Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,501,698	T→C	V190A (GTT→GCT)	ypdC →	putative AraC‑type DNA‑binding transcriptional regulator YpdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,501,698	0	T	C	100.0%	191.3 / NA	57	V190A (GTT→GCT)	ypdC	putative AraC‑type DNA‑binding transcriptional regulator YpdC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/32);  total (25/32)

GTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGTTTTATCGAGTATGTGCGTTGGGTGCGAATG  >  NC_000913/2501563‑2501834                                                                                                                                        |                                                                                                                                         gTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAg                                                                                                                                      <  1:915857/140‑1 (MQ=255)  ttCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAgg                                                                                                                                     >  2:905278/1‑140 (MQ=255)  ttCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAgg                                                                                                                                     >  2:834264/1‑140 (MQ=255)   tCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGa                                                                                                                                    <  2:697195/140‑1 (MQ=255)   tCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGa                                                                                                                                    <  2:843923/140‑1 (MQ=255)    cgccgcTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGGGATATTCTCTATCACAACATTAGTAACTGGGCTCAGGAt                                                                                                                                   >  2:337546/1‑140 (MQ=255)    cgccgcTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCACAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGAt                                                                                                                                   >  1:243885/1‑140 (MQ=255)              gCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGccc                                                                                                                         <  2:655236/140‑1 (MQ=255)                    tatCATTCAGCCGCTTGTGTTAAGTCTGCCGCATCTTGGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGc                                                                                                                   <  1:359447/140‑1 (MQ=255)                    tatCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGc                                                                                                                   <  2:246541/140‑1 (MQ=255)                       cATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCa                                                                                                                <  1:158187/140‑1 (MQ=255)                          tCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCAccc                                                                                                             >  2:1010597/1‑140 (MQ=255)                            aGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCcgc                                                                                                           >  2:957238/1‑140 (MQ=255)                              ccGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCGAGCCGCTCACCCGCga                                                                                                         >  2:439049/1‑140 (MQ=255)                              ccGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCga                                                                                                         >  2:509868/1‑140 (MQ=255)                                 cTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGTCGCTCACCCGCGagag                                                                                                      >  2:79409/1‑140 (MQ=255)                                   ggTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCg                                                                                                    >  1:550404/1‑140 (MQ=255)                                   ggTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCGCAGGATAGTTATGCGCAGCCGGTCACCCGCGAGAGCg                                                                                                    >  1:317632/1‑140 (MQ=255)                                   ggTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTGGTAACTGGCCTCCGGATAATTATGCCCAGCAGCTCACCCGCGAGAGCg                                                                                                    >  2:320093/1‑140 (MQ=255)                                        tAAGTTTGCTGCATCTTTGCCGTAGTGTGTTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGgcg                                                                                               <  1:376935/140‑1 (MQ=255)                                        tAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGgcg                                                                                               <  1:607971/140‑1 (MQ=255)                                        tAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGgcg                                                                                               <  1:542257/140‑1 (MQ=255)                                        tAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGgcg                                                                                               <  1:725438/140‑1 (MQ=255)                                        tAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGgcg                                                                                               <  1:509868/140‑1 (MQ=255)                                        tAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGgcg                                                                                               <  1:50938/140‑1 (MQ=255)                                           gTTTGCTGCATCTTTACCGTAGTGTAGTGTATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAg                                                                                            <  1:429946/140‑1 (MQ=255)                                              tgctgcATCATTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGttt                                                                                         >  2:625306/1‑140 (MQ=255)                                                 tgcATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGtttttt                                                                                      >  1:619067/1‑140 (MQ=255)                                                     tCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGgcgc                                                                                              <  1:826850/128‑1 (MQ=255)                                                     tCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGgcgc                                                                                              >  2:826850/1‑128 (MQ=255)                                                       tttGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGTCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATc                                                                                <  1:79409/140‑1 (MQ=255)                                                             gTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCAACCGCGAGTGCGGGGTGCAGTTTTTTAATATCACGccc                                                                          >  1:465738/1‑140 (MQ=255)                                                                  gtggtgAATATGCCGCCGGGCAGTTCGCAGCCGAGCGGCTATTTTCTCGATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAatca                                                                     <  2:465738/140‑1 (MQ=255)                                                                          tatGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTCGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCaa                                                             >  1:796374/1‑140 (MQ=255)                                                                              ccgccgGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACt                                                         <  1:615925/140‑1 (MQ=255)                                                                               cgccgGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTg                                                        >  1:896222/1‑140 (MQ=255)                                                                                gccgGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGt                                                       >  2:640069/1‑140 (MQ=255)                                                                                gccgGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGt                                                       >  1:815327/1‑140 (MQ=255)                                                                                   gggCAATTCGCAGCCTCGCGGCAATTTTCCCTATCACTGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTg                                                    <  1:320093/140‑1 (MQ=255)                                                                                     gCAATTCGCAGCCGCGCGGCGATTTGCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCt                                                  <  1:337546/140‑1 (MQ=255)                                                                                     gCAATTCGCAGCCGCCCGGCGAATTCCTCTATCACAGGATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCt                                                  <  1:367220/140‑1 (MQ=255)                                                                                     gCAATTCACAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCt                                                  <  2:243885/140‑1 (MQ=255)                                                                                             cAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACGGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATgg                                          <  2:345789/140‑1 (MQ=255)                                                                                              aGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGa                                         <  1:957238/140‑1 (MQ=255)                                                                                                 cgcgcgGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTATAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACg                                      >  2:695672/1‑140 (MQ=255)                                                                                                   cgcgGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAGAAATGTTTGCTCAGCATGGAACGAt                                    >  1:332762/1‑140 (MQ=255)                                                                                                   cgcgGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGAt                                    <  2:896222/140‑1 (MQ=255)                                                                                                   cgcgGCGATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGAt                                    >  1:192490/1‑140 (MQ=255)                                                                                                         gATTTTCTCTATCACAGTATTTTTAACTGGGCTCAGGATTATTATGCCTAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGttt                              <  2:317632/140‑1 (MQ=255)                                                                                                         gATTTTCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGttt                              <  2:550404/140‑1 (MQ=255)                                                                                                           ttttCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGTTTTa                            <  1:1010597/140‑1 (MQ=255)                                                                                                           ttttCTCTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGTTTTa                            <  1:625306/140‑1 (MQ=255)                                                                                                              tctcTATCACAGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGTTTTATCg                         <  1:905278/140‑1 (MQ=255)                                                                                                                        aGCATTTGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGTTTTATCGAGTATGTGCg               <  1:640069/140‑1 (MQ=255)                                                                                                                         agaTTTGTAACTGGGCTTAGGAAAATTATGCCCAGCCGCGCACCCGCGGGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGTTTTATCGAGTATGTGCGt              <  2:433783/138‑1 (MQ=255)                                                                                                                            tttGTAACTGGGCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGTTTTATCGAGTATGTGCGTTgg           >  2:550660/1‑140 (MQ=255)                                                                                                                                     gggCTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGTTTTATCGAGTATGTGCGTTGGGTGCGAATg  >  1:11061/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         GTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGCCCAATCATCTGTCAAAACTGTTTGCTCAGCATGGAACGATGCGTTTTATCGAGTATGTGCGTTGGGTGCGAATG  >  NC_000913/2501563‑2501834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑