Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,940	T→C	intergenic (‑194/+129)	kgtP ← / ← rrfG	alpha‑ketoglutarate:H(+) symporter/5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,940	0	T	C	100.0%	195.2 / NA	56	intergenic (‑194/+129)	kgtP/rrfG	alpha‑ketoglutarate:H(+) symporter/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/24);  total (32/24)

AAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCC  >  NC_000913/2725809‑2726073                                                                                                                                    |                                                                                                                                      aaaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAg                                                                                                                               <  1:655157/140‑1 (MQ=255)         tattatACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATcc                                                                                                                       <  1:69094/140‑1 (MQ=255)              taCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGc                                                                                                                  <  2:157697/140‑1 (MQ=255)                 aTTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGa                                                                                                               >  1:1019865/1‑140 (MQ=255)                   ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAAt                                                                                                             <  2:535184/140‑1 (MQ=255)                   ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAAt                                                                                                             >  2:190793/1‑140 (MQ=255)                   ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAAt                                                                                                             >  2:526615/1‑140 (MQ=255)                   ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAAt                                                                                                             >  2:744575/1‑140 (MQ=255)                   ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAAt                                                                                                             >  1:389155/1‑140 (MQ=255)                   ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAAt                                                                                                             >  1:232016/1‑140 (MQ=255)                   ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGCGAAGCCGTATCCGTTGCTGAAt                                                                                                             >  1:421119/1‑140 (MQ=255)                   ttgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGCACACGTATCGAAATGGTCAAGCTGGGGAAGTCGTATCCGATGCTGAAt                                                                                                             >  2:466390/1‑140 (MQ=255)                   ttgttgCATATCATTATGCAACCTTAACCATAAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAAt                                                                                                             >  2:563591/1‑140 (MQ=255)                    tgttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATc                                                                                                            >  2:189122/1‑140 (MQ=255)                      ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTg                                                                                                          <  2:944256/140‑1 (MQ=255)                      ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTg                                                                                                          <  1:911587/140‑1 (MQ=255)                           tatCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCata                                                                                                     >  1:718800/1‑140 (MQ=255)                             tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGGGCTTAAGGTTAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGAAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAtg                                                                                                   <  2:667831/140‑1 (MQ=255)                             tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAtg                                                                                                   <  1:526615/140‑1 (MQ=255)                               aTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAtgtg                                                                                                 <  1:189122/140‑1 (MQ=255)                               aTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAtgtg                                                                                                 <  2:1019865/140‑1 (MQ=255)                               aTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCAGGGGAAGACGTATCTGTTGCTGAATCTGGCATAtgtg                                                                                                 >  1:401332/1‑140 (MQ=255)                                ttATGCAACCTTAACCAAGAATTTAGTTAGCCGGAAAATTGTTATTGACGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                                                                                                <  2:401332/140‑1 (MQ=255)                                 tATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTggg                                                                                               <  2:754660/140‑1 (MQ=255)                                 tATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTggg                                                                                               >  1:209452/1‑140 (MQ=255)                                        ccTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGGGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATaa                                                                                        >  2:589947/1‑140 (MQ=255)                                        ccTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGc                                                                                                        >  2:506932/1‑124 (MQ=255)                                        ccTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGc                                                                                                        <  1:506932/124‑1 (MQ=255)                                        ccTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATaa                                                                                        >  1:596552/1‑140 (MQ=255)                                           tAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGAc                                                                                     >  1:558215/1‑140 (MQ=255)                                             aCCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGTAGTATAAGAcgc                                                                                   >  1:1008566/1‑140 (MQ=255)                                             aCCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGAcgc                                                                                   >  1:908914/1‑140 (MQ=255)                                             aCCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGAcgc                                                                                   >  2:837491/1‑140 (MQ=255)                                                  gAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCAGGCTTACGTGGGAGTATAAGACGCGCAGc                                                                              >  1:339431/1‑140 (MQ=255)                                                  gAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGACGTATCCGGTGCTGAATCTGGCATAAGTGGGAGTATAAGACGCGCAGc                                                                              >  2:300408/1‑140 (MQ=255)                                                       tagttagCAGGAAAATGTTTATTGAGGAGCTGAAGTATAAATTTCTGGCAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGc                                                                         <  2:339431/140‑1 (MQ=255)                                                            agCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGAcgcg                                                                                  >  2:961989/1‑126 (MQ=255)                                                            agCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGAcgcg                                                                                  <  1:961989/126‑1 (MQ=255)                                                                           aTTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTgcg                                                     >  1:1012195/1‑140 (MQ=255)                                                                           aTTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTgcg                                                     >  1:838970/1‑140 (MQ=255)                                                                                 gagCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGTCGCCCAGTGTCGCATCCGGCATTTTTTTCTGCGCCAATg                                               >  2:369911/1‑140 (MQ=39)                                                                                    cTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCaa                                            >  1:919109/1‑140 (MQ=255)                                                                                     ttAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCaaa                                           <  1:221796/140‑1 (MQ=255)                                                                                          gATAAATTTCTGGTAAGTAGGACACGTATGGTAGTTGGCCAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGc                                      <  2:389155/140‑1 (MQ=38)                                                                                          gATAAATTTCTGGTAAGGAGGACACGTATGGTAGTGGGCCAGCTGGGGGAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGc                                      <  2:421119/140‑1 (MQ=255)                                                                                            tAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCa                                    <  2:838970/140‑1 (MQ=255)                                                                                                 ttCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGt                               <  2:718800/140‑1 (MQ=255)                                                                                                          ggaggaCACGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGc                      >  2:921767/1‑140 (MQ=255)                                                                                                                  cGTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCa              <  1:190793/140‑1 (MQ=255)                                                                                                                   gTATGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCAt             <  2:596552/140‑1 (MQ=255)                                                                                                                      tGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCa              >  2:22020/1‑136 (MQ=255)                                                                                                                      tGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCa              <  1:22020/136‑1 (MQ=255)                                                                                                                      tGGAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAAt          >  1:143298/1‑140 (MQ=255)                                                                                                                       ggAAGTGGGCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAAtt         <  2:232016/140‑1 (MQ=255)                                                                                                                              ggCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGcc  >  2:616935/1‑140 (MQ=255)                                                                                                                              ggCAAGCTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGcc  >  1:206441/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      AAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCC  >  NC_000913/2725809‑2726073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑