Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,359,279	T→C	T292A (ACC→GCC)	yfaV ←	putative transporter YfaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,359,279	0	T	C	100.0%	108.3 / NA	32	T292A (ACC→GCC)	yfaV	putative transporter YfaV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

CTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGTCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTGAATGGTCAGGTAG  >  NC_000913/2359153‑2359415                                                                                                                               |                                                                                                                                         cTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGcc                                                                                                                             >  1:669373/1‑140 (MQ=255)         gCCATTACTGGAGAAAGCAGCCCGGACAGAGCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCGCAAACAAGGCCGCAACCCa                                                                                                                     >  1:135528/1‑140 (MQ=255)                cTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATg                                                                                                              <  2:255842/140‑1 (MQ=255)                   gagaAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCg                                                                                                           >  2:692266/1‑140 (MQ=255)                      aaaGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTg                                                                                                        <  1:631926/140‑1 (MQ=255)                        agcagcCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGac                                                                                                      >  2:4481/1‑140 (MQ=255)                                      accaataccaatGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTaa                                                                                        >  1:874585/1‑140 (MQ=255)                                       tcaataccaatGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTaaa                                                                                       <  2:1012263/139‑1 (MQ=255)                                               aatGCCCGCCGCCAGTAATGTCAGCACTGTGACATTAGGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACtt                                                                               <  1:368847/140‑1 (MQ=255)                                                atGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGTCGCAACCCGCGGAATGGCGGTGACCGCCGAAGCTGTTAAATCCACatt                                                                              >  2:266260/1‑140 (MQ=255)                                                atGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTa                                                                                         >  2:62704/1‑129 (MQ=255)                                                atGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTa                                                                                         <  1:62704/129‑1 (MQ=255)                                                        cgccAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCTACGGAATGGCGGGGACAACCGACGCCGTAAAACCCACTTTTGTTcccc                                                                      >  2:368847/1‑139 (MQ=255)                                                                       cgcTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACCTTTGTTCCCAGCAATGCCGCAAccc                                                       >  2:970665/1‑139 (MQ=255)                                                                        gcTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTg                                                      >  1:598987/1‑140 (MQ=255)                                                                        gcTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTg                                                      >  2:215532/1‑140 (MQ=255)                                                                           gCGACATTACGCCGTCCGCCGGTTTCATCCGAATAGCACGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGt                                                   <  1:266260/140‑1 (MQ=255)                                                                               cATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGc                                               <  1:960603/140‑1 (MQ=255)                                                                                    cGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGgaa                                          >  2:872221/1‑140 (MQ=255)                                                                                     gCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGgaag                                         >  1:185280/1‑140 (MQ=255)                                                                                      ccGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGgaaaa                                        >  2:830740/1‑138 (MQ=255)                                                                                         ttCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGaaaa                                     >  1:592849/1‑140 (MQ=255)                                                                                           cGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGCCGCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAAtt                                   <  2:135528/140‑1 (MQ=255)                                                                                                         gAATAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGc                     <  1:602765/140‑1 (MQ=255)                                                                                                           aTAGCGCGGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAAccacca                   <  1:692266/140‑1 (MQ=255)                                                                                                               cgcgGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTg               >  1:230375/1‑140 (MQ=255)                                                                                                                 cgGAATAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTGaa             <  2:598987/140‑1 (MQ=255)                                                                                                                     aTAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTGAATGGt         <  1:872221/140‑1 (MQ=255)                                                                                                                     aTAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTGAATGGt         <  2:669373/140‑1 (MQ=255)                                                                                                                     aTAAGCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTGAATGGt         <  2:592849/140‑1 (MQ=255)                                                                                                                         gCCAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTGAATGGTCAgg     <  1:215532/140‑1 (MQ=255)                                                                                                                          ccAGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTGAATGGTCAGGt    <  1:4481/140‑1 (MQ=255)                                                                                                                            aGGCCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTGAACGGTCAGGTAg  <  1:50662/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                         CTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCGTTCGCCGGTTTTATCGGAATAGCGCGGAATAAGCCAGGTCCCAAACAAGGCCGCAACCCACGGAATGGCGGTGACCACCGACGCTGTAAAGCCCACTTTTGTTCCCAGCAATGCCGCAACCTGGGTCGGCAGGAAGAAAATTAATCCGTAAACCGCCACCTGAATGGTCAGGTAG  >  NC_000913/2359153‑2359415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑