Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	242,717	A→T	I196N (ATC→AAC)	fadE ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	242,717	0	A	T	100.0%	77.6 / NA	24	I196N (ATC→AAC)	fadE	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/13);  total (11/13)

GTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGATCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGATGCTCTTTAAGGTACGCCCACAACTCC  >  NC_000913/242586‑242845                                                                                                                                    |                                                                                                                                 gTGCAAAGCAGGGGAACTCCTGCCCCCGCGCCAGACGAGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTc                                                                                                                          <  1:369423/140‑1 (MQ=255)       aGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCg                                                                                                                    >  2:766964/1‑140 (MQ=255)        gCAGGGGATCTCCTGACCACGCCCCAGACGCTGCATATACTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCgg                                                                                                                   <  2:281860/140‑1 (MQ=255)                    cTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTgca                                                                                                       <  1:102061/140‑1 (MQ=255)                    cTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTgca                                                                                                       <  2:660533/140‑1 (MQ=255)                               ccAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGAc                                                                                            <  2:163479/140‑1 (MQ=255)                               ccAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGAc                                                                                            <  2:857041/140‑1 (MQ=255)                               ccAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGAc                                                                                            <  1:915043/140‑1 (MQ=255)                                      cgGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCAt                                                                                     >  2:197601/1‑140 (MQ=255)                                                       tCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTACAGCACGCGAGACTCGGCATACGCCGAGAACTCCAGc                                                                    >  1:411249/1‑140 (MQ=255)                                                          gCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCccg                                                                 <  1:99136/140‑1 (MQ=255)                                                          gCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCccg                                                                 >  2:246638/1‑140 (MQ=255)                                                                               gCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGatc                                            >  2:831211/1‑140 (MQ=255)                                                                                   cAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAGCTCCAGCCCGCCATACTATTTTTTGATGATCATcg                                        >  2:265027/1‑140 (MQ=255)                                                                                       tCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCgaa                                    >  1:902154/1‑140 (MQ=255)                                                                                       tCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCgaa                                    >  2:748782/1‑140 (MQ=255)                                                                                             gggCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACg                              <  2:902154/140‑1 (MQ=255)                                                                                             gggCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACg                              <  1:1012059/140‑1 (MQ=255)                                                                                               gCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGAt                            <  1:748782/140‑1 (MQ=255)                                                                                               gCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGAt                            <  1:766964/140‑1 (MQ=255)                                                                                                ccTAATGAGTTTGGCGCGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGATg                           <  1:376902/140‑1 (MQ=255)                                                                                                ccTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGATg                           <  1:197601/140‑1 (MQ=255)                                                                                                    aTGAGTTTGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGATGctct                       >  2:855602/1‑140 (MQ=255)                                                                                                         tttGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGATGCTCTTTAAg                  >  1:818370/1‑140 (MQ=255)                                                                                                         tttGGCACGCCGACGGTAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGATGCTCTTTAAg                  >  2:885901/1‑140 (MQ=255)                                                                                                                         tAATCGCCAGGTTCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGATGCTCTTTAAGGTACGCCCACAACTcc  <  1:246638/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 GTGCAAAGCAGGGGATCTCCTGACCACGCGCCAGACGCGGCAGATAGTGATCTTTCTGCTCGTCAGTGCCGTAATGTTGCAACAGTTCGCCCGGGCCTAATGAGTTTGGCACGCCGACGGTAATCGCCAGGATCCCGCTCACGCCGGAGAGTTTTTGCAGCACGCGAGACTGGGCATAAGCCGAGAACTCCAGCCCGCCGTACTCTTTTTTGATGATCATCGCGAAGAAACGATGCTCTTTAAGGTACGCCCACAACTCC  >  NC_000913/242586‑242845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑