Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,193,578	T→C	G197G (GGA→GGG)	mrp ←	P‑loop NTPase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,193,578	0	T	C	100.0%	145.7 / NA	41	G197G (GGA→GGG)	mrp	P‑loop NTPase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/25);  total (16/25)

CACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGTCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCC  >  NC_000913/2193442‑2193704                                                                                                                                         |                                                                                                                               cACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCCCCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCa                                                                                                                             <  2:36126/140‑1 (MQ=255) cACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCa                                                                                                                             >  1:769223/1‑140 (MQ=255)      gTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACCTCTGCATTAACACCATGCTCGCCATCGGCCCACGCca                                                                                                                        >  1:399393/1‑140 (MQ=255)      gTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCca                                                                                                                        <  1:1007779/140‑1 (MQ=255)      gTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCca                                                                                                                        >  2:815720/1‑140 (MQ=255)      gTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCca                                                                                                                        >  2:615442/1‑140 (MQ=255)      gTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCca                                                                                                                        <  1:728037/140‑1 (MQ=255)                tGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGATCGGCAACGGCCCCCGCCACACCATCGCa                                                                                                              >  1:318142/1‑140 (MQ=255)                    ttGCGCCAGCGTCAATTCAATGTCACCGGTGCCTGCCGGCATATCAAGTCCCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTgt                                                                                                          <  2:259000/140‑1 (MQ=255)                        gcCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTgtcgtc                                                                                                      <  2:769223/140‑1 (MQ=255)                         ccAGCGTCAATTGAATGTCACCCGTGCCGTGCGGCATCTCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCa                                                                                                     <  1:339662/140‑1 (MQ=255)                            gCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTg                                                                                                  >  1:573475/1‑140 (MQ=255)                                    tGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATa                                                                                          <  1:915842/140‑1 (MQ=255)                                    tGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATa                                                                                          <  2:104698/140‑1 (MQ=255)                                    tGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATa                                                                                          <  1:712145/140‑1 (MQ=255)                                       aTGTCACCGGTGCCTGGCGGCATATCGAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAAcc                                                                                       <  1:826536/140‑1 (MQ=255)                                       aTGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCATGCCACACGATCGCACTGTCGTTAGTGACCAGATAAcc                                                                                       >  1:282496/1‑140 (MQ=255)                                                      ggcggcATATCAAGCACCAGATAGTCGAGATCCGGCTACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGc                                                                        <  1:543672/140‑1 (MQ=255)                                                      ggcggcATATCAAGCACAAGATCGTCGAGATGCGGTCACTAGGTTTCCCGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTgt                                                                        <  2:282496/140‑2 (MQ=255)                                                           cATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGc                                                                   >  2:49055/1‑140 (MQ=255)                                                           cATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGc                                                                   >  2:982319/1‑140 (MQ=255)                                                              atCAAGCACCAGATAGTTGAGATCCGGCCACTAGGTTTCCTGCAACATCTGCATTAACGCCTTGTTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCAt                                                                <  2:392332/140‑1 (MQ=255)                                                              atCAAGCACCAGATAGTTGAGACCCGGCCACCAGGCTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCAt                                                                <  2:284236/140‑1 (MQ=255)                                                              atCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCAt                                                                <  1:982319/140‑1 (MQ=255)                                                                       cAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATaa                                                       >  2:853967/1‑140 (MQ=255)                                                                            aGTCGAGATCCGGCCACCAGGTCTCCTGCAACATCTGCACTAACGCCTGGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGt                                                  <  2:347018/140‑1 (MQ=255)                                                                              tCGAGATCCGGCCACGAGGTTTCCTACAAAATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGc                                                <  1:325321/140‑1 (MQ=255)                                                                              tCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGc                                                <  1:815720/140‑1 (MQ=255)                                                                              tCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGc                                                <  1:760384/140‑1 (MQ=255)                                                                              tCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGc                                                <  1:956589/140‑1 (MQ=255)                                                                                   aTCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCatat                                           >  2:638781/1‑140 (MQ=255)                                                                                        gCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTa                                      >  1:949255/1‑140 (MQ=255)                                                                                              aGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTcc                                <  1:638781/140‑1 (MQ=255)                                                                                                gTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCgg                              <  2:573475/140‑1 (MQ=255)                                                                                                                 cATTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATTGGTACCGTCCGGCAAAGTTGGACGCTGAt             >  1:379789/1‑140 (MQ=255)                                                                                                                  aTTAACGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGAtt            >  1:855842/1‑140 (MQ=255)                                                                                                                    tAACACATTGCTCGCCATCGGCACACGCCTCCCCATCGCATTGTCGTCCGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACACAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGAtttt          <  2:318142/140‑1 (MQ=255)                                                                                                                      aCGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCt        <  1:49055/140‑1 (MQ=255)                                                                                                                      aCGCCTTGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCt        >  1:938105/1‑140 (MQ=255)                                                                                                                           ttGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGcc   <  1:732646/140‑1 (MQ=255)                                                                                                                            tGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGccc  <  1:979405/140‑1 (MQ=255)                                                                                                                            tGCTCGCCATCGGCCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGGACCGTCCCGCGTAGTTGGACGGTGATTTTCTGCGccc  >  1:493174/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                               CACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGGCCACAAGGTTTCCTGCAACATCTGCATTAACGCCTTGCTCGCCATCGGTCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCC  >  NC_000913/2193442‑2193704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑