Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,146,661 T→C K26R (AAA→AGA)  yghW ← DUF2623 domain‑containing protein YghW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,146,6610TC98.4% 189.7 / NA 61K26R (AAA→AGA) yghWDUF2623 domain‑containing protein YghW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (40/20);  minor base A (1/0);  total (41/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TACCATCTCTTTATCCAGTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACG  >  NC_000913/3146525‑3146793
                                                                                                                                        |                                                                                                                                    
tACCATCTCTTTATCCAGTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTg                                                                                                                                   <  1:690184/140‑1 (MQ=255)
    aTCTCTTTATCCAGTTCATAGCAGCCCGTTAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTcac                                                                                                                               <  1:327395/140‑1 (MQ=255)
    aTCTCTTTATCCAGTGCATAGCCGCTCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTcac                                                                                                                               <  1:455139/140‑1 (MQ=255)
       tctTTATCCAGTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACAtt                                                                                                                            >  1:130391/1‑140 (MQ=255)
        ctTTATCCAGTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTa                                                                                                                           >  2:224173/1‑140 (MQ=255)
            aTCCAGTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCg                                                                                                                       <  2:352944/140‑1 (MQ=255)
                aGTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACt                                                                                                                   >  1:505865/1‑140 (MQ=255)
                 gTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACGAAACCGCGCTTAAAATAGGCACAGAATCTTATCACATTAACCGCACTg                                                                                                                  >  2:411671/1‑140 (MQ=255)
                 gTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGTCACAGATTCTTGTCACATTAACCGCACTg                                                                                                                  >  1:440618/1‑140 (MQ=255)
                 gTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTg                                                                                                                  >  1:646369/1‑140 (MQ=255)
                 gTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTg                                                                                                                  >  1:205722/1‑140 (MQ=255)
                 gTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTg                                                                                                                  >  2:96400/1‑140 (MQ=255)
                 gTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTGATAATCGTCACGGAGTCTTGTGACATTAACCGCACTg                                                                                                                  >  1:287823/1‑140 (MQ=255)
                  tCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACATAACCGCGTATATTACCGGCACAGAACCTTGCAACATTAAACGCACTGt                                                                                                                 >  2:490876/1‑140 (MQ=255)
                  tCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTCTAATCGGAACATAATCTTGTCGCATTAACCGCACTGt                                                                                                                 >  1:310519/1‑140 (MQ=255)
                  tCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACATAAACTTGTCGCATTAACCGCACTGt                                                                                                                 >  1:431520/1‑140 (MQ=255)
                  tCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGt                                                                                                                 >  1:849705/1‑140 (MQ=255)
                  tCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGt                                                                                                                 >  2:895088/1‑140 (MQ=255)
                  tCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGt                                                                                                                 >  1:653978/1‑140 (MQ=255)
                   ccATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTc                                                                                                                >  1:801848/1‑140 (MQ=255)
                   ccATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTc                                                                                                                >  1:668387/1‑140 (MQ=255)
                      tAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGc                                                                                                             <  1:239574/140‑1 (MQ=255)
                       aGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTg                                                                                                                  <  2:181072/134‑1 (MQ=255)
                       aGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTg                                                                                                                  >  1:181072/1‑134 (MQ=255)
                       aGCGGCGCGTCAGAACACCCGCCTCCCAGACGCTAAGTTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCa                                                                                                            <  1:307173/140‑1 (MQ=255)
                            cgcgTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGTACACAATATTGTCACATTAACATCACTGTCGGCATGCGt                                                                                                       >  2:477287/1‑140 (MQ=255)
                            cgcgTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGGACAGAATATTGTCACAATAACCGCACTGTCGGCATGCGt                                                                                                       >  2:326929/1‑140 (MQ=255)
                            cgcgTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCAGAGAATCTAGTCACATTAACCGCACTGTCGGCATGCGt                                                                                                       >  1:288034/1‑140 (MQ=255)
                            cgcgTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGt                                                                                                       >  1:831341/1‑140 (MQ=255)
                              cgTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTg                                                                                                     >  1:60615/1‑140 (MQ=255)
                              cgTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTg                                                                                                     >  1:866232/1‑140 (MQ=255)
                              cgTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGGCTTTTCGTACATCCGGTGTGAGTAGTCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTg                                                                                                     >  1:689789/1‑140 (MQ=255)
                                              tCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCAt                                                                                     >  1:257168/1‑140 (MQ=255)
                                              tCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATAAACCGCACTGTCGGCACGCGTTACTTTTAATCCCGCCAt                                                                                     >  2:468475/1‑140 (MQ=255)
                                                   ggCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCg                                                                                         <  1:776628/131‑1 (MQ=255)
                                                   ggCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCg                                                                                         >  2:776628/1‑131 (MQ=255)
                                                      gcTAAGCTGGCGATCTCCGGTCTTTTCGTACGTCCTGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCTGCCATTAAGCCtt                                                                             <  2:387278/140‑1 (MQ=255)
                                                       cTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCttt                                                                            >  1:247437/1‑140 (MQ=255)
                                                         aaGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTAc                                                                          >  2:853432/1‑140 (MQ=255)
                                                            cTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCAAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCaa                                                                       <  1:775852/140‑1 (MQ=255)
                                                               gCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAAt                                                                    >  1:957322/1‑140 (MQ=255)
                                                                cGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATg                                                                   <  1:895088/140‑1 (MQ=255)
                                                                        ggTCTTTTCGTACATCCTGTGTCAGTAGCCTTATACAAAACCGCGTTTTTAATCGGCACAGAATCTTTTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCa                                                           <  2:310519/140‑1 (MQ=255)
                                                                        ggTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAACCCGCTTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCTTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCa                                                           <  2:895717/140‑1 (MQ=255)
                                                                        ggTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGTTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCa                                                           <  2:849705/140‑1 (MQ=255)
                                                                        ggTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCa                                                           <  1:96400/140‑1 (MQ=255)
                                                                              ttCGTACATCCGGTGTGGGTAGGCTAATACACACCTGCGCTTATAATCTGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTc                                                     <  2:287823/140‑1 (MQ=255)
                                                                              ttCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCaa                                                                       >  2:670530/1‑122 (MQ=255)
                                                                              ttCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCaa                                                                       <  1:670530/122‑1 (MQ=255)
                                                                                       ccGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTAtt                                                             >  1:221046/1‑123 (MQ=255)
                                                                                       ccGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTAtt                                                             <  2:221046/123‑1 (MQ=255)
                                                                                       ccGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTc                                            <  1:514334/140‑1 (MQ=255)
                                                                                       ccGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTc                                            <  2:653978/140‑1 (MQ=255)
                                                                                        cGGTGTGAGTAGCCTAATACAAACCCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAACCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCg                                           <  2:60615/140‑1 (MQ=255)
                                                                                        cGGTGTGAGTACCCTAATACAAACCCGCTTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCg                                           <  1:65426/140‑1 (MQ=255)
                                                                                          gtgtGAGTAGCCTAATACAAACCCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCt                                         <  1:722266/140‑1 (MQ=255)
                                                                                          gtgtGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCt                                         <  2:957322/140‑1 (MQ=255)
                                                                                            gtgAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGTTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtat                                       <  2:205722/140‑1 (MQ=255)
                                                                                               aGTAGCCTAATACAACACCGCTTTTGTAATCGGCACAAAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatata                                    <  1:490876/140‑1 (MQ=255)
                                                                                               aGTAGCCTAATACAAACCCGCTTTTATAATCCGCAAAGATTCTTGTCATATTAACCGCACTGTCGGCATGCGTTGTTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatata                                    <  2:306668/140‑1 (MQ=255)
                                                                                               aGTAGCCTAATACAAACCCGCGTTTATAATCGGCACAGAATCTTGTCACATTACCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatata                                    <  2:33426/140‑1 (MQ=255)
                                                                                               aGTAGCCTAATACAAACCCGCGTTTATAATCGGCAAAGAATCTTGTCACATAACCCGCACTGTGGGCAGGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatata                                    <  2:228410/140‑1 (MQ=255)
                                                                                               aGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGTTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatata                                    <  2:831341/140‑1 (MQ=255)
                                                                                               aGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatata                                    >  1:780741/1‑140 (MQ=255)
                                                                                               aGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatata                                    <  2:646369/140‑1 (MQ=255)
                                                                                               aGTACCCTAATAAAAATTCGCTTTTATAATCACTAAAGAATCTTGTCACATAACCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatata                                    <  2:440618/140‑1 (MQ=255)
                                                                                               aGTAACCTAATACAAACCCGCTTTTATATTCGGCCGAGAATCTTTTCACTTTACCCGCCCTGTCGCCATGGTTTGTTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtatata                                    <  2:801848/140‑1 (MQ=255)
                                                                                                 tAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTGTTCATCATTCACCGCATTCGCTATATAtt                                  >  1:306668/1‑140 (MQ=255)
                                                                                                   gCCTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGt                                >  1:810015/1‑140 (MQ=255)
                                                                                                    ccTAATACAAAACCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTc                               >  2:902991/1‑140 (MQ=255)
                                                                                                       aaTACAAACCCGCGTTTATAATTGGTACATAATCTTGGCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCata                            <  1:411671/140‑1 (MQ=255)
                                                                                                       aaTACAAACCCGCGTTTAAAACCGGCAAAGACTCTCGTCTCATTACCCCCACTGTCGGCATGCGTTGCTTTTAACCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCata                            <  2:288034/140‑1 (MQ=255)
                                                                                                              acccGCGTTTATAACCGGCACAGAATCTTGTCAAATTACCCGCACTGTGGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCg                     <  2:130391/138‑1 (MQ=255)
                                                                                                              aaCCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCg                     >  1:555475/1‑140 (MQ=255)
                                                                                                              aaCCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCg                     >  2:735131/1‑140 (MQ=255)
                                                                                                              aaCCGCGTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCg                     >  1:673952/1‑140 (MQ=255)
                                                                                                                ccGCTTTTATAATCGTCACAGACTCATGTAACATTACCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGat                   <  1:477287/140‑1 (MQ=255)
                                                                                                                  gcgTTTATAATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGatat                 >  1:190821/1‑140 (MQ=255)
                                                                                                                        ataATCGGCACAGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAgg           >  2:888260/1‑140 (MQ=255)
                                                                                                                                 acaGAATCTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACg  >  2:908111/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TACCATCTCTTTATCCAGTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACG  >  NC_000913/3146525‑3146793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑