| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,431,651 | T→C | T12A (ACG→GCG) | dedD ← | cell division protein DedD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,431,651 | 0 | T | C | 97.8% | 154.6 / NA | 46 | T12A (ACG→GCG) | dedD | cell division protein DedD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (25/20); minor base A (1/0); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TATCAGGCTCATCACGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGG > NC_000913/2431522‑2431783 | tatCAGGCTCATCACGATCGCCCGCTTTCAGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAAc < 1:359034/140‑1 (MQ=255) cAGGCTCATCACGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGa < 1:765988/140‑1 (MQ=255) aGGCTCATCACGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGAt > 2:125538/1‑140 (MQ=255) ggCTCATCACGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGAtt > 1:931195/1‑140 (MQ=255) ggCTCATCACGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAACACGATCGCGCCCACTAACCGAtt > 2:588874/1‑140 (MQ=255) catcaCGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGa > 2:138747/1‑140 (MQ=255) catcaCGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAACCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGa > 2:938489/1‑140 (MQ=255) atcaCGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGTCACGAGCACGATCGCGCCAACTAACCGATTCTGaa > 2:461028/1‑140 (MQ=255) tcaCGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAAc < 2:986893/129‑1 (MQ=255) tcaCGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAAc > 1:986893/1‑129 (MQ=255) tcaCGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGaaa > 1:713730/1‑140 (MQ=255) caCGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAac < 2:628773/140‑1 (MQ=255) caCGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAac > 1:18316/1‑140 (MQ=255) caCGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAac < 1:125538/140‑1 (MQ=255) tCGCCCGCTTTCGGCACTAGCGGGATAGCCGAGAATTCACCCTGATAACGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAacttact < 2:265309/140‑1 (MQ=255) cccGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGc > 1:678863/1‑140 (MQ=255) ccGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGAGCCACCAGCACGATCGAGCCCACTAACCGAATCTGAAACTTACTTGcc > 2:357235/1‑140 (MQ=255) ttCGGCACCAGCGGGATCGCCGGGAACTCATCCTGGTAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGc < 2:399861/140‑1 (MQ=255) ccAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTctcct < 2:678863/140‑1 (MQ=255) aGCGGGATAGCCGCGAACTCATCCTGATAATTTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTTACCGATTATGATACGTACTTGTCACCGCTTctcctcc > 1:481477/1‑139 (MQ=255) aGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTcg > 2:252375/1‑140 (MQ=255) cGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTcgcg > 1:772452/1‑140 (MQ=255) tAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAAt < 1:252375/140‑1 (MQ=255) aGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATc < 1:138747/140‑1 (MQ=255) gCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCa < 1:728498/140‑1 (MQ=255) gcgAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAGACTTTCTTGCCCCCCCTTCTCTTCGCGTCAATCACtt > 1:351813/1‑140 (MQ=255) gcgAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACtt > 1:151550/1‑140 (MQ=255) aaCTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCa > 1:931823/1‑140 (MQ=255) aaCTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCa > 1:773991/1‑140 (MQ=255) cTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATg < 2:151550/140‑1 (MQ=255) cTCATCCTGATAATGTTGTTTCAGCCCGTCCTGCATCCCGGGAAGTACTATCGCCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTTCTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATg < 2:351813/140‑1 (MQ=255) cATCCTGATAATGTTATTTCTGCCCGTCCTGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGAc < 1:357235/140‑1 (MQ=255) tAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAACACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGa < 1:588874/140‑1 (MQ=255) aaTGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGAc < 1:461028/140‑1 (MQ=255) aTGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGAcc < 2:728468/140‑1 (MQ=255) gTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACca < 2:931195/140‑1 (MQ=255) tCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACcac > 1:74760/1‑140 (MQ=255) agcagcCCTGAAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACcacaca < 2:226210/140‑1 (MQ=255) gAAGTACAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACAGGTGCGACGGTGGGGGAAGAACTACACACCAGCACGgt > 1:265428/1‑140 (MQ=255) aaGTACAATCACCCCTAGCGCCTCCTGCACGATCGCGTCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGgtg < 1:271180/140‑1 (MQ=255) aCAATCACCCCCAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCtt > 1:66042/1‑140 (MQ=255) cccAGCGCCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAg > 1:599769/1‑140 (MQ=255) cgcCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTcc > 1:231463/1‑140 (MQ=255) cgcCACCAGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTcc > 1:975505/1‑140 (MQ=255) accaGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCGCACACCAACACGGTGTCTTCCGCTTTAGCGTCCGCCa > 1:380228/1‑140 (MQ=255) accaGCACGATCGCGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCa > 1:6160/1‑140 (MQ=255) aCGATCGCGCCCACTACACGATCCTGAAACCAAATGGCCACCGCTTCTACTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCgg < 2:481477/140‑1 (MQ=255) | TATCAGGCTCATCACGATCGCCCGCTTTCGGCACCAGCGGGATAGCCGCGAACTCATCCTGATAATGTTTTTTCTGCCCGTCCAGCAGCCCTGGAAGTACAATCACCCCCAGCGCCACCAGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGG > NC_000913/2431522‑2431783 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |