Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,317,538	T→C	E780G (GAG→GGG)	rcsC ←	sensory histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,317,538	0	T	C	100.0%	236.9 / NA	66	E780G (GAG→GGG)	rcsC	sensory histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/40);  total (26/40)

CATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTA  >  NC_000913/2317402‑2317671                                                                                                                                         |                                                                                                                                      catATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCGACAATGCCTGTAACTCATGCGGAGCGGCCAGACTGTGTACCCACcccc                                                                                                                                    >  2:300795/1‑140 (MQ=255)    atCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTg                                                                                                                                 <  1:61621/140‑1 (MQ=255)    atCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTg                                                                                                                                 <  2:577800/140‑1 (MQ=255)      cGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTAATGCGGAGCAGACACACTGTGCTCCCACCCCCCTGgc                                                                                                                               >  1:499654/1‑140 (MQ=255)       gTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGgcg                                                                                                                              <  1:132722/140‑1 (MQ=255)        tCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCTGCCTCACTGTGTAACCACCTCCCTGgcgc                                                                                                                             >  1:357077/1‑140 (MQ=255)         cATTATCGCTGACCGCTTTGTCTGTTGACGGCAGAACGTCAGCAGGATGGGCGCTCTCCATCTCGATCAAATAAATACGCTCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCt                                                                                                                            <  2:392760/140‑1 (MQ=255)             aTCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTtct                                                                                                                        >  2:160420/1‑140 (MQ=255)                 cTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAg                                                                                                                    <  1:84342/140‑1 (MQ=255)                 cTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAg                                                                                                                    >  1:669249/1‑140 (MQ=255)                 cTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCACTCCATCACGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAg                                                                                                                    >  2:613421/1‑140 (MQ=255)                    aCCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCt                                                                                                                            >  2:829372/1‑129 (MQ=255)                    aCCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCt                                                                                                                            <  1:829372/129‑1 (MQ=255)                    aCCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCgg                                                                                                                 <  2:116533/140‑1 (MQ=255)                    aCCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCgg                                                                                                                 <  1:667710/140‑1 (MQ=255)                    aCCGCTTTCTCCGTTGACGGCAGAGCGTTAGTACGATCGTCGCTCTCCTTCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCgg                                                                                                                 <  2:466816/140‑1 (MQ=255)                         tttGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATAc                                                                                                            <  2:953248/140‑1 (MQ=255)                             tCCGTTGATGGCAGAGACTTAGTAGGATCGTCGATTTCCTTCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTTCCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAat                                                                                                        <  2:499654/140‑1 (MQ=255)                                       gCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGcgacga                                                                                              <  1:207243/140‑1 (MQ=255)                                          gagCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAg                                                                                           <  1:579176/140‑1 (MQ=255)                                          gagCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAg                                                                                           <  1:197716/140‑1 (MQ=255)                                          gagCGTTACCAGGATCCTCGCTTTCCATCTCAATCAAATAAATACGCGGCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAg                                                                                           <  2:472829/140‑1 (MQ=255)                                           agCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGa                                                                                          >  2:64157/1‑140 (MQ=255)                                               ttAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGt                                                                                      <  1:1017434/140‑1 (MQ=255)                                                     ggATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTAc                                                                                >  2:717865/1‑140 (MQ=255)                                                       aTCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCg                                                                              >  2:755241/1‑140 (MQ=255)                                                             gCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTctgc                                                                        <  2:569230/140‑1 (MQ=255)                                                                ctcCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTctgccct                                                                     >  2:512228/1‑140 (MQ=255)                                                                ctcCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTctgccct                                                                     >  1:199297/1‑140 (MQ=255)                                                                   cATCTCGATCAAATATATACGCACCTACACTGCCGGTTGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTctgccctgcc                                                                  <  1:300795/140‑1 (MQ=255)                                                                      ctcGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAtt                                                               >  2:247149/1‑140 (MQ=255)                                                                         gATCAAATTAATACGCGCCAACTATGCCGGTAGGTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAttttt                                                            <  2:452348/140‑1 (MQ=255)                                                                         gATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAttttt                                                            <  1:717865/140‑1 (MQ=255)                                                                         gATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAttttt                                                            >  2:693787/1‑140 (MQ=255)                                                                          aTCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAtttttt                                                           <  2:199297/140‑1 (MQ=255)                                                                             aaataaatACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACt                                                        <  1:231333/140‑1 (MQ=255)                                                                             aaataaatACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACt                                                        <  1:755241/140‑1 (MQ=255)                                                                             aaataaatACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGCCACTACCGCTCTGCCCTGCCATTTTTTACt                                                        >  2:995730/1‑140 (MQ=255)                                                                              aataaataCGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTc                                                       >  1:975167/1‑140 (MQ=255)                                                                              aataaataCGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTc                                                       <  1:512228/140‑1 (MQ=255)                                                                                taaataCGCGCCAACGATGCCGGTACCTCGTGCGGAGTAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCAc                                                     <  1:334201/140‑1 (MQ=255)                                                                                taaataCGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCAc                                                     <  2:997321/140‑1 (MQ=255)                                                                                   ataCGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTAc                                                  <  1:584398/140‑1 (MQ=255)                                                                                   ataCGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTAc                                                  <  2:236037/140‑1 (MQ=255)                                                                                     aCGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCt                                                <  2:690957/140‑1 (MQ=255)                                                                                        cgcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGt                                             >  2:919592/1‑140 (MQ=255)                                                                                         gcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATGTGGCGACGACAGAAGGTCAATACGGCTATGCCCTGCCATTTATTACTCACTACCTCGTc                                            >  2:308744/1‑140 (MQ=255)                                                                                         gcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACGGAAGGTCACTACGGATATGCCCTGCAATTTATTACTCACAACCTCGTc                                            >  2:493375/1‑140 (MQ=255)                                                                                         gcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGt                                             <  1:93438/139‑1 (MQ=255)                                                                                         gcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGt                                             >  2:93438/1‑139 (MQ=255)                                                                                         gcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTc                                            >  1:215697/1‑140 (MQ=255)                                                                                         gcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCGGCGATTTTTAACTCACTACCTCGTc                                            >  1:263123/1‑140 (MQ=255)                                                                                           caacaaTGCCGGTAGCTCATGCGGAGCAGCCCCACAGTGTACCCACCTCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAg                                          <  2:357077/140‑1 (MQ=255)                                                                                              caaTGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGa                                       <  1:995730/140‑1 (MQ=255)                                                                                                   ccGGTAGCTCATGCGGAGCAGCCACACTGTGTACCTACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTg                                        <  2:550534/134‑1 (MQ=255)                                                                                                   ccGGTAGCTCATGCGGAGCAGCCACACTGTGTACCTACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTg                                        >  1:550534/1‑134 (MQ=255)                                                                                                        aGCTCATGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATc                             >  1:673689/1‑140 (MQ=255)                                                                                                             aTGCGGAGCAGCCACACTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATCTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCgg                        >  2:404675/1‑140 (MQ=255)                                                                                                                           cacTGTGTACCCCCCACCCTGGCGCGTTCGCCAGCGGTATACCAATGTGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGc          <  1:308744/140‑1 (MQ=255)                                                                                                                           cacTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGTAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGc          <  1:815985/140‑1 (MQ=255)                                                                                                                            acTGTGTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCAATACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGcc         <  2:669249/140‑1 (MQ=255)                                                                                                                              tgtgTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCt       <  1:779255/140‑1 (MQ=255)                                                                                                                              tgtgTACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCt       <  2:975167/140‑1 (MQ=255)                                                                                                                                tgtACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTc     <  2:215697/140‑1 (MQ=255)                                                                                                                                  tACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGGCGACAGAAGGCCACTACCCCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACCCATCTTCGGGAGTCGGTTCCTGCCCTTCGt   >  2:862113/1‑140 (MQ=255)                                                                                                                                  tACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGt   <  1:919592/140‑1 (MQ=255)                                                                                                                                  tACCCACCCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGAACAAAACATCTTCGGGAGACGGTTCCTGCCCTTCGt   >  2:300869/1‑140 (MQ=255)                                                                                                                                   acccacccCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTa  <  1:160420/140‑1 (MQ=255)                                                                                                                                   aCCCATCCCCCTGGCGCCTTCTTCAGCGGAATACCGATACGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTa  <  2:314935/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTA  >  NC_000913/2317402‑2317671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑