Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,845,002	G→A	N490N (AAC→AAT)	hycE ←	formate hydrogenlyase subunit HycE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,845,002	0	G	A	100.0%	168.9 / NA	50	N490N (AAC→AAT)	hycE	formate hydrogenlyase subunit HycE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/32);  total (18/32)

GGTACAGGAGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGGTTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGTCG  >  NC_000913/2844867‑2845137                                                                                                                                        |                                                                                                                                        ggtACAGGAGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCATCGCCAGCGGCACAGCTTCTGATTGt                                                                                                                                     >  1:429793/1‑140 (MQ=255) ggtACAGGAGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGt                                                                                                                                     >  1:1010230/1‑140 (MQ=255)    aCAGGAGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGc                                                                                                                                  <  1:143324/140‑1 (MQ=255)    aCAGGAGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGc                                                                                                                                  <  2:175243/140‑1 (MQ=255)       ggAGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCTgg                                                                                                                               <  2:710387/140‑1 (MQ=255)       ggAGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCgg                                                                                                                               <  2:963504/140‑1 (MQ=255)         aGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTc                                                                                                                             <  1:164552/140‑1 (MQ=255)         aGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTc                                                                                                                             >  2:84094/1‑140 (MQ=255)          gTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCa                                                                                                                            <  2:968235/140‑1 (MQ=255)          gTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCa                                                                                                                            <  2:916313/140‑1 (MQ=255)             gCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCATCGCCAGCTGTAAAGTTTCTGATTGTCGCCGGTCATGc                                                                                                                         >  1:370694/1‑140 (MQ=25)             gCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGc                                                                                                                                  <  2:916822/131‑1 (MQ=255)             gCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGc                                                                                                                                  >  1:916822/1‑131 (MQ=255)             gCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGc                                                                                                                         >  1:595364/1‑140 (MQ=255)             gCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGc                                                                                                                         >  1:41817/1‑140 (MQ=255)              cAAGGGTCGAGGCTACCGATAACCAGCGGCGCATCGGAAACGGTGTTGACGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCt                                                                                                                        <  2:313876/140‑1 (MQ=255)               aaGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTc                                                                                                                       <  2:595364/140‑1 (MQ=255)                 gggTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCa                                                                                                                     <  1:954195/140‑1 (MQ=255)                 gggTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCa                                                                                                                     <  2:930341/140‑1 (MQ=255)                  ggTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGc                                                                                                                                  <  1:609300/126‑1 (MQ=255)                  ggTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGc                                                                                                                                  >  2:609300/1‑126 (MQ=255)                  ggTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAg                                                                                                                    >  1:65878/1‑140 (MQ=255)                  ggTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGGGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAg                                                                                                                    >  1:706394/1‑140 (MQ=255)                                    tCAGCGGCGCATCGGAAACGGTGTTTCCGAGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCcgcgc                                                                                                  <  1:319875/140‑1 (MQ=255)                                         ggcgCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGgcgc                                                                                             >  2:782855/1‑140 (MQ=255)                                                            tGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCa                                                                          <  1:745140/140‑1 (MQ=255)                                                                     gcaTGTAGCGCAGGGTCGGCCATTTCGCGTATGTCGCGGCACGGCAGCACCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGc                                                                 <  2:370694/140‑1 (MQ=255)                                                                     gcaTGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGc                                                                 <  2:1010230/140‑1 (MQ=255)                                                                     gcaTGTAGCGCAGGGCCGGCCACTTCGCGCAGGTCGCTGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGc                                                                 <  1:261958/140‑1 (MQ=38)                                                                      caTGTAGCGCAGGGTCGGCCAGGTCTCGTCGGTCGCGTCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCACCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCg                                                                <  1:441673/140‑1 (MQ=255)                                                                           aGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGTAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATg                                                           <  2:959766/140‑1 (MQ=255)                                                                                aGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGt                                                      >  2:82745/1‑140 (MQ=255)                                                                                aGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGt                                                      >  1:80251/1‑140 (MQ=255)                                                                                  ggTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTa                                                     >  1:503787/1‑139 (MQ=255)                                                                                  ggTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTa                                                     <  2:503787/139‑1 (MQ=255)                                                                                           gTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTcca                                           <  2:79381/140‑1 (MQ=255)                                                                                            ttCGCGTAGTTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTccac                                          >  1:954083/1‑140 (MQ=255)                                                                                            ttCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTccac                                          >  2:952611/1‑140 (MQ=255)                                                                                             tCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTccacc                                         <  2:823661/140‑1 (MQ=255)                                                                                             tCGCGAAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTccacc                                         <  1:858663/140‑1 (MQ=255)                                                                                                cgTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATc                                      <  1:82745/140‑1 (MQ=255)                                                                                                                 caGCGCCAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAg                     >  2:871491/1‑140 (MQ=255)                                                                                                                      ccAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGTAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTAt                >  1:211396/1‑140 (MQ=255)                                                                                                                      ccAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTACACCATAAGTGGGTCACCCGGCAGGTTAt                >  1:436189/1‑140 (MQ=255)                                                                                                                       cAGCGGTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTATc               <  1:818059/140‑1 (MQ=255)                                                                                                                           ggTACAGCTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGa           <  1:934202/140‑1 (MQ=255)                                                                                                                                  cTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGt    <  2:84828/140‑1 (MQ=255)                                                                                                                                  cTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGt    <  1:84094/140‑1 (MQ=255)                                                                                                                                  cTTCTGATTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGt    <  2:954083/140‑1 (MQ=255)                                                                                                                                  cTTCTGATTGTCGCCGGTCATGCTCCAGTAGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGt    <  1:669294/140‑1 (MQ=255)                                                                                                                                    tCTGATTGTCGCCGGTCATGCTTCAGTGGATTTCATCGCCGCGCGGCGGTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGTCg  <  2:430585/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GGTACAGGAGTAGCAAGGGTCGAGGCTACCGATAATCAGCGGCGCATCGGAAACGGTGTTGCCGCGCAGCATGTAGCGCAGGGTCGGCCAGTTCGCGTAGGTCGCGGCACGGCAGCGCCAGCGGTACAGCTTCTGGTTGTCGCCGGTCATGCTCCAGTGGATATCATCGCCGCGCGGCGCTTCGGCAAAGCCCAGCGCAAAGCGGTGCGGAATGTAGGTAAAGCCTTCCACCATCAGTGGGCCACCCGGCAGGTTATCCAGACCGTAGTCG  >  NC_000913/2844867‑2845137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑