Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,108,233	A→G	F308S (TTT→TCT)	speC ←	ornithine decarboxylase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,108,233	0	A	G	100.0%	203.5 / NA	57	F308S (TTT→TCT)	speC	ornithine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/34);  total (23/34)

TGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTC  >  NC_000913/3108097‑3108369                                                                                                                                         |                                                                                                                                         tgAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACa                                                                                                                                       <  2:737425/140‑1 (MQ=255)  gagaATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGTTAAATTGTTCATAAGCGACCCGCGCGGAATCAGACAg                                                                                                                                      >  1:264423/1‑140 (MQ=255)    gaATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTCTCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGaa                                                                                                                                    <  1:93710/140‑1 (MQ=255)       tCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGt                                                                                                                                 >  1:806162/1‑140 (MQ=255)       tCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCAATCAGCGGCATAAATTGTTCATAACCGACCCCCGCGGAATCAGACAGAATGt                                                                                                                                 <  2:956737/140‑1 (MQ=255)       tCCCGCCTGCTGTTTGTCCACCAACTGAGTCTCAATGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGt                                                                                                                                 <  2:325065/140‑1 (MQ=255)           gCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATc                                                                                                                             <  1:947476/140‑1 (MQ=255)           gCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATc                                                                                                                             >  1:241261/1‑140 (MQ=255)           gCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTCCATAACCTACCTACACGGAATGAGACAGAATGTAATc                                                                                                                             >  1:473832/1‑140 (MQ=255)              tgctgTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATcaca                                                                                                                          <  2:910346/140‑1 (MQ=255)                 tgTTTGTGCACCGACTGCGTCACAAAGATCCCCGGGTCGGTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAg                                                                                                                       <  2:429274/140‑1 (MQ=255)                    ttGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATg                                                                                                                    <  1:95083/140‑1 (MQ=255)                    ttGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATg                                                                                                                    <  2:78212/140‑1 (MQ=255)                    ttGTGCACCGACTGAATCACAATGATCCCGGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATg                                                                                                                    <  1:339474/140‑1 (MQ=255)                      gtgCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGcc                                                                                                                  <  1:123809/140‑1 (MQ=255)                      gtgCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGcc                                                                                                                  <  1:786828/140‑1 (MQ=255)                      gtgCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGcc                                                                                                                  <  2:629368/140‑1 (MQ=255)                            cGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACgg                                                                                                            <  2:185177/140‑1 (MQ=255)                            cGACTGAGTCACAAAGACCCCCCGATCGTTTTCGTTAAGCTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACgg                                                                                                            <  2:321713/140‑1 (MQ=255)                             gACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGt                                                                                                           >  1:718276/1‑140 (MQ=255)                                  aGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGa                                                                                                      >  1:908740/1‑140 (MQ=255)                                     cacaAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCa                                                                                                   <  2:908740/140‑1 (MQ=255)                                         aaGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTg                                                                                               <  1:46383/140‑1 (MQ=255)                                           gATCCCCGGATCGTTTTCGTTATGTTCTAACAGCAGCGGGGAGCTATCAGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGAc                                                                                             <  2:400886/140‑1 (MQ=255)                                             tCCCCGGATCGTTTTCTTTAAGTTCTAACATCACCGGCGTGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                           <  2:473832/140‑1 (MQ=255)                                             tCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                           <  1:980812/140‑1 (MQ=255)                                             tCCCCGGATCGTTTTCGTTAAGCTCTAACTGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                           <  1:277824/140‑1 (MQ=255)                                             tCCCCGGATCGTTTTCATTAAGCTCTAACAGAAGCAGCGCGCTATCCGACATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                           <  2:484183/140‑1 (MQ=255)                                             tCCCCGGATCGTTTTAGTTAAGTTCGAACAGCAGCGGTGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                           <  1:426693/140‑1 (MQ=255)                                              ccccGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATGACAGAGATGCCCGACGGTATCGATCACCTGACggg                                                                                          >  2:277824/1‑140 (MQ=255)                                               cccGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGc                                                                                         >  1:845895/1‑140 (MQ=255)                                               cccGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGc                                                                                         <  2:925486/140‑1 (MQ=255)                                                   gATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                           >  2:722755/1‑134 (MQ=255)                                                   gATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                           <  1:722755/134‑1 (MQ=255)                                                      cGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                           >  2:710125/1‑131 (MQ=255)                                                      cGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                           <  1:710125/131‑1 (MQ=255)                                                       gTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGa                                                                                 <  2:718276/140‑1 (MQ=255)                                                         tttCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACa                                                                               <  2:665801/140‑1 (MQ=255)                                                              ttAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGcc                                                                          <  2:89851/140‑1 (MQ=255)                                                                            cagcGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGTTATCGATCTCCTGACGGGCGTTATAAACAGGGCCGTCATAGGTTCCCa                                                            >  2:329155/1‑140 (MQ=255)                                                                            cagcGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACTTGACGGGAGTTATAGACAGTGGCGTCATAGGTTCCCa                                                            >  1:484614/1‑140 (MQ=255)                                                                            cagcGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCa                                                            >  1:176980/1‑140 (MQ=255)                                                                             agcGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAg                                                           >  1:22690/1‑140 (MQ=255)                                                                             agcGGCGAGCTATCCGCAATCATCGGGATAAATTGGTCATAACCGACCAACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAg                                                           <  2:405432/140‑1 (MQ=255)                                                                              gcggcgAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGc                                                          >  1:549790/1‑140 (MQ=255)                                                                               cggcgAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCt                                                         >  2:506213/1‑140 (MQ=255)                                                                               cggcgAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCt                                                         >  2:965663/1‑140 (MQ=255)                                                                               cggcgAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCt                                                         >  1:218744/1‑140 (MQ=255)                                                                               cggcgAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCt                                                         >  2:925347/1‑140 (MQ=255)                                                                                    aGCTATCCGCCATCATCGGGATAAATTGTACATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGGGTTATAGACAGGGCGGTCATAGGTTCCCAGCTGaata                                                    >  1:292080/1‑140 (MQ=255)                                                                                      cTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGCTATAGACGGTGCCGGCATAGGCTCCCAGCTGaataat                                                  >  1:325716/1‑140 (MQ=255)                                                                                        aTCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCg                                                <  2:549790/140‑1 (MQ=255)                                                                                           cGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCa                                             <  2:22690/140‑1 (MQ=255)                                                                                             ccATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAgg                                           <  2:241261/140‑1 (MQ=255)                                                                                                                ttCATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGt                        <  2:845895/140‑1 (MQ=255)                                                                                                                  cATAACCGACCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCg                      >  2:250463/1‑140 (MQ=255)                                                                                                                          aCCCACGCGGAATCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTc              >  1:726958/1‑140 (MQ=255)                                                                                                                                     aTCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGt   >  1:870911/1‑140 (MQ=255)                                                                                                                                      tCAGACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTc  <  1:925347/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTC  >  NC_000913/3108097‑3108369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑