Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,886,819	T→C	D134G (GAT→GGT)	cas3 ←	CRISPR‑associated endonuclease/helicase Cas3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,886,819	0	T	C	100.0%	300.6 / NA	82	D134G (GAT→GGT)	cas3	CRISPR‑associated endonuclease/helicase Cas3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/46);  total (36/46)

GAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCC  >  NC_000913/2886682‑2886948                                                                                                                                          |                                                                                                                                  gagaAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCCTAAGGATGAGGAGCGGGATCACAAAAACTGAAAAAAccc                                                                                                                                 >  2:326561/1‑140 (MQ=255)    aaGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGACCGGAATCAAAAAAACTGAAAAAACCCCCg                                                                                                                              >  1:389836/1‑140 (MQ=255)         ggCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCgagaga                                                                                                                         <  1:107317/140‑1 (MQ=255)          gCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGAt                                                                                                                        <  1:152441/140‑1 (MQ=255)          gCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGAt                                                                                                                        >  2:537637/1‑140 (MQ=255)                cccAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGGAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGctct                                                                                                                  >  1:182823/1‑140 (MQ=255)                 ccAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTg                                                                                                                 <  2:561677/140‑1 (MQ=255)                         aCTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGACAAAACCCCAGAGACATTGCTCTGAAAGTGaa                                                                                                         >  2:421072/1‑140 (MQ=255)                         aCTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGaa                                                                                                         >  1:1002117/1‑140 (MQ=255)                          cTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAAt                                                                                                        >  2:754238/1‑140 (MQ=255)                           ttATCTTGATCCTGGGAATGTAATATAAAACCATGCTGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATc                                                                                                       <  2:425186/140‑1 (MQ=255)                           ttATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATTGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATc                                                                                                       <  2:645709/140‑1 (MQ=255)                           ttATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGGAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATc                                                                                                       <  2:182823/140‑1 (MQ=255)                           ttATCTTGATCCTGGCAATGTATTACAAACCCATGATTTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATc                                                                                                       <  1:261371/140‑1 (MQ=255)                                ttGATCCTGGGAATGTAATATAGAACCATGACGTCCTGTCACGGCCTCCACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                                                  <  2:389836/140‑1 (MQ=255)                                ttGATCCTGGGAATGTAATATACAATCATAATGTCCTTTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                                                  <  1:421072/140‑1 (MQ=255)                                ttGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                                                  <  1:361494/140‑1 (MQ=255)                                ttGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                                                  <  2:631526/140‑1 (MQ=255)                                ttGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                                                  <  2:148112/140‑1 (MQ=255)                                ttGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                                                  <  2:761072/140‑1 (MQ=255)                                ttGATCCTGGGAATGTAATATAAAACCATGATGTCCTCTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                                                  <  1:405274/140‑1 (MQ=255)                                gagacagtGGGAATGTAATATACAACCATCATGTCCTATAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGt                                                                                                  <  1:268595/133‑1 (MQ=255)                                    tCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTaaa                                                                                              >  1:802128/1‑140 (MQ=255)                                     ccTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAAc                                                                                             <  2:765126/140‑1 (MQ=255)                                     ccTGGGAATGTAATACAAAACCCTGGTGTACTGTAACTGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAAc                                                                                             <  1:279586/140‑1 (MQ=255)                                      cTGGGAATGTAATATAAAACCACGAAGTCCTGTAACGACCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAAcc                                                                                            <  1:264431/140‑1 (MQ=255)                                        gggAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCTGAGAGATAGCTTTGGAAGTGATTCCTGGTTAAACCaa                                                                                          >  1:376234/1‑140 (MQ=255)                                         ggAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCGCGAAAGATTGCTCTGAGAGTGAATCCTGGTTAAACCAAt                                                                                         >  2:340512/1‑140 (MQ=255)                                         ggAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAAt                                                                                         >  2:129013/1‑140 (MQ=255)                                            aTGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACa                                                                                      >  2:820258/1‑140 (MQ=255)                                             tGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAg                                                                                     >  2:637468/1‑140 (MQ=255)                                              gTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGa                                                                                    >  2:951878/1‑140 (MQ=255)                                                  tataAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCTCGAGAGATTGCTGTGAGAGTGAATCGTGGTGAAACCAATAcctgtctc                                                                                >  2:268595/1‑133 (MQ=255)                                                       aaCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCa                                                                           >  2:830467/1‑140 (MQ=255)                                                        aCCATGATGTCCTGTCACGGCCCCTTCCCCTGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCAc                                                                          <  1:314569/140‑1 (MQ=255)                                                        aCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCAc                                                                          >  2:538389/1‑140 (MQ=255)                                                        aCCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCACGACTCATAGGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCAc                                                                          <  2:434372/140‑1 (MQ=255)                                                         ccATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCAcc                                                                         >  2:760839/1‑140 (MQ=255)                                                         ccATGATGTCCTGTAACGGCCTATACCCATGGAAACCAGCACTCATAACGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCAcc                                                                         <  2:424846/140‑1 (MQ=255)                                                            tgatgTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATg                                                                      >  1:919463/1‑140 (MQ=255)                                                            tgatgTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATg                                                                      >  1:548004/1‑140 (MQ=255)                                                                    tGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCCATATAGACCGGCTGCACCATGATTAAAtt                                                              >  1:424846/1‑140 (MQ=255)                                                                    tGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTGAAAACAATACAGGCCGGTTGCACCATGATTAAAtt                                                              >  2:420948/1‑140 (MQ=255)                                                                      tAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTa                                                            <  1:760839/140‑1 (MQ=255)                                                                        aCGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACg                                                          <  1:830467/140‑1 (MQ=255)                                                                         cGGCCTCTACCCATGGAAACCATGATTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                                                         <  1:420948/140‑1 (MQ=255)                                                                         cGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                                                         <  1:969820/140‑1 (MQ=255)                                                                         cGGCCCTTACCCTTGGACACCAGAACTCATCAGGATGATGAGCGCTATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACgg                                                         <  1:635427/140‑1 (MQ=255)                                                                           gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTa                                                            >  2:969677/1‑135 (MQ=255)                                                                           gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTa                                                            <  1:969677/135‑1 (MQ=255)                                                                           gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACggaa                                                       >  1:793365/1‑138 (MQ=255)                                                                           gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGca                                                       <  1:61794/140‑1 (MQ=255)                                                                           gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGca                                                       >  1:175748/1‑140 (MQ=255)                                                                           gCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGca                                                       <  2:919463/140‑1 (MQ=255)                                                                             ctctACCCATGGAAAACAGGACTCACAAGAATGAGGATCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGcaca                                                     <  1:326561/140‑1 (MQ=255)                                                                              tctACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACAt                                                    <  1:129013/140‑1 (MQ=255)                                                                                tACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACAttt                                                  >  2:637335/1‑140 (MQ=255)                                                                                 aCCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTtg                                                 <  1:148721/140‑1 (MQ=255)                                                                                      tGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTg                                            >  1:592975/1‑140 (MQ=255)                                                                                        gAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGAt                                          <  2:548004/140‑1 (MQ=255)                                                                                        gAAACCAGGACTCATAAAGATGTGGAGCGGCGTCACAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGAt                                          <  2:376234/140‑1 (MQ=255)                                                                                          aaCCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATgg                                        <  1:820258/140‑1 (MQ=255)                                                                                           aCCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGa                                       <  1:979909/140‑1 (MQ=255)                                                                                           aCCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGa                                       <  2:772228/140‑1 (MQ=255)                                                                                           aCCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGAACATTTGTGTTGATGGa                                       <  2:793365/140‑1 (MQ=255)                                                                                            ccAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGAc                                      >  2:913082/1‑140 (MQ=255)                                                                                                gACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCAtt                                  >  2:245146/1‑140 (MQ=255)                                                                                                     aTAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTg                             >  2:912843/1‑140 (MQ=255)                                                                                                     aTAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTg                             >  2:620657/1‑140 (MQ=255)                                                                                                          gATGAGGAGCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGc                        >  2:101571/1‑140 (MQ=255)                                                                                                             gaggagCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACAGCACATTGGTGATGGTGGACCGTTAAGTGATGGCGtt                     >  1:300591/1‑140 (MQ=255)                                                                                                              aggagCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTg                    <  1:620657/140‑1 (MQ=255)                                                                                                              aggagCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTg                    <  1:538389/140‑1 (MQ=255)                                                                                                              aggagCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTg                    <  1:754238/140‑1 (MQ=255)                                                                                                              aggagCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGACTAAATTTAAGGCACATATGTGTTGAAGGACTATTAAGTGATGGCGTTg                    >  2:373377/1‑140 (MQ=255)                                                                                                               ggcgCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGc                   <  1:951878/137‑1 (MQ=255)                                                                                                                gagCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTg                    <  2:763327/138‑1 (MQ=255)                                                                                                                gagCGGCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTg                    >  1:763327/1‑138 (MQ=255)                                                                                                                    ggCATCAAAAAAACTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGAt              >  1:55592/1‑140 (MQ=255)                                                                                                                          aaaaaaaCTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAAtt        >  2:563621/1‑140 (MQ=255)                                                                                                                               aaCTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGc   <  2:802128/140‑1 (MQ=255)                                                                                                                               aaCTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGc   <  2:175748/140‑1 (MQ=255)                                                                                                                                aCTGAAAAAACCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGcc  <  1:912843/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  GAGAAGCTGGCATTTCCCAACGCGACTTATCTTGATCCTGGGAATGTAATATAAAACCATGATGTCCTGTAACGGCCTCTACCCATGGAAACCAGGACTCATAAGGATGAGGAGCGGCATCAAAAAAACTGAAAAAATCCCCGAGAGATTGCTCTGAAAGTGAATCCTGGTTAAACCAATACAGACCGGCTGCACCATGATTAAATTTACGGCACATTTGTGTTGATGGACCATTAAGTGATGGCGTTGCAGGATTTAATTTCAGCC  >  NC_000913/2886682‑2886948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑