Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,429,677	T→C	I188V (ATT→GTT)	purF ←	amidophosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,429,677	0	T	C	100.0%	108.8 / NA	32	I188V (ATT→GTT)	purF	amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

GTAAATCGCTTCGCCCGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTA  >  NC_000913/2429543‑2429803                                                                                                                                       |                                                                                                                               gTAAATCGCTTCGCCCGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGt                                                                                                                           >  2:577013/1‑140 (MQ=255)  tAAATCGCTTCGCCCGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGCTTTACCAGTACCATCGGACGAACCCCGtt                                                                                                                          >  1:479037/1‑140 (MQ=255)         cTTCGCCCGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATcg                                                                                                                   >  1:621854/1‑140 (MQ=255)         cTTCGCCCGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACATTTTCCCAGTAGCATCGGACGAACCCCGTTTGGATcg                                                                                                                   >  1:323809/1‑140 (MQ=255)            cGCCCGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCgg                                                                                                                <  1:577013/140‑1 (MQ=255)            cGCCCGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGCTTTACCAGTACCAGCGCACGAACCCCGTTTGGATCGCgg                                                                                                                >  2:369052/1‑140 (MQ=255)              cccGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGaa                                                                                                              <  2:728367/140‑1 (MQ=255)                cGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAg                                                                                                            <  2:834391/140‑1 (MQ=255)                cGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAg                                                                                                            <  2:621854/140‑1 (MQ=255)                  gcgcGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGaa                                                                                                              >  1:105766/1‑136 (MQ=255)                  gcgcGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGaa                                                                                                              <  2:105766/136‑1 (MQ=255)                              gCAGGAAATCAAAGCTCAGCGTCTCTAGCTCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTgg                                                                                              <  1:469530/140‑1 (MQ=255)                                aGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGcc                                                                                            <  1:612292/140‑1 (MQ=255)                                            cccAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGc                                                                                <  2:816106/140‑1 (MQ=255)                                                                        aaGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAg                                                    <  1:47216/140‑1 (MQ=255)                                                                                 tatatTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTtgtg                                           <  2:867324/140‑1 (MQ=255)                                                                                        tGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACATGCATACGGGCCACGAATTAAGCGGTTTGTGGCAGCaa                                    >  1:355994/1‑140 (MQ=255)                                                                                        tGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCaa                                    >  2:835183/1‑140 (MQ=255)                                                                                        tGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCCCGAATTAAGAGGTTTGTGGCAGCaa                                    >  1:402943/1‑140 (MQ=255)                                                                                          tACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATg                                  >  2:595924/1‑140 (MQ=255)                                                                                           aCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGgc                                         <  2:39595/132‑1 (MQ=255)                                                                                           aCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGgc                                         >  1:39595/1‑132 (MQ=255)                                                                                            cGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCAAACGCGCCACGGGTTGAGCGGTCTGTGGCAGCAATGGc                                >  1:496936/1‑140 (MQ=255)                                                                                             ggTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCa                               >  1:932490/1‑140 (MQ=255)                                                                                              gTTCTCGTCAATATCACGTTTTCCCAGTGCCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAg                              <  1:579015/140‑1 (MQ=255)                                                                                               ttCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGc                             <  2:645597/140‑1 (MQ=255)                                                                                                  tcGTCAATATCACGTTTTCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGaa                          >  2:648618/1‑140 (MQ=255)                                                                                                  tcGTCAATATCACGTTTGCCCAATACCAGCGGACGGACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGaa                          <  2:323809/140‑1 (MQ=255)                                                                                                                ttttCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCtt            <  2:327/140‑1 (MQ=255)                                                                                                                   tCCCAGTACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCa         >  2:1007908/1‑140 (MQ=255)                                                                                                                         tACCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGGAATTGCAGCGAAGATATTGTCGGCTTACAGCGGGt   >  2:463852/1‑140 (MQ=255)                                                                                                                          aCCAGCGGACGAACCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTa  <  1:562667/140‑1 (MQ=255)                                                                                                                          aCCAGCGGACGAACCACGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTa  <  1:463852/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                               GTAAATCGCTTCGCCCGGCGCGACGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTA  >  NC_000913/2429543‑2429803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑