‑1 (MQ=255) accacGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTgg < 2:80944/140‑1 (MQ=255) gagacagtACTTACCCTTTCCGCGAAGACGGAGAAGAACAACACCCTGGCCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAg < 2:407546/133‑1 (MQ=255) ccATACTTACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAac > 1:680917/1‑140 (MQ=255) tttACCCTTTCCGGGAAGACGGGGAACACCGATCCCCTAACCCTCCGTGAGGGCGAGCCCCTCCTGC‑GGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACcaca < 2:1150/139‑1 (MQ=255) ttACCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACcaca < 2:702368/140‑1 (MQ=255) aCCCTTTCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAAACAGGAAGGGGCACATTCACTGTCAGCAACACCACACt > 1:285582/1‑140 (MQ=255) ttCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCAccc > 2:928904/1‑140 (MQ=255) tCCGGGAAGACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGACGTGGCACACTCTCTATCAGCACCACCacacctgtctc > 1:407546/1‑133 (MQ=255) gACGGTGAACAACGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCg < 2:209483/140‑1 (MQ=255) acGACACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCtgaat > 2:716405/1‑140 (MQ=255) | GCCACGGATACCACGGTAAATGGCGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAAT > NC_000913/2072829‑2073083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |