Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,180,672	T→C	V193A (GTT→GCT)	yegU →	putative ADP‑ribosylglycohydrolase YegU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,180,672	0	T	C	100.0%	105.0 / NA	31	V193A (GTT→GCT)	yegU	putative ADP‑ribosylglycohydrolase YegU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/16);  total (15/16)

TGATGTTGATTCCTTTATTGATGATGTAGCGCTGGCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGA  >  NC_000913/2180535‑2180804                                                                                                                                          |                                                                                                                                     tGATGTTGATTCCTTTATTGATGATGTAGCGCTGGCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGAttgctg                                                                                                                                    >  2:988961/1‑140 (MQ=255)      ttGATTCCTTTATTGATGATGTAGCGCTGGCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTcc                                                                                                                               <  1:988961/140‑1 (MQ=255)        gATTCCTTTATTGATGATGTAGAGCTGGCGTTCAGGCCGTCCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCt                                                                                                                             <  2:450523/140‑1 (MQ=255)             cTTTATTGATGATGTAGCGCTGGCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCtt                                                                                                                        >  1:182101/1‑140 (MQ=255)                tATTGATGATGTAGCGCTGGCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGCGAAAGCTGGTCAGCTATTGCTAATTCCCTGCCTTCaa                                                                                                                     >  1:277958/1‑140 (MQ=255)                    gatgatGTAGCGCTGGCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTgc                                                                                                                 <  2:173694/140‑1 (MQ=255)                       gatgTAGCGCTGGCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGcgcg                                                                                                              >  2:868423/1‑140 (MQ=255)                                   gCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATg                                                                                                         >  2:774165/1‑133 (MQ=255)                                   gCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATg                                                                                                         <  1:774165/133‑1 (MQ=255)                                        cAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCCCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAAc                                                                                             <  1:346099/140‑1 (MQ=255)                                           cccGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGGTTGCTGATTCCCAGCCGTCAATTGCGCGACATGCACAGCAAAAACGCa                                                                                          >  2:346099/1‑140 (MQ=255)                                           cccGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCa                                                                                          <  2:49012/140‑1 (MQ=255)                                           cccGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCa                                                                                          >  1:49012/1‑140 (MQ=255)                                           cccGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCa                                                                                          <  1:228793/140‑1 (MQ=255)                                             cGACCCATAAATCCGATCTGGCGGTTCCAGGTGCGGTAGTCATCGCACGGGCGACTTCTCGAGCCATTGACGGAAAAAGCTGGTCAGCGACTTCTGATTCCCCGCCCTCAATTGCGGGACATGCACAGCAAAAACGCATc                                                                                        >  2:714468/1‑140 (MQ=255)                                               aCCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCAc                                                                                      <  1:723334/140‑1 (MQ=255)                                                       atccgatccgGCGGTTCTAGGCTCGGTAGTTCTCGGATGGGCGATTTCGCGTGCCATTGACGGAGAAAGCTGGACAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCa                                                                              <  2:485158/140‑1 (MQ=255)                                                         ccGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAgc                                                                            <  1:764061/140‑1 (MQ=255)                                                              cTGGCGGTTGCAGGCGCGGTAGTCATCGCATTGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTTCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTc                                                                       >  2:801803/1‑140 (MQ=255)                                                              cTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTc                                                                       >  2:750325/1‑140 (MQ=255)                                                                      tGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTggcgg                                                               <  1:776149/140‑1 (MQ=255)                                                                                      aTCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTgc                                               <  1:981718/140‑1 (MQ=255)                                                                                           aTGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGa                                          <  1:714468/140‑1 (MQ=255)                                                                                              ggCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGaaaa                                       >  1:986863/1‑140 (MQ=255)                                                                                              ggCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGaaaa                                       >  1:245623/1‑140 (MQ=255)                                                                                                    ttCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTgc                                 >  1:578520/1‑140 (MQ=255)                                                                                                             cATTGACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCg                        >  1:665374/1‑140 (MQ=255)                                                                                                                 gACGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACgg                    >  1:158763/1‑140 (MQ=255)                                                                                                                   cGGAGAAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCa                  <  2:158763/140‑1 (MQ=255)                                                                                                                      agaAAGCTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCg               >  1:909144/1‑140 (MQ=255)                                                                                                                            cTGGTCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCg         >  1:117470/1‑140 (MQ=255)                                                                                                                                tCAGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAg     <  2:245623/140‑1 (MQ=255)                                                                                                                                  aGCGATTGCTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGTTTGCGCTGCAAAGTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCg   >  2:400190/1‑140 (MQ=255)                                                                                                                                   gCGATTGCTGATTCCCTCCCTTTAATTGCGCCACACGCATAGCAAAAAAGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGa  <  2:277958/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TGATGTTGATTCCTTTATTGATGATGTAGCGCTGGCGTCCAGCCCGACCCATAAATCCGATCTGGCGGTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGA  >  NC_000913/2180535‑2180804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑