Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,133,799	T→C	E618E (GAA→GAG)	wzc ←	protein‑tyrosine kinase Wzc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,133,799	0	T	C	100.0%	175.4 / NA	52	E618E (GAA→GAG)	wzc	protein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/27);  total (25/27)

GTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCC  >  NC_000913/2133671‑2133931                                                                                                                                 |                                                                                                                                     gTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGAtt                                                                                                                           >  1:1012423/1‑140 (MQ=255) gTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAACGTGCGCTCATCAACAGCTCAGAAGGAtt                                                                                                                           >  1:332507/1‑140 (MQ=255)    ccGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTgg                                                                                                                        >  2:23154/1‑140 (MQ=255)       aCATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTggcgg                                                                                                                     >  1:98998/1‑140 (MQ=255)            acgacCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCt                                                                                                                >  1:166151/1‑140 (MQ=255)               acCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGAc                                                                                                             <  1:729486/140‑1 (MQ=255)               acCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGAc                                                                                                             <  1:531600/140‑1 (MQ=255)               acCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGAc                                                                                                             <  1:933441/140‑1 (MQ=255)               acCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGAc                                                                                                             <  1:104249/140‑1 (MQ=255)                  aacaaTTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTAATCAACAGCTCAGAAGGATTTGGCGGCACCTGACcgc                                                                                                          >  1:404808/1‑140 (MQ=255)                   acaaTTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACcgcg                                                                                                         <  2:166151/140‑1 (MQ=255)                        tGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGa                                                                                                    <  2:559209/140‑1 (MQ=255)                         gCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTTGCTCATCATCAGCTCAAAAGGATTTCGCGGCACCTGACCGCGCGGGAt                                                                                                   >  2:341661/1‑140 (MQ=255)                         gCCGCATCGGTCACTGCAAGAACCGGCGGCGTATCAATCTACACCAGGGCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGAt                                                                                                   <  2:435096/140‑1 (MQ=255)                          ccGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACGTGACCGCGCGGGATc                                                                                                  >  1:492121/1‑140 (MQ=255)                             cATCGGTCACTGCCATAATCGGAGGTGTACCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAgg                                                                                               <  1:265866/140‑1 (MQ=255)                                  gTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCATCAGCTCAGCAGGATTTCGCGGCACCGGACCTCGCGGGATCAGGTCaaa                                                                                          >  2:471825/1‑140 (MQ=255)                                               tCGGCGGCGTATCAATCAACACGAGCTCACAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATagag                                                                             <  1:341661/140‑1 (MQ=255)                                                        tATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTa                                                                    <  2:1012423/140‑1 (MQ=255)                                                           caatcaaCACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTagca                                                                 <  2:967175/140‑1 (MQ=255)                                                            aatcaaCACCAGGTCATCGTTTTTACTCGCCGAGTTCACCAGTTCGGCCAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTagcag                                                                <  2:332507/140‑1 (MQ=255)                                                            aatcaaCACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTagcag                                                                <  1:854181/140‑1 (MQ=255)                                                            aatcaaCAACAGGTCATAGTTTTTACTCGCCTAGTACACTAGTTCGGCCAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTagcag                                                                <  2:498612/140‑1 (MQ=255)                                                             atcaaCACCAGGTCATTATTTTGACTCGCCCTGCTCACCGGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTagcagc                                                               <  2:459914/140‑1 (MQ=255)                                                                  cacCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCTGGTCAAATTTGGCAATAGAGGTCGGTTTagcagc                                                               >  1:216203/1‑135 (MQ=255)                                                                  cacCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCTGGTCAAATTTGGCAATAGAGGTCGGTTTagcagc                                                               <  2:216203/135‑1 (MQ=255)                                                                  cacCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGATCAAATTGGGCGATGGAGGTCAGTTTAGCAGCGgtag                                                          >  1:270657/1‑140 (MQ=255)                                                                     cAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTaa                                                       >  2:201411/1‑140 (MQ=255)                                                                       ggTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAata                                                     >  2:553205/1‑140 (MQ=255)                                                                          cATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTGGGCAATAGAGGACGCTTTAGCAGCGGTAGTAATATCg                                                  >  1:478765/1‑140 (MQ=255)                                                                               tttttACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCACTAGAGGTCGGTCTATCAGCGGTAGTAATATCGCCCTg                                             >  1:473503/1‑140 (MQ=255)                                                                                  ttACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGAcc                                          >  1:222117/1‑140 (MQ=255)                                                                                  ttACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGAcc                                          >  2:686457/1‑140 (MQ=255)                                                                                         cccAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGCCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGa                                   >  2:94575/1‑140 (MQ=255)                                                                                                 aCCAGTTCGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGa                           <  1:201411/140‑1 (MQ=255)                                                                                                   cAGTTCGGCAAAGCGGTCGCTCATCCACAGCTCAGAACGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACa                         <  1:391559/140‑1 (MQ=255)                                                                                                   cAGTTCGGCAAAGCGATCGCTCGTCTACATCTCAGAACGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACa                         <  1:471825/140‑1 (MQ=255)                                                                                                        cGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCa                    >  2:83587/1‑140 (MQ=255)                                                                                                        cGGCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCa                    >  2:83571/1‑140 (MQ=255)                                                                                                          gCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCAtt                  <  2:1001627/140‑1 (MQ=255)                                                                                                          gCAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTCGGCGGCCCCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCACTGACCAATCAGAATTTCCGACAGGCCAtt                  <  1:979237/140‑1 (MQ=255)                                                                                                          gCAAAGCGATCGCTCAAGAACAGCTCAGAATGATTCGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCAtt                  <  2:492121/140‑1 (MQ=255)                                                                                                           cAAAGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTa                 >  2:555779/1‑140 (MQ=255)                                                                                                              aGCGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACg              >  1:829684/1‑140 (MQ=255)                                                                                                                cGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGa                           <  2:544164/125‑1 (MQ=255)                                                                                                                cGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGa                           >  1:544164/1‑125 (MQ=255)                                                                                                                cGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGtt            >  1:198937/1‑140 (MQ=255)                                                                                                                cGTTCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGtt            >  2:687636/1‑140 (MQ=255)                                                                                                                  ttCGCTCATCAACAGCTCAGAATGATTTGGCTGCATCTGTCCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGttat          <  2:404808/140‑1 (MQ=255)                                                                                                                  ttCGCTCATCAACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGttat          <  2:98998/140‑1 (MQ=255)                                                                                                                  ttCGCTCATCAACAGCGTAGAATGATTTGGCGGCACCTGCCCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGttat          <  2:473503/140‑1 (MQ=255)                                                                                                                       tcatcaACAGCTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTg     <  2:222117/140‑1 (MQ=255)                                                                                                                          tcaACAGCTCAGAAGGAATTGGCGGCACCTTCCCGCGCGCGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGccc  <  2:478765/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     GTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCC  >  NC_000913/2133671‑2133931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑