Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,312,660	G→A	N30N (AAC→AAT)	ompC ←	outer membrane porin C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,312,660	0	G	A	98.8%	281.3 / NA	83	N30N (AAC→AAT)	ompC	outer membrane porin C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (41/41);  minor base C (0/1);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCAC  >  NC_000913/2312523‑2312795                                                                                                                                          |                                                                                                                                        tGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTGCTGTACCGTGCAGATCTAATttatt                                                                                                                                       >  1:472478/1‑140 (MQ=255) tGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttatt                                                                                                                                       >  2:69477/1‑140 (MQ=255)  ggCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCATTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATttattg                                                                                                                                      >  2:612476/1‑140 (MQ=255)  ggCCGT‑ACCTGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGGAGGCCGTGTACGTTACCGTACTGATCTAATTTATTGc                                                                                                                                     >  1:424376/1‑140 (MQ=255)        taCCTGTCAGCTGGTCACTAACCTGAGTTTCCCCTGTGATGCCAAGACCCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCt                                                                                                                                <  2:424376/139‑1 (MQ=255)             gTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTttgtt                                                                                                                           >  1:560610/1‑140 (MQ=255)             gTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTttgtt                                                                                                                           >  1:213433/1‑140 (MQ=255)             gTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTATACGTTACCGTACAGATCTAAGTTATTGCCGTCTttgtt                                                                                                                           >  2:309164/1‑140 (MQ=255)                  cTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTaaa                                                                                                                      >  1:58670/1‑140 (MQ=255)                        aGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTcag                                                                                                                <  1:34115/140‑1 (MQ=255)                                      cACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcg                                                                                                  <  1:502539/140‑1 (MQ=255)                                           ttGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGc                                                                                             >  1:60312/1‑140 (MQ=255)                                            tGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGATATAGTGCAGGCCGTCTACTTTACCGTACAGCTCTAATTTATTGCCGTGTTTGTTGGAAAGTTGAGCAGCTTTTGCTGCGCCTGCt                                                                                            >  1:273404/1‑140 (MQ=255)                                                  cAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagc                                                                                      <  2:765098/140‑1 (MQ=255)                                                   aaGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagca                                                                                     <  1:537382/140‑1 (MQ=255)                                                   aaGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagca                                                                                     >  2:542684/1‑140 (MQ=255)                                                   aaGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCAGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagca                                                                                     >  2:721724/1‑140 (MQ=255)                                                           tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTTAGCAGCGCTTGGTGGGCCTGCTACCAGCAGAGCTggg                                                                             >  1:349505/1‑140 (MQ=39)                                                           tGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTAAGCAGCGTTTGCTGCGCCTGCCACCAGCAGAGCTggg                                                                             >  1:440526/1‑140 (MQ=255)                                                                    ggTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg                                                                              >  2:740760/1‑130 (MQ=255)                                                                    ggTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg                                                                              <  1:740760/130‑1 (MQ=255)                                                                    ggTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg                                                                    <  1:147212/140‑1 (MQ=255)                                                                    ggTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTGTGCTGCGGCTGGTAACAGCAGGGCTGGGACCaggagg                                                                    >  1:288722/1‑140 (MQ=39)                                                                     gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagca                                                                                     <  1:858117/122‑1 (MQ=255)                                                                     gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagca                                                                                     >  2:858117/1‑122 (MQ=255)                                                                     gTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAggg                                                                   >  2:867935/1‑140 (MQ=255)                                                                      tCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCAGCTTCCTGCAGAGCTGGGACCAGGAGGGa                                                                  >  1:258834/1‑140 (MQ=255)                                                                       cGCCATCTACATCTTTGTTGTCCGATAAACAGTGCAGTCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGAc                                                                 <  1:446503/140‑1 (MQ=255)                                                                       cGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGAc                                                                 <  2:560610/140‑1 (MQ=255)                                                                        gCCATCTACATCTTTCTTGTCATAGCAATAGTGCAGGTCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACa                                                                <  1:309164/140‑1 (MQ=38)                                                                              tACATCTTAGTTGTCAGTGAAAAAGTGCAGGTCGTATACATTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt                                                          <  2:472478/140‑1 (MQ=255)                                                                               aCATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt                                                         <  1:673473/140‑1 (MQ=255)                                                                                      tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGTGCCGGGACCAGGAGGGCCAGTACTTTAACTTTc                                                  >  2:479292/1‑140 (MQ=255)                                                                                      tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTc                                                  >  2:143791/1‑140 (MQ=255)                                                                                      tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTc                                                  >  1:664431/1‑140 (MQ=255)                                                                                      tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTc                                                  >  1:403832/1‑140 (MQ=255)                                                                                       gttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGTGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCa                                                 <  1:69477/140‑1 (MQ=255)                                                                                        ttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCAt                                                <  1:143791/140‑1 (MQ=255)                                                                                        ttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCAt                                                >  2:57391/1‑140 (MQ=255)                                                                                        ttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCAt                                                <  2:58670/140‑1 (MQ=255)                                                                                          gtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt                                              <  1:598296/140‑1 (MQ=255)                                                                                          gtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt                                              <  1:578044/140‑1 (MQ=255)                                                                                          gtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCAGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGt                                              <  1:721724/140‑1 (MQ=255)                                                                                              gagaAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACt                                                     >  2:520042/1‑129 (MQ=255)                                                                                              gagaAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACt                                                     <  1:520042/129‑1 (MQ=255)                                                                                              gagaAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttatt                                          >  2:613596/1‑140 (MQ=255)                                                                                                 aaaTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAc                                       <  2:213433/140‑1 (MQ=255)                                                                                                  aaTAGTGCAGGCCGTCTCCTTTTCCGTACATCTGTAATTTATTGCCGTTTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAcc                                      <  2:475657/140‑1 (MQ=255)                                                                                                  aaTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAcc                                      <  1:86250/140‑1 (MQ=255)                                                                                                  aaTAGTGCAGGCCGTATACTTTGCCGTACAGATCTAAATTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAcc                                      <  1:479292/140‑1 (MQ=255)                                                                                                   aTAGTGCAGGCCGTCTACTTTATCGTACAGATCTAATTTCTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAccc                                     <  2:285958/140‑1 (MQ=255)                                                                                                   aTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAccc                                     <  1:542684/140‑1 (MQ=255)                                                                                                    tAGTGCAGGCCGTCTACTTTACCGTACAGATTTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCct                                    <  2:403832/140‑1 (MQ=255)                                                                                                    tAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCct                                    <  2:664431/140‑1 (MQ=255)                                                                                                     aGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCctc                                   >  2:935170/1‑140 (MQ=255)                                                                                                     aGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCctc                                   <  1:613596/140‑1 (MQ=255)                                                                                                     aGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCctc                                   <  2:60312/140‑1 (MQ=255)                                                                                                     aGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCctc                                   >  2:849206/1‑140 (MQ=255)                                                                                                     aGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCACGAGGGACAGTAGTTCAACTTTGATGTTATTAACCctc                                   >  1:475657/1‑140 (MQ=38)                                                                                                       tGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCctc                                   <  1:110234/138‑1 (MQ=255)                                                                                                       tGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCctc                                   >  2:110234/1‑138 (MQ=255)                                                                                                       tGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTg                                 >  2:85035/1‑140 (MQ=255)                                                                                                       tGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTg                                 >  2:148643/1‑140 (MQ=255)                                                                                                       tGCAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTg                                 >  1:653366/1‑140 (MQ=255)                                                                                                         cAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACTGTACTTTAACTTTCATGTTATTAACCCTCTGtt                               >  2:794762/1‑140 (MQ=255)                                                                                                         cAGGCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGtt                               >  2:791640/1‑140 (MQ=255)                                                                                                           ggCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTtat                             >  1:44656/1‑140 (MQ=255)                                                                                                            gCCGTCTACTTTACCGTCCAGATCTAATTTAATGCCGTCGTTGTTGTACACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTtata                            <  2:349505/140‑1 (MQ=255)                                                                                                            gCCGTCTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGAAAGTACTTGAACGTTGATGTTACTAACCCTCTGTtata                            >  1:481211/1‑140 (MQ=255)                                                                                                                 cTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGTAGGGACAGCACTATAACTTTCATGTGATTATCCCTCTGTTATATGCCt                       >  2:291400/1‑140 (MQ=38)                                                                                                                 cTACTTTACCGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCt                       >  1:886174/1‑140 (MQ=255)                                                                                                                          cGTACAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCt              >  2:773664/1‑140 (MQ=255)                                                                                                                          cGTACAGATCTAATTTATTGCCGTCTCTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCt              >  1:842737/1‑140 (MQ=255)                                                                                                                              cAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCttttt          <  1:612476/140‑1 (MQ=255)                                                                                                                              cAGATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCttttt          >  2:253797/1‑140 (MQ=255)                                                                                                                                gATCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTa        <  1:867935/140‑1 (MQ=255)                                                                                                                                 aTCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTAt       >  1:900379/1‑140 (MQ=255)                                                                                                                                 aTCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTAt       >  1:231882/1‑140 (MQ=255)                                                                                                                                  tCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATg      <  2:579870/140‑1 (MQ=255)                                                                                                                                  tCTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATg      <  1:849206/140‑1 (MQ=255)                                                                                                                                  tCTAATTTATTGCCGTCTTTGTCGTAAACTTCAGCTGCGGTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATg      <  2:273404/140‑1 (MQ=255)                                                                                                                                   cTAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGc     <  1:935170/140‑1 (MQ=255)                                                                                                                                    tAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGcc    <  1:253797/140‑1 (MQ=255)                                                                                                                                    tAATTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGATACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGcc    <  1:186377/140‑1 (MQ=255)                                                                                                                                     aaTTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACTGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCa   <  1:794762/140‑1 (MQ=255)                                                                                                                                     aaTTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCa   <  2:842737/140‑1 (MQ=255)                                                                                                                                     aaTTTATTGCCGTCTTTCTTGTAAACTTCAGGAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCa   <  2:440526/140‑1 (MQ=255)                                                                                                                                      aTTTATTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAACCTCTGTAATAGGCGTTTATTAGCTTTTTTATGCCAc  >  1:491886/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTATGCCAC  >  NC_000913/2312523‑2312795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑