BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,447	G→A	intergenic (‑151/+272)	psuK ← / ← fruA	putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,447	0	G	A	100.0%	433.5 / NA	125	intergenic (‑151/+272)	psuK/fruA	putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (68/57); total (68/57)

Only 100 of 125 total aligned reads displayed.

AAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAG  >  NC_000913/2259312‑2259579

aaaaaaTTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAg                                                                                                                                  >  2:877999/1‑140 (MQ=255)

        aCCAACCCTGTCAATCCACAAGAATATAGATACTGCAGAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGc                                                                                                                          >  2:45764/1‑140 (MQ=255)

        aCCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTCTACGTAAATTAAGTTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGc                                                                                                                          >  2:341492/1‑140 (MQ=255)

           aaCCCTGTCAATCCATAAGAATTTATATACTGCAAAGCCGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAgg                                                                                                                       <  1:484035/140‑1 (MQ=255)

                 gTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTcaca                                                                                                                 >  2:196057/1‑140 (MQ=255)

                       ccACAAGAATATAGATACTGCAAAGTCGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGc                                                                                                           <  1:341492/140‑1 (MQ=255)

                         acaAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGa                                                                                                         <  2:1014960/140‑1 (MQ=255)

                            aGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGGCAGAGAGGGAAACTGACCAGATTTGGGCAGCCTCACAAAATGCGATTa                                                                                                      >  2:303852/1‑140 (MQ=255)

                                     aTACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGa                                                                                             >  1:68283/1‑140 (MQ=255)

                                     aTACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGa                                                                                             >  1:883856/1‑140 (MQ=255)

                                       aCTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAAt                                                                                           >  1:170480/1‑140 (MQ=255)

                                       aCTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAAt                                                                                           >  1:713446/1‑140 (MQ=255)

                                       aCTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAAATTTGAGCAGGCTCACTAAATTCGATTAAATGCCTGAAt                                                                                           >  2:357641/1‑140 (MQ=255)

                                        cTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAAtt                                                                                          >  2:97114/1‑140 (MQ=255)

                                        cTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAAtt                                                                                          >  1:733361/1‑140 (MQ=255)

                                        cTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAAtt                                                                                          >  1:780663/1‑140 (MQ=255)

                                        cTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATGTGAGCAGACTCCCACAATGCGATTAAATGCCTGAAtt                                                                                          >  1:322108/1‑140 (MQ=255)

                                        cTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGGTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAAtt                                                                                          >  2:831559/1‑140 (MQ=255)

                                         tGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTa                                                                                         >  2:171138/1‑140 (MQ=255)

                                         tGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTa                                                                                         >  1:536837/1‑140 (MQ=255)

                                         tGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTa                                                                                         >  1:122359/1‑140 (MQ=255)

                                           cAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACa                                                                                       >  2:964163/1‑140 (MQ=255)

                                           cAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACa                                                                                       >  1:675407/1‑140 (MQ=255)

                                                  ggTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGa                                                                                <  2:873831/140‑1 (MQ=255)

                                                   gTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAg                                                                               >  1:165740/1‑140 (MQ=255)

                                                            tgtgTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTtgtg                                                                      <  2:170480/140‑1 (MQ=255)

                                                              tgtTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGTTTAAATGACTGGATGACAAAAGCGAGGAATTTGTGAc                                                                    >  1:269775/1‑140 (MQ=255)

                                                              tgtTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGAc                                                                    >  1:939199/1‑140 (MQ=255)

                                                               gtTCATACTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACg                                                                   <  1:171138/140‑1 (MQ=255)

                                                                ttCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGGTTAGATGACTGAATTACAAAAGCGAGGAATTTGTGACgg                                                                  >  2:341645/1‑140 (MQ=255)

                                                                 tCATATTTAAACATGTGGTCATAGTATGCATCTTTCACGCAAAGTAAACTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACggg                                                                 <  2:269775/140‑1 (MQ=255)

                                                                 tCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACggg                                                                 <  1:45764/140‑1 (MQ=255)

                                                                 tCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACggg                                                                 <  2:675407/140‑1 (MQ=255)

                                                                 tCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACggg                                                                 <  2:607322/140‑1 (MQ=255)

                                                                 tCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACggg                                                                 <  1:818125/140‑1 (MQ=255)

                                                                 tCATATTTAAACATGGGCTCATTGTGTGCTTGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACggg                                                                 <  1:303852/140‑1 (MQ=255)

                                                                     atTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACggg                                                                 <  1:808726/136‑1 (MQ=255)

                                                                      tttAAACATGTGCTCATAGTATTCATGTTTTCCGTAAAGCAAATTACCCCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGca                                                            <  2:322108/140‑1 (MQ=255)

                                                                      tttAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGca                                                            <  2:68283/140‑1 (MQ=255)

                                                                      tttAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGca                                                            <  2:246293/140‑1 (MQ=255)

                                                                      tttAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGca                                                            <  1:980728/140‑1 (MQ=255)

                                                                      tttAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGca                                                            <  2:122359/140‑1 (MQ=255)

                                                                      tttAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGca                                                            <  2:812833/140‑1 (MQ=255)

                                                                      tttAAACATGTGCTCATAGTATCCACGTTTTACGTAATGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGca                                                            <  1:497846/140‑1 (MQ=255)

                                                                          aaCATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGTATTACAATAGCGAGGAATTTGTGACGGGATGCacaga                                                        >  1:373313/1‑140 (MQ=255)

                                                                          aaCATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCacaga                                                        >  2:724062/1‑140 (MQ=255)

                                                                              tgtgCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCacagaacag                                                    <  2:883856/140‑1 (MQ=255)

                                                                               gtgCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACggg                                                                 >  2:20018/1‑126 (MQ=255)

                                                                               gtgCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACggg                                                                 <  1:20018/126‑1 (MQ=255)

                                                                               gtgCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGAAGGGATGCacagaacaga                                                   >  2:441508/1‑140 (MQ=255)

                                                                               gtgCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAAAGCCTGAATAACAGAAGGAAGGAATTTGTGAGGGGATGCagagaacaga                                                   >  2:497846/1‑140 (MQ=255)

                                                                                tgCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCAcagaacagaa                                                  >  2:165864/1‑140 (MQ=255)

                                                                                tgCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCAcagaacagaa                                                  >  1:759665/1‑140 (MQ=255)

                                                                                tgCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCAcagaacagaa                                                  >  2:81923/1‑140 (MQ=255)

                                                                                tgCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCAcagaacagaa                                                  <  1:709665/140‑1 (MQ=255)

                                                                                tgCTCATAGTATGCATGTTTTAAGTAAAGTACATTACCGACAGAGAGGAAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCAcagaacagaa                                                  <  2:307275/140‑1 (MQ=255)

                                                                                 gCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACagaacagaac                                                 >  1:65499/1‑140 (MQ=255)

                                                                                  cTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAgaacagaaca                                                >  2:762892/1‑140 (MQ=255)

                                                                                     aTAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGca                                                            >  1:542670/1‑125 (MQ=255)

                                                                                     aTAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGca                                                            <  2:542670/125‑1 (MQ=255)

                                                                                     aTAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCacag                                                         >  2:716685/1‑128 (MQ=255)

                                                                                       aGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATaaa                                           >  1:654487/1‑140 (MQ=255)

                                                                                         tATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATaaaaa                                         >  2:924726/1‑140 (MQ=255)

                                                                                          aTGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATaaaaaa                                        >  1:123775/1‑140 (MQ=255)

                                                                                          aTGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATaaaaaa                                        >  2:959905/1‑140 (MQ=255)

                                                                                          aTGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATaaaaaa                                        >  1:805236/1‑140 (MQ=255)

                                                                                           tGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGATTTTGTGATGGGGTGGACAGAAGAGAACAATAAAAAAg                                       >  1:286565/1‑140 (MQ=255)

                                                                                           tGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAg                                       >  2:73730/1‑140 (MQ=255)

                                                                                           tGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAg                                       >  2:828928/1‑140 (MQ=255)

                                                                                             cATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCacagaacag                                                    >  2:674932/1‑125 (MQ=255)

                                                                                             cATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAggg                                     <  1:112690/140‑1 (MQ=255)

                                                                                             cATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAggg                                     <  1:924726/140‑1 (MQ=255)

                                                                                              aTGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGc                                    <  1:762892/140‑1 (MQ=255)

                                                                                              aTGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGc                                    <  2:801653/140‑1 (MQ=255)

                                                                                                  tttACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGaa                                <  2:123775/140‑1 (MQ=255)

                                                                                                   ttACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGaaa                               >  1:843186/1‑140 (MQ=255)

                                                                                                    tACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACAGGATGCAGAGAACAAAACAATGAAAAAGGGCAGaaaa                              >  1:362057/1‑140 (MQ=255)

                                                                                                         aaagtaaaTTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATc                         <  1:664661/140‑1 (MQ=255)

                                                                                                           agtaaaTTACCGCCAGAGAGGCTAAATGAGCCGATTTGATCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTg                       <  2:270313/140‑1 (MQ=255)

                                                                                                           agtaaaTTACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTg                       >  2:68515/1‑140 (MQ=255)

                                                                                                                 ttACCGCCAGAGAGGGAAAATGAGCTGATTTGAGCAGCCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                 <  1:439000/140‑1 (MQ=255)

                                                                                                                 ttACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                 <  1:165864/140‑1 (MQ=255)

                                                                                                                 ttACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                 <  1:97114/140‑1 (MQ=255)

                                                                                                                 ttACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                 <  2:780663/140‑1 (MQ=255)

                                                                                                                 ttACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                 <  1:195823/140‑1 (MQ=255)

                                                                                                                 ttACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                 <  2:65499/140‑1 (MQ=255)

                                                                                                                 ttACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                 <  2:654487/140‑1 (MQ=255)

                                                                                                                 ttACCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCCCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                 <  2:469919/140‑1 (MQ=255)

                                                                                                                   aCCGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAg               <  1:196057/140‑1 (MQ=255)

                                                                                                                     cGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGa             >  2:762243/1‑140 (MQ=255)

                                                                                                                     cGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGa             >  2:235058/1‑140 (MQ=255)

                                                                                                                     cGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGa             <  1:947887/140‑1 (MQ=255)

                                                                                                                     cGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGa             >  2:947887/1‑140 (MQ=255)

                                                                                                                     cGCCAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGa             <  1:81923/140‑1 (MQ=255)

                                                                                                                       ccAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                 >  2:171757/1‑134 (MQ=255)

                                                                                                                       ccAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGaaa           >  1:623904/1‑140 (MQ=255)

                                                                                                                       ccAGAGAGGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGaaa           >  1:906361/1‑140 (MQ=255)

                                                                                                                           agagGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAAt          >  2:142421/1‑137 (MQ=255)

                                                                                                                           agagGCAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAAt          <  1:142421/137‑1 (MQ=255)

                                                                                                                                cAAAATGAGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAg  <  2:713446/140‑1 (MQ=255)

AAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAG  >  NC_000913/2259312‑2259579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`