Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,596,881	A→G	intergenic (‑144/+24)	ypfJ ← / ← purC	uncharacterized protein YpfJ/phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,596,881	0	A	G	100.0%	236.8 / NA	68	intergenic (‑144/+24)	ypfJ/purC	uncharacterized protein YpfJ/phosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/22);  total (46/22)

CTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTT  >  NC_000913/2596744‑2597012                                                                                                                                          |                                                                                                                                    cTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGcgcg                                                                                                                                   >  1:61240/1‑140 (MQ=255)  tCAACAATATATTCATTCTAGATATGATCGTCGGTCGTTCAGAGGAAGTTTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCgg                                                                                                                                  <  2:280462/140‑1 (MQ=255)   caacaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATAGTCCGAGAGCGGGTGTCTTGCCGTGCCAGCTGCGCGGc                                                                                                                                 >  1:280462/1‑140 (MQ=255)     acaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGTGGTTGTCTTGCCGTGCCAGCTGCGCGGCaa                                                                                                                               >  2:233336/1‑140 (MQ=255)      caaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAg                                                                                                                              >  1:629441/1‑140 (MQ=255)           tatTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAgatgat                                                                                                                         >  1:885158/1‑140 (MQ=255)              tCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATAATCCGAGAGCGGTGGCCTTGCCGTGCCAGCTTCGCGGCAAGATGATGaa                                                                                                                      >  2:451633/1‑140 (MQ=255)                 ttATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAg                                                                                                                   >  2:88743/1‑140 (MQ=255)                   ataGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGaa                                                                                                                 >  1:597829/1‑140 (MQ=255)                   ataGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGaa                                                                                                                 >  1:46568/1‑140 (MQ=255)                   ataGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGaa                                                                                                                 >  1:944040/1‑140 (MQ=255)                     aGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGaaaa                                                                                                               >  2:863840/1‑140 (MQ=255)                       aaaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGAtgatga                                                                                                                       >  1:654867/1‑130 (MQ=255)                       aaaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGAtgatga                                                                                                                       <  2:654867/130‑1 (MQ=255)                       aaaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAAt                                                                                                             <  1:863840/140‑1 (MQ=255)                            aTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAg                                                                                                          <  1:943930/138‑1 (MQ=255)                            aTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAg                                                                                                          >  2:943930/1‑138 (MQ=255)                            aTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTc                                                                                                        >  1:546560/1‑140 (MQ=255)                            aTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTc                                                                                                        <  2:629441/140‑1 (MQ=255)                            aTCGTAGGTAGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTc                                                                                                        >  1:734707/1‑140 (MQ=255)                             tCGTAGGGTGTTTAGTGGAAGATTTCCACTGGAAAGCCGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTcc                                                                                                       <  1:490293/140‑1 (MQ=255)                                      gtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTaca                                                                                              >  2:796960/1‑140 (MQ=255)                                       ttCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGTTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTacac                                                                                             >  2:640036/1‑140 (MQ=255)                                       ttCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTcc                                                                                                       <  1:68209/130‑1 (MQ=255)                                       ttCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTcc                                                                                                       >  2:68209/1‑130 (MQ=255)                                       ttCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTacac                                                                                             >  2:722412/1‑140 (MQ=255)                                                 gATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGgcggcg                                                                                   >  2:43466/1‑140 (MQ=255)                                                 gATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGgcggcg                                                                                   >  1:862099/1‑140 (MQ=255)                                                 gATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATCAACAGAAAAATCAGTCCAGCTGTACACCCAGgcggcg                                                                                   >  1:389482/1‑140 (MQ=255)                                                 gATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGCCAAGATGATGAACAGAATAATTAGTCCAGCTGTACACCCAGgcggcg                                                                                   >  1:393255/1‑140 (MQ=255)                                                      ccACAGGAAAGCGGGTTGAAATGGCTGAGCAATGAGCTTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGa                                                                              <  1:489851/140‑1 (MQ=255)                                                      ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGa                                                                              >  1:151758/1‑140 (MQ=255)                                                      ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGa                                                                              >  1:569825/1‑140 (MQ=255)                                                      ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGa                                                                              >  1:101801/1‑140 (MQ=255)                                                       cacaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGAc                                                                             <  2:569825/140‑1 (MQ=255)                                                        acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACg                                                                            <  1:451633/140‑1 (MQ=255)                                                        acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGAAGATGAACAAAAAAATCCGTCCAGCTGTACAACCAGGCGGCGGGCGACg                                                                            >  2:392595/1‑140 (MQ=255)                                                          aGGAAAGCGGGATGAAATGGCGGAGGAATTAGCGTTCGATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGc                                                                          <  1:392595/140‑1 (MQ=255)                                                          aGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGc                                                                          <  2:734707/140‑1 (MQ=255)                                                           ggAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAATTCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCt                                                                         >  1:348282/1‑140 (MQ=255)                                                              aaGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGATCAGAAAAAACAGACCAGCTGTACACTCAGGAGGCGGGCGACGGCTTCa                                                                      >  2:375587/1‑140 (MQ=255)                                                              aaGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCa                                                                      >  1:210281/1‑140 (MQ=255)                                                               aGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCAt                                                                     >  2:94828/1‑140 (MQ=255)                                                                 cGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAg                                                                   >  1:159807/1‑140 (MQ=255)                                                                 cGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAg                                                                   >  2:708474/1‑140 (MQ=255)                                                                    gATGAAATGGCGGAGGCATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCt                                                                <  2:619944/140‑1 (MQ=255)                                                                    gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCATCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCt                                                                >  1:460539/1‑140 (MQ=255)                                                                    gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCt                                                                <  2:885158/140‑1 (MQ=255)                                                                         aaTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGAt                                                           <  2:210281/140‑1 (MQ=255)                                                                              cGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGc                                                      >  2:20511/1‑140 (MQ=255)                                                                                        aGCGTTCAATGCATATATTCCGGGATCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGct                                            <  1:367849/140‑1 (MQ=255)                                                                                        aGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCCACGACTTCATAGCCTTCCATCAGGCCACCGAGGct                                            >  2:367849/1‑140 (MQ=255)                                                                                                 tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGaa                                   >  2:983074/1‑140 (MQ=255)                                                                                                  gCATATATTCCGAGAGCAGTTGTCTTGCCGTGCCAGCTGCGCGGAAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGaaa                                  <  1:635286/140‑1 (MQ=255)                                                                                                      atatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGt                              >  2:211226/1‑140 (MQ=255)                                                                                                       tatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTc                             <  2:151758/140‑1 (MQ=255)                                                                                                        atTCCGAGAGCGGTTTTCTTGCTGTGCCACCTGCGCGCCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCt                            <  1:456505/140‑1 (MQ=255)                                                                                                        atTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCt                            <  1:983074/140‑1 (MQ=255)                                                                                                              agagCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCTATCAGTCGACCGAGGCTCTGGGGGAAACGGACTTTGTcc                      >  2:456505/1‑140 (MQ=255)                                                                                                              agagCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTcc                      >  2:981868/1‑140 (MQ=255)                                                                                                                agCGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCGGGCCACCCAGGATCTGGCGGAAACGGTCTTTGTCCAt                    >  1:500939/1‑140 (MQ=255)                                                                                                                  cGGTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCAttt                  >  2:94347/1‑140 (MQ=255)                                                                                                                   ggTTGTCTTGCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGATTCGATCACGCCACAGAGGCTCTGGCGGAAAAGGTCTTTGTCCAtttt                 >  2:475561/1‑140 (MQ=255)                                                                                                                       gTCTTGCCGTGCCAGATGCGCGACACGATCATGAACAAAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTcc             <  1:375587/140‑1 (MQ=255)                                                                                                                            gCCGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGt        >  2:54280/1‑140 (MQ=255)                                                                                                                             ccGTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCAAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGtt       >  2:596860/1‑140 (MQ=255)                                                                                                                               gTGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTc     >  1:893861/1‑140 (MQ=255)                                                                                                                                tGCCAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCt    <  2:159807/140‑1 (MQ=255)                                                                                                                                  ccAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCttt  >  1:531188/1‑140 (MQ=255)                                                                                                                                  ccAGCTGCGCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCttt  >  2:851276/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    CTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTT  >  NC_000913/2596744‑2597012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑