Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,767,482	T→C	intergenic (+127/‑228)	rnlB → / → yfjP	CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,767,482	0	T	C	100.0%	179.8 / NA	56	intergenic (+127/‑228)	rnlB/yfjP	CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/25);  total (31/25)

TGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAA  >  NC_000913/2767353‑2767614                                                                                                                                  |                                                                                                                                     tGAAACTCTTCTTTCTGAACGACCCAGTATGCAGTGCGCTCTTTTGGCGTGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGa                                                                                                                            <  2:499012/140‑1 (MQ=255) tGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTTCGGCATATGGACAAGGTTTTCCCCTGCGCACCCACgc                                                                                                                            >  1:455703/1‑139 (MQ=255)   aaaCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACt                                                                                                                          >  2:231683/1‑140 (MQ=255)            tttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTACATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTccc                                                                                                                 <  1:721379/140‑1 (MQ=255)             ttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTcccc                                                                                                                <  2:19664/140‑1 (MQ=255)                   aCGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCttt                                                                                                          >  2:991072/1‑140 (MQ=255)                   aCGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTACACCCGCGCACCCACGACAAAATTTCGCCCCCttt                                                                                                          >  2:475450/1‑140 (MQ=255)                        ccAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACt                                                                                                     <  1:991072/140‑1 (MQ=255)                                 aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCaataa                                                                                            >  2:13837/1‑140 (MQ=255)                                   ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACACACCACTAAATTTCCCCCCCTATAAACTTCTCAATAATg                                                                                          >  2:354306/1‑140 (MQ=255)                                      gcctttttGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCa                                                                                       >  2:964963/5‑140 (MQ=255)                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCAGGACAAAAATTCCCCCCGTTTAGACTTCTCAATAATGTCa                                                                                       >  2:281610/1‑140 (MQ=255)                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCa                                                                                       >  2:512890/1‑140 (MQ=255)                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCa                                                                                       >  2:518967/1‑140 (MQ=255)                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCa                                                                                       >  2:947047/1‑140 (MQ=255)                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCa                                                                                       >  2:243662/1‑140 (MQ=255)                                      gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGCGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCa                                                                                       >  1:778454/1‑140 (MQ=255)                                                  tGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg                                                                           <  1:13837/140‑1 (MQ=255)                                                  ggATATTAACTTGTCCAATATGTTCGAATTGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg                                                                           <  1:459152/139‑1 (MQ=255)                                                       ttAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATc                                                                      <  2:99887/140‑1 (MQ=255)                                                         aaCTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCt                                                                    >  1:653443/1‑140 (MQ=255)                                                           cTTGTCCAATATGATGAAATAGTATGAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTat                                                                  <  1:475450/140‑1 (MQ=255)                                                            ttGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatg                                                                 <  1:947047/140‑1 (MQ=255)                                                              gTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat                                                               >  2:141732/1‑140 (MQ=255)                                                              gTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCACAACAATGTCACGAATTATCTGGAAATCCTatgac                                                               >  2:459152/1‑139 (MQ=255)                                                               tCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                                                              >  2:569550/1‑140 (MQ=255)                                                               tCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                                                              >  1:981815/1‑140 (MQ=255)                                                               tCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                                                              >  1:656323/1‑140 (MQ=255)                                                               tCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTATCAATAACGTCTCCAATTTTCAGGAAATCCTatgatg                                                              >  1:434165/1‑140 (MQ=255)                                                               tCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTATCAATAAAGTCGCCAATTTTCTGGAAATCCTatgatg                                                              >  1:427562/1‑140 (MQ=255)                                                               tCCAATATGATCAAATAGCATTAACAGCCCCTCAAAACATCCTGCAGAGAGAACCCGTTTACCACTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                                                              >  1:590306/1‑140 (MQ=255)                                                                    tatGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCttt                                                         <  1:569550/140‑1 (MQ=255)                                                                     atGATCAAATAGCATTACCCCCGCCTCACAAAGTCGTGCATAGGGAACTCGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc                                                        <  2:447586/140‑1 (MQ=255)                                                                     atGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc                                                        <  1:964963/140‑1 (MQ=255)                                                                     atGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTGCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCTCCAATTTTCTGGAAATCCTATGATGCCTTTc                                                        <  1:74442/140‑1 (MQ=255)                                                                      tGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTcc                                                       <  2:653443/140‑1 (MQ=255)                                                                        aTCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg                                                     <  1:518967/140‑1 (MQ=255)                                                                        aTCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg                                                     <  1:512890/140‑1 (MQ=255)                                                                          cAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGt                                                   <  2:968489/140‑1 (MQ=255)                                                                            aaTAGCATTAACCCCGCCTCACGACCTCCTGCATAGGTAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaa                                                 <  1:281610/140‑1 (MQ=255)                                                                                   ttAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCATGCGCACCCACGACTAAATTTCCACCCCTTTAAACTTCTCAATAATCTCACCAATGTTCAGCAGATCCTATGCTTCCTTTCCCGGTAACTGgcgc                                          >  2:331534/1‑140 (MQ=255)                                                                                    tAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATg                                              >  2:865484/1‑135 (MQ=255)                                                                                    tAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATg                                              <  1:865484/135‑1 (MQ=255)                                                                                           cccTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGtt                                  >  2:933262/1‑140 (MQ=255)                                                                                           cccTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGtt                                  >  2:924609/1‑140 (MQ=255)                                                                                            ccTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTc                                 >  1:541128/1‑140 (MQ=255)                                                                                            ccTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTc                                 >  1:176311/1‑140 (MQ=255)                                                                                                   aCGTCCTGCATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGa                          <  1:243662/140‑1 (MQ=255)                                                                                                   aCGTCCTGCATAGGGAACACGTCTTCCCCTGCGCAACCATGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGa                          <  2:427562/140‑1 (MQ=255)                                                                                                        cTGCATAGGGAACCCGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTg                     <  1:107964/140‑1 (MQ=255)                                                                                                           cATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCaa                  >  1:563089/1‑140 (MQ=255)                                                                                                           cATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCaa                  >  1:676115/1‑140 (MQ=255)                                                                                                           cATAGGGAACACGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCaa                  >  1:120811/1‑140 (MQ=255)                                                                                                                   acacGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTg                     >  2:759338/1‑129 (MQ=255)                                                                                                                   acacGTTTTCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTg                     <  1:759338/129‑1 (MQ=255)                                                                                                                      cGTTTTCCCCTGCGCTCCCACGGCTTAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCAt       <  1:354306/140‑1 (MQ=255)                                                                                                                           tCCCCTGCGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGaaa  <  2:981815/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     TGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAA  >  NC_000913/2767353‑2767614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑