Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,834,011	G→A	A34T (GCC→ACC)	norW →	NADH:flavorubredoxin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,834,011	0	G	A	100.0%	133.5 / NA	47	A34T (GCC→ACC)	norW	NADH:flavorubredoxin reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/23);  total (24/23)

GCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCA  >  NC_000913/2833875‑2834142                                                                                                                                         |                                                                                                                                    gCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTaccg                                                                                                                                  >  2:539949/1‑140 (MQ=255)     aGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGa                                                                                                                              <  2:82859/140‑1 (MQ=255)          tCTTTGAAGAACTGGCAACGGATGCAAAATGTGTACCGGTATTGTGATTATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCa                                                                                                                         <  2:425612/140‑1 (MQ=255)            tttGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATAAACCCTGATTACCGCCGACAGCATg                                                                                                                       >  1:421203/1‑140 (MQ=255)                  gaaCTGGCATCGGAGGCGAAATAAGTAACGGTATTTTGATCACCGGTTTGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAGCCCTGATTACCGCCGACAGCATGGATGAg                                                                                                                 <  2:327495/140‑1 (MQ=255)                  gaaCTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAg                                                                                                                 <  1:539949/140‑1 (MQ=255)                        gCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAAGTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTacaac                                                                                                           >  1:917520/1‑140 (MQ=255)                          aTCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTAcaacaa                                                                                                         >  2:780091/1‑140 (MQ=255)                             ggAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCTTGATTACGGCCGACGGCATGGGTGAGTACAACAAAcc                                                                                                      >  1:327495/1‑140 (MQ=255)                              gAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCt                                                                                                     <  1:780091/140‑1 (MQ=255)                                 gCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGAc                                                                                                  <  1:888126/140‑1 (MQ=255)                                 gCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGAc                                                                                                  <  1:802178/140‑1 (MQ=255)                                  cAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGAcc                                                                                                 >  1:578256/1‑140 (MQ=255)                                           tAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCTACAGCATGGATGAGTACAACACACCTGACCTCAGCCATg                                                                                        >  2:331146/1‑140 (MQ=255)                                           tAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATg                                                                                        >  1:947471/1‑140 (MQ=255)                                               ggCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTAt                                                                                    <  1:801742/140‑1 (MQ=255)                                               ggCATTGTGATCATCCGTTCGGGCTGCGCCGCCCGCCTACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTAt                                                                                    <  1:449920/140‑1 (MQ=255)                                                   ttGTGATCATCGGTTTGGGCTTTGCCGCCCGCCAACTAGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTAtcagt                                                                                <  1:398356/140‑1 (MQ=255)                                                   ttGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTAtcagt                                                                                >  2:843576/1‑140 (MQ=255)                                                           atcGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCa                                                                        <  2:947471/140‑1 (MQ=255)                                                                ttCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGATCTCAGCCATGTTATCAGTCAGGGGCAACGTg                                                                   >  2:437272/1‑140 (MQ=255)                                                                  cGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGcc                                                                 >  1:39349/1‑140 (MQ=255)                                                                   gggCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCg                                                                <  1:74254/140‑1 (MQ=255)                                                                   gggCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCg                                                                <  1:437272/140‑1 (MQ=255)                                                                         cgccgcCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGAcc                                                          >  1:210764/1‑140 (MQ=255)                                                                          gccgccCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCt                                                         >  1:733198/1‑140 (MQ=255)                                                                              cccgccAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTAcc                                                     <  1:925426/140‑1 (MQ=255)                                                                               ccgccAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACTGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTAccc                                                    >  1:993320/1‑140 (MQ=255)                                                                               ccgccAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTAccc                                                    >  1:140752/1‑140 (MQ=255)                                                                                         gTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGAcggc                                          >  2:99172/1‑140 (MQ=255)                                                                                          tGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGAcggcg                                         <  2:39349/140‑1 (MQ=255)                                                                                                 tatTCGCAAACAGGACGCCACTATTCCATTACCCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAAt                                  <  2:15112/140‑1 (MQ=255)                                                                                                 tatTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAAt                                  >  2:773420/1‑140 (MQ=255)                                                                                                   ttCGCAAACAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAAttt                                >  1:923261/1‑140 (MQ=255)                                                                                                        aaaCAGGCGGCCACTTGACCATGACCCCTTAGTTCGGCCGACAGCTTGTATGAGTACAACAAACCTGGCCCCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGa                           <  2:475061/140‑1 (MQ=255)                                                                                                        aaaCAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGa                           <  2:140752/140‑1 (MQ=255)                                                                                                         aaCAGGACGCCACTATTCCATTCACTCTGCTTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAg                          <  1:331146/140‑1 (MQ=255)                                                                                                         aaCAGGACGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAg                          <  1:99172/140‑1 (MQ=255)                                                                                                              gACGCCACTATTCCATTACCCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGtt                     <  1:720313/140‑1 (MQ=255)                                                                                                               aCGCCACTATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGACAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGttt                    >  2:16038/1‑140 (MQ=255)                                                                                                                      tATTCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGCGAGTTTGCCTAGCAGTTTAATCTGc             >  1:480607/1‑140 (MQ=255)                                                                                                                        ttCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGAGCTTACCCGACAGGCGGTGGGTGAATTTGCCAAGCAGTTTAATCTGCAc           >  2:312335/1‑140 (MQ=255)                                                                                                                        ttCCATTAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCCGACGGCGGGTGTATTTGCCGATCAGTTTAATCTGCAc           >  1:393516/1‑140 (MQ=255)                                                                                                                            aTTAACCCTGATTACCGTCGACAGCATGGATAAGTACAAAAAACCTGAGCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGt       <  2:421203/140‑1 (MQ=255)                                                                                                                             ttAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGtt                     >  1:862394/1‑125 (MQ=255)                                                                                                                             ttAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGtt                     <  2:862394/125‑1 (MQ=255)                                                                                                                             ttAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGtt      >  1:578726/1‑140 (MQ=255)                                                                                                                             ttAACCCTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGtt      >  2:874372/1‑140 (MQ=255)                                                                                                                                 cccTGATTACCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCca  <  1:773420/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCA  >  NC_000913/2833875‑2834142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑