Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,731,168	T→C	intergenic (‑11/+432)	rrsG ← / ← clpB	16S ribosomal RNA/ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,731,168	0	T	C	100.0%	71.6 / NA	30	intergenic (‑11/+432)	rrsG/clpB	16S ribosomal RNA/ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/19);  total (11/19)

GACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCA  >  NC_000913/2731034‑2731206                                                                                                                                       |                                       gACATTTCTCACCCGACCGCCAATCCTCATCGAAGCATCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCa                                   <  1:375460/140‑1 (MQ=11) gACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCa                                   <  1:807945/140‑1 (MQ=18) gACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCa                                   <  1:729972/140‑1 (MQ=18) gACATTACTCACCCGTCCGCCAATCGTCAGCGAAGCAGCCAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCa                                   <  2:389601/140‑1 (MQ=255)       aCTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAAt                             >  2:924516/1‑140 (MQ=21)           aCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCTAACGCATCAATTTAAACGTTTGACGCTCAATGAATTaaa                         >  2:452468/1‑140 (MQ=11)             ccGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACt                       >  1:610305/1‑140 (MQ=37)              cGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTTTTCAATTTAAACGTATGACGCACAAAGAATTAAACtt                      >  1:365108/1‑140 (MQ=11)              cGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACtt                      >  1:909587/1‑140 (MQ=37)                tCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCg                    >  1:673283/1‑140 (MQ=37)                tCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCg                    >  1:34719/1‑140 (MQ=37)                tCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCg                    >  2:230974/1‑140 (MQ=21)                tCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTCGACGCTCAAAGAATTAAACTTCg                    >  1:722800/1‑140 (MQ=255)                tCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGAGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCg                    >  1:167897/1‑140 (MQ=255)                 ccGCCACTCGTCAGCGACGCAGCAAGCTGCTCCCTTTTACCGTTCGACCTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAGGTTTGACGCTCAAAGAATTAAACTTCGt                   <  2:265519/140‑1 (MQ=11)                 ccGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCATCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGt                   <  2:755021/140‑1 (MQ=16)                 ccGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGt                   <  1:733167/140‑1 (MQ=18)                 ccGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGt                   <  2:175814/140‑1 (MQ=18)                     cACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCATACTCTTCACTTTAAAACTTTGACGCTCAAAGAATTAAACTTCGTAATg               >  2:286724/1‑140 (MQ=255)                         cGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGTAATGAAtt           <  2:617173/140‑1 (MQ=21)                          gTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGTAATGAATTa          <  1:782763/140‑1 (MQ=18)                          gTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGTAATGAATTa          <  1:240638/140‑1 (MQ=18)                           tCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGTAATGAATTAc         <  1:184155/140‑1 (MQ=21)                                gAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGTAATGAATTACGTGtt    <  2:673283/140‑1 (MQ=17)                                gAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGTAATGAATTACGTGtt    <  2:814606/140‑1 (MQ=18)                                 aaGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTc   <  2:603712/140‑1 (MQ=14)                                  agcagcAAGCTGCTTCCTGTTATCGTTCGACTTGCCTGTTTTAGGCCTCCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCa  <  2:365108/140‑1 (MQ=11)                                  agcagcAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTTGTAATGAATTACGTGTTCa  <  1:767603/140‑1 (MQ=9)                                  agcagcAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCa  <  1:230974/140‑1 (MQ=14)                                  agcagcAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTCGACGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCa  <  2:722800/140‑1 (MQ=9)                                                                                                                                       |                                       GACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCA  >  NC_000913/2731034‑2731206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑