Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,415	A→G	V237A (GTG→GCG)	kduI ←	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,415	0	A	G	100.0%	293.5 / NA	85	V237A (GTG→GCG)	kduI	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/44);  total (41/44)

CCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATGG  >  NC_000913/2983282‑2983550                                                                                                                                      |                                                                                                                                        ccACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCt                                                                                                                                   <  2:571634/140‑1 (MQ=255)  cacaACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTg                                                                                                                                  <  1:791000/140‑1 (MQ=255)   acaACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGc                                                                                                                                 >  1:850538/1‑140 (MQ=255)    caACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCt                                                                                                                                <  1:786903/140‑1 (MQ=255)     aaCTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTc                                                                                                                               >  2:562213/1‑140 (MQ=255)     aaCTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTc                                                                                                                               >  1:754521/1‑140 (MQ=255)      aCTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCg                                                                                                                              <  1:554338/140‑1 (MQ=255)        tAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGtt                                                                                                                            <  2:700482/140‑1 (MQ=255)          gcgcAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTAt                                                                                                                          >  2:3740/1‑140 (MQ=255)           cgcAAATCTTTAACGGCCACATGGTCCATATGATCGAAGCCCTGGTTTCCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATg                                                                                                                         <  2:370867/140‑1 (MQ=255)           cgcAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGAAACCGGAATAGATCGACGAGCTCGGGAAGATAGCCGCCTGCTCGTTATg                                                                                                                         >  2:290300/1‑140 (MQ=255)           agacagtCTTTAACGTCCACATCGTGCATTTCATCAAGGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATg                                                                                                                         <  1:482557/134‑1 (MQ=255)               aaTCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATc                                                                                                                     >  2:737253/1‑140 (MQ=255)               aaTCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATc                                                                                                                     <  2:754521/140‑1 (MQ=255)               aaTCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCAGAATGGATGGACCAGCAGGGGGAGATCGCCACCTGCTCGGTATGCATc                                                                                                                     >  2:495830/1‑140 (MQ=255)                            cacaTGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGAACGCCGCCTGCTCCTTATAAATCACAatatctgtct                                                                                                        >  2:482557/1‑134 (MQ=255)                               aTGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGttt                                                                                                     >  1:106723/1‑140 (MQ=255)                               aTGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGttt                                                                                                     >  2:714875/1‑140 (MQ=255)                                     cATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCTTGCTCGTAATGCATTACAATAGGACGCGTTTCTTGCg                                                                                               >  1:330447/1‑140 (MQ=255)                                     cATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCg                                                                                               >  1:522082/1‑140 (MQ=255)                                      atatCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCgg                                                                                              <  1:176022/140‑1 (MQ=255)                                        atcatcAAAGACCTGGTTTTCAGCGACCATGACCCGGATGAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCt                                                                                            <  2:330447/140‑1 (MQ=255)                                         tcatcaAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTg                                                                                           <  1:562213/140‑1 (MQ=255)                                          catcaAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGc                                                                                          <  1:878679/140‑1 (MQ=255)                                           atcaAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGcc                                                                                         >  1:661693/1‑140 (MQ=255)                                            tcaAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGccc                                                                                        >  1:951876/1‑140 (MQ=255)                                            tcaAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGccc                                                                                        >  2:992577/1‑140 (MQ=255)                                             caAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCgg                                                                                              <  1:239200/133‑1 (MQ=255)                                             caAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCgg                                                                                              >  2:239200/1‑133 (MQ=255)                                             caAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGcccc                                                                                       >  2:43250/1‑140 (MQ=255)                                             caAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGcccc                                                                                       >  2:454163/1‑140 (MQ=255)                                             caAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGcccc                                                                                       >  2:699768/1‑140 (MQ=255)                                                aGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCcatc                                                                                    <  2:661693/140‑1 (MQ=255)                                                   ccTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCAACACAATAAGACCCGCTTCTTGCGGCTGCCCCATCAtg                                                                                 >  1:406263/1‑140 (MQ=255)                                                   ccTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAACCTTTGGTTCCGACAGCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATCTGACGCGTTTCTCGCGGCTGCCCCATCAtg                                                                                 >  1:863378/1‑140 (MQ=255)                                                    cTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCAtgt                                                                                >  2:86311/1‑140 (MQ=255)                                                    cTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCAtgt                                                                                >  2:71550/1‑140 (MQ=255)                                                            cACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg                                                                        <  1:3740/140‑1 (MQ=255)                                                            cACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACg                                                                        >  1:1856/1‑140 (MQ=255)                                                              ccGACCATGCCCCAGATAAAGGTATAAGCCTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCA‑AATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAg                                                                     <  1:360769/140‑1 (MQ=255)                                                               cGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAg                                                                     <  1:71550/140‑1 (MQ=255)                                                                gACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg                                                                    <  1:56267/140‑1 (MQ=255)                                                                gACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg                                                                    <  1:248718/140‑1 (MQ=255)                                                                gACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg                                                                    <  2:951876/140‑1 (MQ=255)                                                                 aCCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg                                                                    >  1:1011431/1‑139 (MQ=255)                                                                 aCCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAgg                                                                    <  2:1011431/139‑1 (MQ=255)                                                                 aCCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGc                                                                   >  2:17683/1‑140 (MQ=255)                                                                    aTGCCCCGGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGtc                                                                <  1:204504/140‑1 (MQ=255)                                                                    aTGCCCCAGATAAAGGTATAAGGTTTGGTTCCGACTCCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGtc                                                                <  2:360083/140‑1 (MQ=255)                                                                    aTGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGtc                                                                <  2:851066/140‑1 (MQ=255)                                                                     tGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAg                                                                     <  2:724651/134‑1 (MQ=255)                                                                     tGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAg                                                                     >  1:724651/1‑134 (MQ=255)                                                                         ccAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTcatcat                                                           <  1:454163/140‑1 (MQ=255)                                                                          cAGATAAAGGTATAACCTTTGGATCAGACCCCGGAATCGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCatcatc                                                          <  1:282203/140‑1 (MQ=255)                                                                            gATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCa                                                        <  1:589637/140‑1 (MQ=255)                                                                            gATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCa                                                        >  1:940092/1‑140 (MQ=255)                                                                              tAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCata                                                      <  2:106723/140‑1 (MQ=255)                                                                                  ggTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATATGGAAAACACAGGTGTCATCATCCATATTga                                                  >  2:375817/1‑140 (MQ=255)                                                                                   gTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaa                                                 <  2:1856/140‑1 (MQ=255)                                                                                    tataAGCTTTTGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaa                                                <  1:521236/140‑1 (MQ=255)                                                                                    tataAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTgaaa                                                <  1:940365/140‑1 (MQ=255)                                                                                      taAGCTTTGGTTCCGACACCGGAATGGATCTACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGTCGTCATCATCCATATTgaaata                                              >  1:495255/1‑140 (MQ=255)                                                                                             tGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGACAACGCAGTCGTAATCATCCATCTTGAAATAGAAATaa                                       >  1:361107/1‑140 (MQ=255)                                                                                              ggTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATaaa                                      >  1:201911/1‑140 (MQ=255)                                                                                              ggTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATaaa                                      <  1:699768/140‑1 (MQ=255)                                                                                              ggTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATaaa                                      <  1:86311/140‑1 (MQ=255)                                                                                                      cacCGGAATGGATCGACGAGCTCGGGGAGATTGCCCCCTCCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATc                              <  2:406263/140‑1 (MQ=255)                                                                                                         cGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAGACGCAGGCCTCATCATCCATATTGACATAGGAATAAACTTCCATCCgg                           >  2:306614/1‑140 (MQ=255)                                                                                                         cGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAGACGCAGGCATCAACATGCATATTGATATAGGAATAAACTTCCATCCgg                           >  2:272452/1‑140 (MQ=255)                                                                                                         cGGAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCgg                           <  1:714875/140‑1 (MQ=255)                                                                                                           gAATGGATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGgcg                         >  2:59156/1‑140 (MQ=255)                                                                                                                gATCGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCGATATTGAGATAGAAGTAAACTGCCATCCGGCGCTCGt                    >  1:291035/1‑140 (MQ=255)                                                                                                                   cGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTggg                 >  2:693430/1‑140 (MQ=255)                                                                                                                   cGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTggg                 >  2:770144/1‑140 (MQ=255)                                                                                                                   cGACCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTggg                 >  2:217568/1‑140 (MQ=255)                                                                                                                     aCCAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGgtg               >  2:727254/1‑140 (MQ=255)                                                                                                                      ccAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGgtgt              >  2:22874/1‑140 (MQ=255)                                                                                                                       cAGCTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGtgtg             <  1:737253/140‑1 (MQ=255)                                                                                                                         gCTCGGGGAGATCGCCGACTGCTCGTTATGCCTCAGAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGac           <  2:347911/140‑1 (MQ=255)                                                                                                                          cTCGGGGAGATCGCCGCCTGCTGGTTATGAATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGaca          <  1:375817/140‑1 (MQ=255)                                                                                                                          cTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGaca          <  1:236130/140‑1 (MQ=255)                                                                                                                          cTCGGGGAGATCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATACATATTGAAATACAAAGAAACTTCCATCCGGCGCTCGTGGGTGTGaca          >  2:419907/1‑140 (MQ=255)                                                                                                                            cGGGGAGATCGCCGCCTGCTCGTTAGGCACCACAATACGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACg        <  1:290300/140‑1 (MQ=255)                                                                                                                                 agaTCGCCGCCTGCTGGTTATGTATCACAATATGACGAGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATg   <  1:377885/140‑1 (MQ=255)                                                                                                                                  gaTCGCCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATgg  <  2:940092/140‑1 (MQ=255)                                                                                                                                  gaTCGCCGCCTGCTCATTATGCATCACAAAATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATgg  <  1:495830/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCTGCCCCATCATGTGGAAAACGCAGGCGTCATCATCCATATTGAAATAGAAATAAACTTCCATCCGGCGCTCGTGGGTGTGACACGGCATGG  >  NC_000913/2983282‑2983550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑