Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,000,395	C→T	D17D (GAC→GAT)	xdhA →	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,000,395	0	C	T	100.0%	181.6 / NA	54	D17D (GAC→GAT)	xdhA	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/37);  total (17/37)

ACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGC  >  NC_000913/3000258‑3000531                                                                                                                                          |                                                                                                                                         aCTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACtgatga                                                                                                                                        <  1:131200/140‑1 (MQ=255) aCTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACtgatga                                                                                                                                        <  2:188253/140‑1 (MQ=255) aCTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACtgatga                                                                                                                                        >  1:960872/1‑140 (MQ=255)      aTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAGTCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGAttatg                                                                                                                                   >  2:142190/1‑140 (MQ=255)           tGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGAttatgttatg                                                                                                                              >  1:783213/1‑140 (MQ=255)            gCCATAAGAACCAAATGTTCAAATCGAGGGGGTCTCTATGGAAGCGCGCGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATgg                                                                                                                             <  2:447220/140‑1 (MQ=255)            gCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATgg                                                                                                                             <  1:103586/140‑1 (MQ=255)            gCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATgg                                                                                                                             <  2:953957/140‑1 (MQ=255)            gCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATgg                                                                                                                             <  2:945817/140‑1 (MQ=255)            gCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATgg                                                                                                                             <  2:877524/140‑1 (MQ=255)            gCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATgg                                                                                                                             <  1:710095/140‑1 (MQ=255)              cATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCg                                                                                                                           <  2:997245/140‑1 (MQ=255)                tAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCggg                                                                                                                         >  1:524945/1‑140 (MQ=255)                 aaGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGc                                                                                                                        <  2:960872/140‑1 (MQ=255)                 aaGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACAGGGCGAGGACGATATACTGATGATTATGTTATGGCgggg                                                                                                                        >  2:458442/1‑139 (MQ=255)                     aCCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATgg                                                                                                                             >  1:126291/1‑131 (MQ=255)                     aCCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATgg                                                                                                                             <  2:126291/131‑1 (MQ=255)                      ccAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCAtgtg                                                                                                                   <  1:120263/140‑1 (MQ=255)                      ccAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCAtgtg                                                                                                                   >  2:61622/1‑140 (MQ=255)                       cAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATgtgt                                                                                                                  <  2:155712/140‑1 (MQ=255)                         aaTGTTCAAATCGAGGGGATTTTTACGGAAGCGCGGGGAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTa                                                                                                                <  1:417058/140‑1 (MQ=255)                          aTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCTTGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTAt                                                                                                               >  2:746673/1‑140 (MQ=255)                           tGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATg                                                                                                              <  1:993188/140‑1 (MQ=255)                           tGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATg                                                                                                              <  1:970100/140‑1 (MQ=255)                             ttCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCg                                                                                                            <  1:164823/140‑1 (MQ=255)                              tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATAGACTGATGGTTAGGTGATGGCGGGGATGGGTTTTgggg                                                                                                           >  1:281154/1‑137 (MQ=255)                                      aGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGGATGTGTTTTGCGgaaaaatta                                                                                                   >  2:987543/1‑134 (MQ=255)                                       ggggATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTAc                                                                                                  >  2:637892/1‑140 (MQ=255)                                            tttCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGTCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATTGGAAAAATGTACGTAGc                                                                                             >  1:541711/1‑140 (MQ=255)                                            tttCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTTTGCGAAAAATTTAAGTAGc                                                                                             >  1:567990/1‑140 (MQ=255)                                                tATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTa                                                                                         <  1:81851/140‑1 (MQ=255)                                                tATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTa                                                                                         <  1:746673/140‑1 (MQ=255)                                                tATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTa                                                                                         <  1:687638/140‑1 (MQ=255)                                                tATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTa                                                                                         <  1:173319/140‑1 (MQ=255)                                                          cgGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAAAATTTTAGTAACCCCCTCGCACATgg                                                                               >  1:793346/1‑140 (MQ=255)                                                          cgGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCAAGTGGTTTTCGGAAGAAGTACGGAACCCTCCCCCACATgg                                                                               >  2:734380/1‑140 (MQ=255)                                                           gggAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGt                                                                              <  1:987543/140‑1 (MQ=255)                                                               aGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATg                                                                          <  1:734380/140‑1 (MQ=255)                                                                  aaCCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCg                                                                       <  1:100673/140‑1 (MQ=255)                                                                   aCCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGt                                                                      <  1:693955/140‑1 (MQ=255)                                                                   aCCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGt                                                                      <  1:38949/140‑1 (MQ=255)                                                                    ccGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTa                                                                     <  1:919575/140‑1 (MQ=255)                                                                    ccGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTa                                                                     <  2:502136/140‑1 (MQ=255)                                                                    ccGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTa                                                                     <  1:866269/140‑1 (MQ=255)                                                                    ccGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTa                                                                     <  2:114624/140‑1 (MQ=255)                                                                    ccGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTa                                                                     <  2:850212/140‑1 (MQ=255)                                                                         aCGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAGGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAt                                                                <  1:430226/140‑1 (MQ=255)                                                                         aCGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGGTATGCGAAAAATGTACGTAGCCCCATCGCCCCTGGGTATGCCGGAAGTAt                                                                >  2:48921/1‑140 (MQ=255)                                                                              tGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatg                                                           <  1:637892/140‑1 (MQ=255)                                                                              tGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatg                                                           <  1:48921/140‑1 (MQ=255)                                                                                   catgcatgCGCGTCGATCCCATCTCTAAGGTCACCCGGCCGGCACGATCTACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGaa                                                      <  2:281154/140‑1 (MQ=255)                                                                                                             aaGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCATGGGGTTTGCGAAAAATGTATGTAACCCTATCCCACCCGGGTATGGCGTAAGAATTTATTATTAAAAAAACAGAAGATTGCCGGGGGGACt                            >  2:157758/1‑140 (MQ=255)                                                                                                             aaGGTCACCGGGCGGGCACGATATACTGATGATTATGTTATGGCGGGCAAGGGGTATGAGAAATATTGACGTAACCCTATCGCGCCTGGGTTTTACGCAAATATTAATGATGAAAAAAACCGAAACTTGCCGGGCGGACt                            >  2:572988/1‑140 (MQ=255)                                                                                                                             cACGATATACTGATGTTTATGTCATCGCGGGCATGTGGTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTgg            <  1:412629/140‑1 (MQ=255)                                                                                                                                 atatACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACATGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAg        <  2:567990/140‑1 (MQ=255)                                                                                                                                 atatACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAg        <  2:710939/140‑1 (MQ=255)                                                                                                                                 atatACTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAg        <  2:541711/140‑1 (MQ=255)                                                                                                                                     aCTGATGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGAtgt    <  2:185127/140‑1 (MQ=255)                                                                                                                                       tgatgaTTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGc  <  1:572988/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAAGTTTGCCGGGCGTACTGGCGATTTTTACCTGGGAAGATGTGC  >  NC_000913/3000258‑3000531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑