Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,295,780	T→C	N181D (AAC→GAC)	ccmC ←	heme trafficking system membrane protein CcmC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,295,780	0	T	C	100.0%	112.7 / NA	33	N181D (AAC→GAC)	ccmC	heme trafficking system membrane protein CcmC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/19);  total (14/19)

AAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAA  >  NC_000913/2295645‑2295911                                                                                                                                        |                                                                                                                                    aaaTCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCccac                                                                                                                                 <  1:953242/140‑1 (MQ=255)  aaTCAAATGACGCATCGCCATCGGCGTCAGATTTGCAGACAGGAGCAGAAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCccacc                                                                                                                                <  2:500215/140‑1 (MQ=255)   aTCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCccacca                                                                                                                               <  2:847291/140‑1 (MQ=255)   aTCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCccacca                                                                                                                               >  2:779411/1‑140 (MQ=255)        aTTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCa                                                                                                                          <  2:985139/140‑1 (MQ=255)        aTTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCa                                                                                                                          <  1:891950/140‑1 (MQ=255)            cgcatccgcatcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGa                                                                                                                      <  1:995812/140‑1 (MQ=255)                         gcgtcagcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACGCCACGGAGTAATGAATAATc                                                                                                         <  2:210628/140‑1 (MQ=255)                          cgtcagcgcGGCAGACCAGAGCAGGAAGCCAAATACCGACCAGCGCAGGGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCg                                                                                                        <  2:463824/140‑1 (MQ=255)                           gtcagcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCgg                                                                                                       >  1:164751/1‑140 (MQ=255)                            tcagcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGc                                                                                                      >  2:926803/1‑140 (MQ=255)                              agcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAg                                                                                                    >  2:911918/1‑140 (MQ=255)                               gcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCGTGATGCAGAGTGGCCCTCCACTCCAGGGAGGAATGAATAATCGGCAGa                                                                                                   >  2:286798/1‑140 (MQ=255)                               gcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGa                                                                                                   >  1:572732/1‑140 (MQ=255)                                   ggCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTca                                                                                               <  1:336/140‑1 (MQ=255)                                   ggCAGACAGGAGCAGCAAGCCACAACTCGCCCAGCGCCGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTca                                                                                               <  1:262253/140‑1 (MQ=255)                                    gCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCAACCACTCGACGGAGCAATGAAGAATCGGCAGATTcac                                                                                              >  1:497421/1‑140 (MQ=255)                                            gAGCAGGAAGCCAAACATCGACTAGCGCAACGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCaa                                                                                      <  1:286798/140‑1 (MQ=255)                                                 ggAAGCCAAAAATCGCCCAGCGTAGAGGCTAACGCATAGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGc                                                                                 <  1:297634/140‑1 (MQ=255)                                                    aGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCAcc                                                                              <  1:245954/140‑1 (MQ=255)                                                                               aaCGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGa                                                   >  1:985713/1‑140 (MQ=255)                                                                                  gCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGAcgg                                                <  2:572732/140‑1 (MQ=255)                                                                                  gCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGAcgg                                                <  1:894546/140‑1 (MQ=255)                                                                                    aTCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGTATACCTGCCGCACGGCCCGCCAGAcggcg                                              <  2:164751/140‑1 (MQ=255)                                                                                     tCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGAcggcgg                                             <  2:175438/140‑1 (MQ=255)                                                                                                aTACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGc                                  >  2:829894/1‑140 (MQ=255)                                                                                                  aCTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCGGCCGCACTGCCAGCTAGACGGCGGTCGTCGAAGGCGt                                >  1:482461/1‑140 (MQ=255)                                                                                                     ttGCTGCATCCGCGTTGGTCCCCGATGCAGGTTGTCCCACCACTCCACTGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGcc                             <  2:354327/140‑1 (MQ=255)                                                                                                      tgctgcATCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACTGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCca                            >  1:251406/1‑140 (MQ=255)                                                                                                             tCCGCGTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCa                     >  2:131186/1‑140 (MQ=255)                                                                                                                 cgTTGATCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATca                 <  1:779411/140‑1 (MQ=255)                                                                                                                      aTCCCTGATGCAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACAccc            >  1:732399/1‑140 (MQ=255)                                                                                                                                cAGGGTGTCCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACaaaa  >  2:146094/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    AAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAA  >  NC_000913/2295645‑2295911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑