Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,286	T→C	G149G (GGT→GGC)	nfo →	endonuclease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,286	0	T	C	100.0%	130.7 / NA	38	G149G (GGT→GGC)	nfo	endonuclease IV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

TCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGTCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCC  >  NC_000913/2251149‑2251421                                                                                                                                          |                                                                                                                                        tctttGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCa                                                                                                                                       <  2:51310/140‑1 (MQ=255)  ctttGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTAGATAAAACGCAATGTGGGACAGCGGGGATAGAAAACACCGCCGGCCAg                                                                                                                                      >  2:288965/1‑140 (MQ=255)      gCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCa                                                                                                                                  <  2:786159/140‑1 (MQ=255)      gCTCAACTTCCACCCCGGCAGCCATCTGACGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCa                                                                                                                                  <  1:259873/140‑1 (MQ=255)       cTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAg                                                                                                                                 <  2:566546/140‑1 (MQ=255)       cTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAg                                                                                                                                 >  2:298497/1‑140 (MQ=255)              tCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTa                                                                                                                          <  2:100608/140‑1 (MQ=255)              tCCACCCTGGCAGCCATCTGATCCAGATTTCGGAATAGGTTTGCCTTGTGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTa                                                                                                                          <  2:343878/140‑1 (MQ=255)                                      aGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTcgcg                                                                                                  <  2:710161/140‑1 (MQ=255)                                       gATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCgcgg                                                                                                 <  2:889489/140‑1 (MQ=255)                                       gATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCgcgg                                                                                                 <  2:834336/140‑1 (MQ=255)                                          ttCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGa                                                                                              >  2:831870/1‑140 (MQ=255)                                                    gATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACgg                                                                                    >  1:1142/1‑140 (MQ=255)                                                             gcgcGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGa                                                                           <  2:875418/140‑1 (MQ=255)                                                                         gAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGt                                                               <  1:402862/140‑1 (MQ=255)                                                                               aTCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGTTTATCGAAGGCATGGAAGATAGATCCCGCGTCGGCGt                                                         >  1:444025/1‑140 (MQ=255)                                                                               aTCAACATTGCGCTGGAGAAAACTCAAGGTGTGACGGCGGTGATAGAAGACGCCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGt                                                         <  2:444025/140‑1 (MQ=255)                                                                                tCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTc                                                        <  1:831870/140‑1 (MQ=255)                                                                                 cAACATTGCGCTGGATAAAACTTAACGTGCGACAGCGATGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCt                                                       <  1:298497/140‑1 (MQ=255)                                                                                       tGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTg                                                 >  1:518483/1‑140 (MQ=255)                                                                                        gcgcTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGa                                                >  1:611390/1‑140 (MQ=255)                                                                                              gATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGCGGAAGATCAATCCCGCCTCGGAGTCTGCATTGATACCTg                                          >  2:389726/1‑140 (MQ=255)                                                                                                   aaCTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATg                                     >  2:130433/1‑140 (MQ=255)                                                                                                    aCTCAAGGTGTGACAGGTGTGATAGAAAACACCGCTGGCCAGGGCAGTCACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGc                                    <  2:276839/140‑1 (MQ=255)                                                                                                    aCTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGc                                    <  2:820731/140‑1 (MQ=255)                                                                                                        aaGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTc                                >  2:736606/1‑140 (MQ=255)                                                                                                        aaGGTGTGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCAGGGTCGGCGTCTGCATTGATACCTGCAATGCTTTc                                >  2:456931/1‑140 (MQ=255)                                                                                                           gtgtGACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCt                             <  2:772397/140‑1 (MQ=255)                                                                                                             gtgACAGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGc                           <  2:775036/140‑1 (MQ=255)                                                                                                                  aGCGGTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGt                      >  1:551168/1‑140 (MQ=255)                                                                                                                      gTGATAGAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGa                  >  1:614352/1‑140 (MQ=255)                                                                                                                            gAAAACACCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCg            <  2:518483/140‑1 (MQ=255)                                                                                                                                acacCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACt        >  1:854306/1‑140 (MQ=255)                                                                                                                                 cacCGCCGGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTc       >  2:891599/1‑140 (MQ=255)                                                                                                                                    cgccgGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGACATGCTTACGCAGCTGGGTATCATTTGCGTACTCCAg    >  1:475136/1‑140 (MQ=255)                                                                                                                                     gccgGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCGATGCTTTGGCTTCCCGGTATGGTTTGCGTACTCCAGc   >  1:402163/1‑140 (MQ=255)                                                                                                                                     gccgGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGc   >  2:105618/1‑140 (MQ=255)                                                                                                                                      ccgGCCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGcc  >  2:751341/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAGCGGTGATAGAAAACACCGCCGGTCAGGGCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCC  >  NC_000913/2251149‑2251421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑