Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 298,172 A→G R185R (CGT→CGC)  paoD ← molybdenum cofactor insertion chaperone for PaoABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913298,1720AG97.4% 123.2 / NA 38R185R (CGT→CGC) paoDmolybdenum cofactor insertion chaperone for PaoABC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (24/13);  minor base T (1/0);  total (25/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATCATGGCACAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGCGCAGCCCCACCGGTT  >  NC_000913/298038‑298305
                                                                                                                                      |                                                                                                                                     
agaTCATGGCACAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGGcc                                                                                                                                  >  2:900394/1‑140 (MQ=255)
  ttCATGGCACAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGt                                                                                                                                >  1:105511/2‑140 (MQ=255)
  aTCATGGCACAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGt                                                                                                                                >  2:1001242/1‑140 (MQ=255)
        gCACAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAatca                                                                                                                          <  1:882266/140‑1 (MQ=255)
              cAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGcc                                                                                                                    >  2:981335/1‑140 (MQ=255)
              cAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGcc                                                                                                                    >  1:670544/1‑140 (MQ=255)
                 aaTGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGa                                                                                                                 >  1:531123/1‑140 (MQ=255)
                  aTGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGAc                                                                                                                >  1:599322/1‑140 (MQ=255)
                    gACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGc                                                                                                              <  2:670544/140‑1 (MQ=255)
                       cgccgTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATg                                                                                                           >  1:683716/1‑140 (MQ=255)
                             atcggtatcgATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATTGCCTGa                                                                                                     >  2:888466/1‑140 (MQ=255)
                                 gtatcgATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAAccc                                                                                                 <  2:683716/140‑1 (MQ=255)
                                           gAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGATCGTCCGTAGATCATAAGTCTGACGCCTGGCGTGAACCCCCCCTCAAAg                                                                                       >  2:471684/1‑140 (MQ=255)
                                                 tggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCa                                                                                          <  1:501097/131‑1 (MQ=255)
                                                 tggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCa                                                                                          >  2:501097/1‑131 (MQ=255)
                                                 tggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTg                                                                                 >  2:796259/1‑140 (MQ=255)
                                                 tggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTg                                                                                 >  2:843003/1‑140 (MQ=255)
                                                         gCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCCCAAAGCCATTGAGATTCCa                                                                         <  2:658780/140‑1 (MQ=255)
                                                             gAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGACCTGAACCCCATCTCAAAGCCATTGAGATTCCAGccc                                                                     >  1:425341/1‑140 (MQ=255)
                                                               aaaaGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGt                                                                   <  1:843003/140‑1 (MQ=255)
                                                                aaaGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTc                                                                  <  1:981335/140‑1 (MQ=255)
                                                                             atGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTgg                                                     >  1:126233/1‑140 (MQ=255)
                                                                             atGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTgg                                                     <  2:866894/140‑1 (MQ=255)
                                                                             atGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTgg                                                     >  2:8786/1‑140 (MQ=255)
                                                                              tGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTggg                                                    >  1:1013111/1‑140 (MQ=255)
                                                                                     cATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGATTGCGTGGGCAGGcac                                             >  1:573592/1‑140 (MQ=255)
                                                                                              tgGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCCTTGAGATTGCAGACCGCCCGGGTTTGCGTGGGCAGGCACACCAGCGAt                                    >  2:383797/1‑140 (MQ=255)
                                                                                              tgGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGAt                                    >  2:630130/1‑140 (MQ=255)
                                                                                              tgGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGATTACGTGGGGAGGCACACCAGCGAt                                    >  1:420014/1‑140 (MQ=255)
                                                                                               ggCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGAtt                                   >  1:116743/1‑140 (MQ=255)
                                                                                                        aaGACTCGCGGTTGCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCg                          >  1:112431/1‑140 (MQ=255)
                                                                                                                     gCCTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGCGCAg             <  2:112431/140‑1 (MQ=255)
                                                                                                                      ccTGCGCCTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGCGCAGc            <  2:1013111/140‑1 (MQ=255)
                                                                                                                             cTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGCGCAGCCCCACCg     >  1:881169/1‑140 (MQ=255)
                                                                                                                             cTCAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGATTGTGCGTGCGGATCGTAGCGCAGCCCCACCg     >  1:381896/1‑140 (MQ=255)
                                                                                                                               cAAGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGCGCAGCCCCACCGGt   <  2:116743/140‑1 (MQ=255)
                                                                                                                                aaGAGAGCGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGATTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGCGCAGCCCCACCGGtt  <  2:573592/140‑1 (MQ=255)
                                                                                                                                aaGAGAGCGTCCGTAAACCATCAGCCTGACGTATGGCCTTAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGCGCAGCCCCACCGGtt  <  2:399080/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
AGATCATGGCACAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCAGCCCGTCCGGGTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGCGCAGCCCCACCGGTT  >  NC_000913/298038‑298305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑