Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,305,770	G→A	G16G (GGC→GGT)	mtr ←	tryptophan:H(+) symporter Mtr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,305,770	0	G	A	100.0%	273.5 / NA	84	G16G (GGC→GGT)	mtr	tryptophan:H(+) symporter Mtr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/44);  total (40/44)

TCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGAC  >  NC_000913/3305637‑3305895                                                                                                                                      |                                                                                                                              tcaAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCa                                                                                                                         <  2:55587/140‑1 (MQ=255)        ggAATGAAAAATACAAAACCAAGAAAAGATCAGCGCCGCCATTGACCAGAAAATCCACGCCCCGGACATAACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCg                                                                                                                  <  2:165574/140‑1 (MQ=255)          aaTGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGAc                                                                                                                >  2:665460/1‑140 (MQ=255)             gcagcaTACAGAACCAGGTAAAGACCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGt                                                                                                             <  2:256247/140‑1 (MQ=255)               agcaTACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGa                                                                                                           >  2:627020/1‑140 (MQ=255)                  aTACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGt                                                                                                        >  2:187078/1‑140 (MQ=255)                   tACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGtt                                                                                                       >  2:982171/1‑140 (MQ=255)                      aGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTgg                                                                                                    <  1:143622/140‑1 (MQ=255)                      aGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGACAATCACCACACCGCCAAGCAGCGACGGTGACGTTTgg                                                                                                    >  1:418736/1‑140 (MQ=255)                       gAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTggg                                                                                                   >  1:509119/1‑140 (MQ=255)                        aaCCAGGTAAAGATCTGCGCCGCCATTGAGCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGggt                                                                                                  <  1:318061/140‑1 (MQ=255)                        aaCCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGggt                                                                                                  <  1:835454/140‑1 (MQ=255)                         aCCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGCCATGACCACTGGCAGAGAAAACATCCCTGCCCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGggtg                                                                                                 <  2:962357/140‑1 (MQ=255)                         aCCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGggtg                                                                                                 <  2:622065/140‑1 (MQ=255)                         aCCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGggtg                                                                                                 <  1:79376/140‑1 (MQ=255)                           cAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCAGCCGATAATCACCACAACGCCAAGCTGCGAGGGTGACGTTTGggtggt                                                                                               >  2:318061/1‑140 (MQ=255)                             ggTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACGACACCGCGAAGGAGTGACGGTAACGTTTGggtggtgg                                                                                             >  1:312217/1‑140 (MQ=255)                             ggTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGggtggtgg                                                                                             >  2:847428/1‑140 (MQ=255)                             ggTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGggtggtgg                                                                                             >  1:230877/1‑140 (MQ=255)                             ggTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGggtggtgg                                                                                             >  2:916223/1‑140 (MQ=255)                              gTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGggtggtggt                                                                                            <  2:620179/140‑1 (MQ=255)                               tAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGtt                                                                                           >  1:145341/1‑140 (MQ=255)                                  aGATCAGCGCCGCCATTGACCAGAACAACGACGCCCCCGACATGACCACTGGCAGAGAAAACATCCCTCCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAgt                                                                                        <  1:404091/140‑1 (MQ=255)                                       aGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAGGCAGCGATGGTCACGTTTGGGAGGTGGTTAGTGTTGc                                                                                   >  1:493833/1‑140 (MQ=255)                                        gcgcCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGcc                                                                                  >  2:850363/1‑140 (MQ=255)                                        gcgcCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGcc                                                                                  >  2:839029/1‑140 (MQ=255)                                        gcgcCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGcc                                                                                  >  2:639754/1‑140 (MQ=255)                                         cgccgcCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCa                                                                                 >  1:102033/1‑140 (MQ=255)                                          gccgccATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCAt                                                                                <  1:847428/140‑1 (MQ=255)                                            cgccATTGACCAGAAAACCCACACCCCGGACCTGAACACCGGCAGAGAGAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGa                                                                              <  2:416869/140‑1 (MQ=255)                                             gccATTGACCAGAAAACGCACGCCCCGGACATGACAACTGGCAGAGAACACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAg                                                                             <  2:321573/140‑1 (MQ=255)                                             gccATTGACCAGAAAAACCACGCCCCGGACATGACCACTCGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAg                                                                             <  2:365235/140‑1 (MQ=255)                                             cccATTGACCAGAAAACTCACGTCCCGGACACGACCACTCGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAg                                                                             <  2:299662/139‑1 (MQ=255)                                             accATTGACCAAAAAAACCACCCCCCGAACATGACCTCTCGCAGAGAAAACATCCCTGCGCCAAAAATGGGGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAg                                                                             <  2:145341/139‑1 (MQ=255)                                              ccATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAgg                                                                            >  2:556147/1‑140 (MQ=255)                                              ccATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCCGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAgg                                                                            >  2:51060/1‑140 (MQ=255)                                                aTTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTGTGGATGATGGTTACTGTTGCCATGAGGGc                                                                          >  1:299662/1‑140 (MQ=255)                                                  tGACCAGAAAAACCATGCCCCGCACATGACCACTGGCCGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCtt                                                                        <  1:264280/140‑1 (MQ=255)                                                   gACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATg                                                                               >  2:826495/1‑132 (MQ=255)                                                   gACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATg                                                                               <  1:826495/132‑1 (MQ=255)                                                   gACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTtc                                                                       >  2:993366/1‑140 (MQ=255)                                                       aGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTtctctc                                                                   <  2:753261/140‑1 (MQ=255)                                                         aaaaaCCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCa                                                                 <  1:665460/140‑1 (MQ=255)                                                                         aCATGACCACTGGCAAAGAAAATATACCTTCGCCAATCATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGa                                                 <  1:264690/140‑1 (MQ=255)                                                                            tGACCACTGGCAGAGAAAACATTCCTGCGTCAATAATCGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTg                                              <  1:500174/140‑1 (MQ=255)                                                                            tGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTg                                              <  1:627020/140‑1 (MQ=255)                                                                              aCCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCGTGAGGGCTTCTGTCGAGTGAAGAATAGTGCGACTGCg                                            >  1:346836/1‑140 (MQ=255)                                                                                  cTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGtgttgt                                        >  1:212944/1‑140 (MQ=255)                                                                                  cTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCgttgt                                        >  1:427105/1‑140 (MQ=255)                                                                                  cTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTG‑AAAATAGTGCGACTGCgttgtt                                       >  2:653455/1‑140 (MQ=255)                                                                                   gagacaggaAAACATCCTTGCGCCAATAATGCTGCGGCCCATAATCACGACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCgttgtt                                       <  2:364406/133‑1 (MQ=255)                                                                                   cggCAGAAAAAACATCCCTGCACCAATAGTGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCgttgtt                                       <  2:230877/139‑1 (MQ=255)                                                                                     gCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTAt                                     >  2:654896/1‑140 (MQ=255)                                                                                         agaAAACATCCCTGCGCCAATATTGGTGCCGCCGAAAATGACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCAt                                 <  2:312217/140‑1 (MQ=255)                                                                                         agaAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCAt                                 <  1:700778/140‑1 (MQ=255)                                                                                         agaAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCAt                                 <  2:611258/140‑1 (MQ=255)                                                                                           aaaaCATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTg                               <  2:958741/140‑1 (MQ=255)                                                                                           aaaaCATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTg                               <  1:91320/140‑1 (MQ=255)                                                                                             aaCATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCa                             <  1:74924/140‑1 (MQ=255)                                                                                             aaCATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCa                             <  2:427105/140‑1 (MQ=255)                                                                                             aaCATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCa                             <  1:716632/140‑1 (MQ=255)                                                                                             aaCATCCCCGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCa                             <  1:993366/140‑1 (MQ=255)                                                                                              aCATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCAc                            <  1:452762/140‑1 (MQ=255)                                                                                               cATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACt                           <  2:555479/140‑1 (MQ=255)                                                                                                   ccTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTAc                       <  1:916223/140‑1 (MQ=255)                                                                                                   ccTGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTAc                       <  1:716240/140‑1 (MQ=255)                                                                                                    cTGCGCCAATAATGGCGCCGCCGATTATCACCACACCTCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTAcc                      <  1:289364/140‑1 (MQ=255)                                                                                                     tGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAGATAGTGCAACTGCGCTGTTATGCATTGCAcctgtctc                     >  1:364406/1‑133 (MQ=255)                                                                                                     tGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCa                             <  2:207268/132‑1 (MQ=255)                                                                                                     tGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCa                             >  1:752306/1‑132 (MQ=255)                                                                                                     tGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCa                             <  2:752306/132‑1 (MQ=255)                                                                                                     tGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCa                             >  1:207268/1‑132 (MQ=255)                                                                                                     tGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCa                     >  1:113631/1‑140 (MQ=255)                                                                                                     tGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCa                     >  1:711437/1‑140 (MQ=255)                                                                                                     tGCGCCAATAATGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTATCCAGTGAAAAAGAGTACGACTGCGTTGTTGTGCATTGCACTGTACCa                     >  2:289364/1‑140 (MQ=255)                                                                                                             taatGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGa             <  1:187078/140‑1 (MQ=255)                                                                                                             taatGGTGCCGCCGATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTACGACTGCTTTGTTATGAATTGAACTGTACCAGTACACGa             >  2:393612/1‑140 (MQ=255)                                                                                                                     ccgccgATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACaaaa     <  1:654896/140‑1 (MQ=255)                                                                                                                      cgccgATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAg    >  2:756672/1‑140 (MQ=255)                                                                                                                      cgccgATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAg    >  2:926895/1‑140 (MQ=255)                                                                                                                      cgccgATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAg    >  1:24009/1‑140 (MQ=255)                                                                                                                       gccgATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGa   >  1:68089/1‑140 (MQ=255)                                                                                                                       gccgATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGa   >  2:741767/1‑140 (MQ=255)                                                                                                                        ccgATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGAc  >  1:63637/1‑140 (MQ=255)                                                                                                                        ccgATAATCACCACACCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGAc  <  1:741767/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                              TCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGAC  >  NC_000913/3305637‑3305895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑