Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,502,375	G→A	A704V (GCG→GTG)	fryA ←	putative PTS multiphosphoryl transfer protein FryA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,502,375	0	G	A	100.0%	145.9 / NA	43	A704V (GCG→GTG)	fryA	putative PTS multiphosphoryl transfer protein FryA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/20);  total (23/20)

ATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCC  >  NC_000913/2502238‑2502506                                                                                                                                          |                                                                                                                                    aTTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCAcc                                                                                                                                   >  2:111221/1‑140 (MQ=255)   ttGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTg                                                                                                                                 >  1:1020253/1‑140 (MQ=255)      gtgtGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGctc                                                                                                                              >  2:824714/1‑140 (MQ=255)         tgCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCttt                                                                                                                           >  2:194522/1‑140 (MQ=255)         tgCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCACTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCttt                                                                                                                           >  2:849225/1‑140 (MQ=255)          gCGGGATCGCTACGCCAAAACCCACCCCGCTGGTAACTATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTa                                                                                                                          <  1:259209/140‑1 (MQ=255)           cGGGATCGCTACGCCCAAACCACCGACGGCGGTAACACTCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTAt                                                                                                                         <  1:282497/140‑1 (MQ=255)           cGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTAt                                                                                                                         <  1:214839/140‑1 (MQ=255)           cGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTAt                                                                                                                         <  2:613229/140‑1 (MQ=255)           cGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCAGCAGAGAAACTGGGTCACCTGCTCTTTAt                                                                                                                         >  1:294076/1‑140 (MQ=255)               aTCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCt                                                                                                                     <  2:857086/140‑1 (MQ=255)                   cTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGTTGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTaaaa                                                                                                                 >  2:668983/1‑140 (MQ=255)                          aaaaCCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGAt                                                                                                          <  1:898831/140‑1 (MQ=255)                          aaaaCCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGAt                                                                                                          <  1:250213/140‑1 (MQ=255)                                aaCGCCGGTGGTAACAAACTCTTCCCGCTGCCAGACATCTTCTTCCAGGTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATcaacaa                                                                                                    <  2:434345/140‑1 (MQ=255)                                  cGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAg                                                                                                  >  2:89316/1‑140 (MQ=255)                                       gtggtAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATAtt                                                                                             >  2:907180/1‑140 (MQ=255)                                           tAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTcc                                                                                         <  2:34899/140‑1 (MQ=255)                                           tAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTcc                                                                                         <  1:864285/140‑1 (MQ=255)                                                    ctTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCcagc                                                                                >  2:140555/1‑140 (MQ=255)                                                          gCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTggg                                                                          <  1:140555/140‑1 (MQ=255)                                                              ccAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGaa                                                                      <  1:576340/140‑1 (MQ=255)                                                                        tcttcCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCttcttc                                                            >  1:697196/1‑140 (MQ=255)                                                                         cttcCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGTTTTCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTcc                                                           >  2:520858/1‑140 (MQ=255)                                                                                cTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTaa                                                    >  2:147245/1‑140 (MQ=255)                                                                                cTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTaa                                                    >  2:503981/1‑140 (MQ=255)                                                                                cTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTaa                                                    >  1:612720/1‑140 (MQ=255)                                                                                   aaaCGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAgg                                                 <  2:697196/140‑1 (MQ=255)                                                                                               aGTGCGCCCGTTAACGCGGAGGTTGCCGCACAGGAGCTGTATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACg                                     <  2:419620/140‑1 (MQ=255)                                                                                                  gcgcCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCtt                                  >  2:104196/1‑140 (MQ=255)                                                                                                   cgcCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTc                                 >  2:625572/1‑140 (MQ=255)                                                                                                   cgcCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTc                                 >  2:671370/1‑140 (MQ=255)                                                                                                   cgcCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACATCTGCTTCCGGCATAAACGCGGCGAGTAACGCTTc                                 >  2:269593/1‑140 (MQ=255)                                                                                                      ccGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGGCTTCTTCGGGCGTATAGGCGGTGAGTAACGCTTCAAt                              >  1:419620/1‑140 (MQ=255)                                                                                                       cGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGTGGTGAGTAACGCTTCAAtt                             <  1:824714/140‑1 (MQ=255)                                                                                                         ttAACGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTCCTTCCGGTGTATAGCCGGTGAATAACGCTTCAATTTc                           >  1:346426/1‑140 (MQ=255)                                                                                                           aaCGCCGAGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCt                         <  2:233544/140‑1 (MQ=255)                                                                                                                  aGGTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACt                  >  2:707060/1‑140 (MQ=255)                                                                                                                   ggTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTg                 <  1:89316/140‑1 (MQ=255)                                                                                                                    gTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGc                >  1:618850/1‑140 (MQ=255)                                                                                                                    gTTGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGGAAAGCTTCAATTTCCTGCGCACTGc                >  1:379251/1‑140 (MQ=255)                                                                                                                     ttGCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCg               <  1:671370/140‑1 (MQ=255)                                                                                                                       gCCGCACAGGAACTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGc             <  1:104196/140‑1 (MQ=255)                                                                                                                                  aCTGGATCACCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGcc  <  2:678531/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    ATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCC  >  NC_000913/2502238‑2502506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑