Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,202,630 A→G G784G (GGA→GGG)  yehI → DUF4132 domain‑containing protein YehI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,202,6300AG97.3% 124.2 / NA 37G784G (GGA→GGG) yehIDUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (17/19);  minor base C (1/0);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCA‑GGGGG‑GACGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGACAACTGGGCATTTCTGGCGTTAA  >  NC_000913/2202503‑2202766
                                                                                                                                 |                                                                                                                                        
ttCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAg                                                                                                                               <  1:97409/140‑1 (MQ=255)
ttCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAg                                                                                                                               >  2:554878/1‑140 (MQ=255)
ttCCAAAAGCCCCTGATTTCCGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAg                                                                                                                               <  1:86594/140‑1 (MQ=255)
   cAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCgg                                                                                                                            <  1:229713/140‑1 (MQ=255)
         ccccTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGAGAAATGCTGCGCATTACCCA‑GGGGGCGGCGGTTTTATAGCGGGCTGGa                                                                                                                      >  2:453025/1‑140 (MQ=255)
          cccTGATTTCTGGCATCCGCAAATGCGGTACAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGaa                                                                                                                     <  1:453025/140‑1 (MQ=255)
            cTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGaaca                                                                                                                   <  1:126828/140‑1 (MQ=255)
                           cGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTg                                                                                                    <  1:792901/140‑1 (MQ=255)
                                                   ttATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCttt                                                                            <  1:781538/140‑1 (MQ=255)
                                                        aCTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCt                                                                       <  1:527459/140‑1 (MQ=255)
                                                            ataatCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGa                                                                   >  2:164484/1‑140 (MQ=255)
                                                             taatCAAACTGTTACGAATGACGCTCTGGGAATTATCCGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGAt                                                                  <  1:395190/140‑1 (MQ=255)
                                                                    aCTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCa                                                           >  1:61873/1‑140 (MQ=255)
                                                                    aCTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGACAGCTTTTGCCTGGGATCTCTTCa                                                           >  2:348423/1‑140 (MQ=255)
                                                                     cTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAATGTTCTGCCAGCCACAAACGCTGGCAGCTTTAGCCTGGGATCTCTTCAc                                                          >  2:468344/1‑140 (MQ=255)
                                                                        ttACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCACCTTTTGCCTGGGATCTCTTCACTGc                                                       >  1:366629/1‑140 (MQ=255)
                                                                        ttACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCAACACACGTTGGCAGCTATTGCCTGGGATCTCTTCACTGc                                                       >  2:348110/1‑140 (MQ=255)
                                                                         tACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCg                                                      >  1:733552/1‑140 (MQ=255)
                                                                          aCCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGt                                                     >  1:3583/1‑140 (MQ=255)
                                                                             aaTGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGc                                                  <  1:828035/140‑1 (MQ=255)
                                                                              aTGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGc                                                       >  2:552794/1‑134 (MQ=255)
                                                                              aTGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGc                                                       <  1:552794/134‑1 (MQ=255)
                                                                                gACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGTTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGcaac                                               <  1:554878/140‑1 (MQ=255)
                                                                                gACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGt                                                     >  1:609898/1‑134 (MQ=255)
                                                                                gACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGt                                                     <  2:609898/134‑1 (MQ=255)
                                                                                  cGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGc                                                  <  2:6962/135‑1 (MQ=255)
                                                                                  cGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGc                                                  >  1:6962/1‑135 (MQ=255)
                                                                                       tGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GCCGTTTTTATAGCGGCCTGGAACAACTGAAAACGTCCTGCCAGCCACAAACCCTGGCAGCTTTCGGCTTGGATCTCTTCACACCGTGGCAACAAGCTgg                                        >  2:470477/1‑140 (MQ=255)
                                                                                            aTTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGccc                                   <  2:846640/140‑1 (MQ=255)
                                                                                               aTCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCg                                <  1:83149/140‑1 (MQ=255)
                                                                                                   gCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATGTCTTCACTGCGCGGGAACAAGATGGTGCCCCCGCaaa                            >  1:434838/1‑140 (MQ=255)
                                                                                                   gCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCaaa                            >  1:720244/1‑140 (MQ=255)
                                                                                                       aaTGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGAc                        <  2:720244/140‑1 (MQ=255)
                                                                                                              cgcTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACCAGCCGGGGCCCCCGCAAAAGACAACTggg                 >  1:361571/1‑140 (MQ=255)
                                                                                                                     cccagggggg‑gGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGACAACTGGGCATTTCt          <  2:3583/135‑1 (MQ=255)
                                                                                                                     cccagggggg‑gGCGTCTTTATAGCGAGCTAGAACAACCGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGACAACTGGGCATTTCt          <  1:407742/135‑1 (MQ=255)
                                                                                                                        agggggg‑gGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGACAACTGGGCATTTCTGGc       >  1:974278/3‑140 (MQ=255)
                                                                                                                             gg‑ggCGTTTTTATAGCGTGCTGGAACAACTGAAAATGTTTTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGACAACTGGGCATTTCTGGCGTTaa  <  2:466023/140‑1 (MQ=255)
                                                                                                                             gg‑ggCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGTAACAAGCTGGTGCCCCAGCAAAAGATAACTAGGCATTTCTGGCGTTaa  >  2:420025/1‑140 (MQ=255)
                                                                                                                             gg‑ggCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGACAACTGGGCATTTCTGGCGTTaa  >  2:626763/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                        
TTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCA‑GGGGG‑GACGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGACAACTGGGCATTTCTGGCGTTAA  >  NC_000913/2202503‑2202766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑