Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,244,143	T→C	Y200C (TAC→TGC)	uxaC ←	D‑glucoronate/D‑galacturonate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,244,143	0	T	C	100.0%	180.9 / NA	51	Y200C (TAC→TGC)	uxaC	D‑glucoronate/D‑galacturonate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/23);  total (28/23)

CACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGTAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGG  >  NC_000913/3244010‑3244272                                                                                                                                      |                                                                                                                                  caccacATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGt                                                                                                                             <  1:622907/140‑1 (MQ=255) caccacATCCAGCGCGTGGTCACACACTTTACAGCAGTGAGCGGCGAACTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGt                                                                                                                             <  2:287836/140‑1 (MQ=255)        tCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGt                                                                                                                      <  2:954677/140‑1 (MQ=255)         ccAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGACCATCTGCGCCATACAGTCGTTAAAGGTc                                                                                                                     >  1:287836/1‑140 (MQ=255)           aGCGGGTGGTCAGACATATTACCGCCGTGAGTGGCCAAGCGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGc                                                                                                                   <  2:345734/140‑1 (MQ=255)           aGCGCGTGGTCAGACCCTTTACCGCCGTGGGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGc                                                                                                                   <  1:411709/140‑1 (MQ=255)           aGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGc                                                                                                                   <  2:1012224/140‑1 (MQ=255)           aGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGc                                                                                                                   <  2:560563/140‑1 (MQ=255)            gcgcGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGcc                                                                                                                  >  2:784295/1‑140 (MQ=255)             cgtgTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCt                                                                                                                 >  2:972625/4‑140 (MQ=255)                gTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGtt                                                                                                              <  1:972625/140‑1 (MQ=255)                 tGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTc                                                                                                             >  1:47733/1‑140 (MQ=255)                  ggTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCg                                                                                                            <  2:47733/140‑1 (MQ=255)                  gatCAGTCTCTTTCCAGCCGAGAGCAACGAAGTGATGCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAAAATAACGGAGAATGTCGGTATCGTAATCTTAGCGAAGCGTCGCGATGCAGTCGTTAAAGGTCGCCTGTTCg                                                                                                            <  2:1005166/138‑1 (MQ=255)                              tACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCt                                                                                                >  1:1017646/1‑140 (MQ=255)                                      tGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCgg                                                                                        >  1:512922/1‑140 (MQ=255)                                      tGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCgg                                                                                        >  1:614972/1‑140 (MQ=255)                                      tGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCgg                                                                                        >  2:866238/1‑140 (MQ=255)                                        aGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTCAAGGTCGGCTGCTCGATGTTGCAGGCTTTGTCCGGAc                                                                                      >  1:475803/1‑140 (MQ=255)                                        aGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGAc                                                                                      >  1:583486/1‑140 (MQ=255)                                        aGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGAc                                                                                      >  1:615378/1‑140 (MQ=255)                                             cgAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAg                                                                                 >  1:703856/1‑140 (MQ=255)                                                            gTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTg                                                                  >  2:166538/1‑140 (MQ=255)                                                             tttGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGa                                                                 >  2:677293/1‑140 (MQ=255)                                                             tttGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGa                                                                 <  2:794996/140‑1 (MQ=255)                                                              ttGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGAt                                                                <  1:373696/140‑1 (MQ=255)                                                               tGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATg                                                               >  2:963608/1‑140 (MQ=255)                                                               tGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGGTCGATGTTAAAGTCTTTGTCCGGACACCAGCTCGGCAGCACTTTGATg                                                               >  2:353416/1‑140 (MQ=255)                                                                           tttGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCATCTCGGCAGCACTTTGATGGTGAAAGAGCCg                                                   >  2:185943/1‑140 (MQ=255)                                                                           tttGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCg                                                   >  2:169549/1‑140 (MQ=255)                                                                              gCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCt                                                <  1:820400/140‑1 (MQ=255)                                                                               cAGGTCAGCAAAGCGGCGAATGTCGTTATCGGAAACTGCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCtt                                               <  1:390002/140‑1 (MQ=255)                                                                                aGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCttt                                              >  1:869265/1‑140 (MQ=255)                                                                                     aGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGa                                         <  2:526167/140‑1 (MQ=255)                                                                                      gCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGAt                                        <  2:951230/140‑1 (MQ=255)                                                                                        aaaGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGAtct                                      <  1:784295/140‑1 (MQ=255)                                                                                          aGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGTCAGCTCGGTAGCCCTTTGATGGTGAAAGAGACGTCTTTGGCGATctct                                    >  2:415648/1‑140 (MQ=255)                                                                                            cggcgAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGc                                  >  1:182252/1‑140 (MQ=255)                                                                                            cggcgAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGc                                  >  2:723756/1‑140 (MQ=255)                                                                                            cggcgAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGc                                  >  1:192166/1‑140 (MQ=255)                                                                                             ggcgAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCg                                 <  2:614972/140‑1 (MQ=255)                                                                                               cgAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTCTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTg                               <  2:589459/140‑1 (MQ=255)                                                                                                  aTGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATg                            <  2:1017646/140‑1 (MQ=255)                                                                                                  aTGTCGGTATCGGAAACCTCGCGCAGCTTCGGCATTCAGCCGTTAAAGTTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATg                            <  2:334332/140‑1 (MQ=255)                                                                                                   tGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGc                           <  1:866238/140‑1 (MQ=255)                                                                                                              gAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAatc                >  2:610090/1‑140 (MQ=255)                                                                                                              gAAACTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAatc                >  2:962618/1‑140 (MQ=255)                                                                                                                 aCTTCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAatcgat             <  1:185943/140‑1 (MQ=255)                                                                                                                   ttCGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAatcgatcg           >  1:150575/1‑140 (MQ=255)                                                                                                                     cGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGTCGATCTCTGCGTGATGCTCCAGAGAATCGATCggg         >  2:347144/1‑140 (MQ=255)                                                                                                                     cGCCCAGCTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCggg         >  1:614856/1‑140 (MQ=255)                                                                                                                            cTTCGCCATGCAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCgg  <  2:41070/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  CACCACATCCAGCGCGTGGTCAGACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTTTGCAGGTCAGCAAAGCGGCGAATGTCGGTATCGGAAACTTCGCCCAGCTTCGCCATGTAGTCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGG  >  NC_000913/3244010‑3244272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑