Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,002,278	T→C	V645A (GTA→GCA)	xdhA →	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,002,278	0	T	C	100.0%	220.9 / NA	61	V645A (GTA→GCA)	xdhA	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/23);  total (38/23)

AACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGATCTGCCACAACT  >  NC_000913/3002145‑3002410                                                                                                                                      |                                                                                                                                     aaCCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACAcgg                                                                                                                                <  1:176580/140‑1 (MQ=255)   cccACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACAcggcg                                                                                                                              >  1:571342/1‑140 (MQ=255)     cACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCgga                                                                                                                            <  1:89469/140‑1 (MQ=255)     cACCGGCGTTTGGCTATACCTTAGTCGATGTGACGGTAGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCgga                                                                                                                            <  1:266444/140‑1 (MQ=255)      aCCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCggaa                                                                                                                           <  2:120322/140‑1 (MQ=255)       ccGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACAcgg                                                                                                                                >  1:686777/1‑134 (MQ=255)       ccGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACAcgg                                                                                                                                <  2:686777/134‑1 (MQ=255)       ccGGCGTTTGGCTGTACCTTTGTTGATATGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCggaat                                                                                                                          >  1:245559/1‑140 (MQ=255)        cGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAGTCCACTGCTGGTAGAAGGTCAGGCACACGGCggaatg                                                                                                                         >  1:379477/1‑140 (MQ=255)                        cTTTGTTGATCTGACGGTCTATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCt                                                                                                         <  1:501961/140‑1 (MQ=255)                          ttgttgATCTGACGGTCGATATTGCGCTGTACAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTgg                                                                                                       >  1:248045/1‑140 (MQ=255)                           tgttgATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTggg                                                                                                      >  2:659004/1‑140 (MQ=255)                            gttgATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGgc                                                                                                     <  1:838887/140‑1 (MQ=255)                             ttgATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGgcg                                                                                                    >  2:737614/1‑140 (MQ=255)                                 tCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTAt                                                                                                <  1:566580/140‑1 (MQ=255)                                   tGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTAttt                                                                                              <  2:748555/140‑1 (MQ=255)                                      cGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGaa                                                                                           <  2:772604/140‑1 (MQ=255)                                      cGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGaa                                                                                           <  1:799399/140‑1 (MQ=255)                                       ggTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAag                                                                                          >  1:130663/1‑140 (MQ=255)                                          cGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAgaga                                                                                       >  2:613532/1‑140 (MQ=255)                                              atTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAgatgat                                                                                   <  2:571342/140‑1 (MQ=255)                                                  cgcTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGatcatc                                                                               >  2:889425/1‑140 (MQ=255)                                                  cgcTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGatcatc                                                                               >  1:850534/1‑140 (MQ=255)                                                   gcTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCGTGGGCTGGGCGCTATTAGAAGAGATGATCATCg                                                                              >  2:458801/1‑140 (MQ=255)                                                       tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGc                                                                          >  2:502023/1‑140 (MQ=255)                                                       tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGc                                                                          >  1:882657/1‑140 (MQ=255)                                                       tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGc                                                                          >  2:992266/1‑140 (MQ=255)                                                       tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGc                                                                          >  1:649533/1‑140 (MQ=255)                                                       tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAAGGGGAATGGGCATTGGCTGGGCGCTACTTGAAGAGATGATCATCGATGc                                                                          >  2:448134/1‑140 (MQ=255)                                                       tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAAAGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGc                                                                          >  2:466161/1‑140 (MQ=255)                                                            aGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTaaaa                                                                     >  1:590438/1‑140 (MQ=255)                                                                 ccATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAgcggc                                                                >  1:95132/1‑140 (MQ=255)                                                                    tCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTg                                                             <  2:882657/140‑1 (MQ=255)                                                                         cGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCg                                                        <  1:737614/140‑1 (MQ=255)                                                                                 cAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGTTGCTAATAGCGGCCTGGTCCGTAACCCCa                                                >  1:456391/1‑140 (MQ=255)                                                                                  aaCGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAgcggcg                                                               >  1:739927/1‑124 (MQ=255)                                                                                  aaCGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAgcggcg                                                               <  2:739927/124‑1 (MQ=255)                                                                                  aaCGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCaa                                               >  2:761140/1‑140 (MQ=255)                                                                                      ttCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATctg                                           <  1:889425/140‑1 (MQ=255)                                                                                      ttCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGACGTGGTCCGTAACCCCAATctg                                           >  1:483942/1‑140 (MQ=255)                                                                                                aGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACa                                 >  2:551665/1‑140 (MQ=255)                                                                                                aGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACa                                 <  2:245559/140‑1 (MQ=255)                                                                                                 gggCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAGAAGCGGCCTGGTCCGTAACCCCAATCTGCTGGATTACaa                                >  2:372887/1‑140 (MQ=255)                                                                                                  ggCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACaaa                               >  2:136354/1‑140 (MQ=255)                                                                                                       atTCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGGTAAAAGCGCCGTAGTCGGTAACCCCGATCTGCTGGATTACAAAATGcc                          >  2:313945/1‑140 (MQ=255)                                                                                                         tCTTAATCCACTGCTGGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGa                        <  2:649533/140‑1 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCGTCGCTGCTACAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGa              >  2:244271/1‑140 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGAc                       >  1:117613/1‑131 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGAc                       <  2:117613/131‑1 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATg                   <  2:227218/135‑1 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATg                   >  1:227218/1‑135 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATg                   >  2:807552/1‑135 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATg                   <  1:807552/135‑1 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGa              >  1:571569/1‑140 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGa              >  2:210215/1‑140 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGa              >  1:178138/1‑140 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGa              >  2:976214/1‑140 (MQ=255)                                                                                                                   ctgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGAGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGGGGACCGTAACCCCAATCTCCTGAATTGCAACATGCCGACCATGCCGGa              >  2:224477/1‑140 (MQ=255)                                                                                                                    tgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGAt             >  1:538460/1‑140 (MQ=255)                                                                                                                    tgctgGCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAAATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGAt             >  1:738069/1‑140 (MQ=255)                                                                                                                        ggCAGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAAATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGATCTGc         <  2:738069/140‑1 (MQ=255)                                                                                                                           aGAAGGTCAGGCACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGATAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGATCTGCcac      <  1:613532/140‑1 (MQ=255)                                                                                                                               ggTCAGGCACACGGCGGAATGGGAATGGGCATTGACTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGATCTGCCACAACt  >  2:870488/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     AACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGACCATGCCGGATCTGCCACAACT  >  NC_000913/3002145‑3002410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑