Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,326,822	T→C	L38L (CTA→CTG)	ftsH ←	ATP‑dependent zinc metalloprotease FtsH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,326,822	0	T	C	100.0%	237.5 / NA	65	L38L (CTA→CTG)	ftsH	ATP‑dependent zinc metalloprotease FtsH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/27);  total (38/27)

TTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGTAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACCTCTT  >  NC_000913/3326685‑3326958                                                                                                                                          |                                                                                                                                         ttGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAg                                                                                                                                        >  2:42839/1‑140 (MQ=255)   ggTCAACAGGTTATCCATTAATGTCGGATCCTGAACCGGGATGTAAGTTGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGa                                                                                                                                      <  2:392426/140‑1 (MQ=255)      cAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAgg                                                                                                                                   <  2:892082/140‑1 (MQ=255)        aCAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTa                                                                                                                                 <  1:789264/140‑1 (MQ=255)          aGGTTATCCAGTAATGTCGGATCCTTAACCGGAATGTGAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTaga                                                                                                                               <  1:499638/140‑1 (MQ=255)                      aaTTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCtt                                                                                                                   >  2:217397/1‑140 (MQ=255)                        tttCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTAc                                                                                                                 >  1:16893/1‑140 (MQ=255)                          tCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACgg                                                                                                               >  2:524805/1‑140 (MQ=255)                          tCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACATGGTCGTTATTGCCCTCTTGCAGGAAGGTAGAGTAATCCACCTTACgg                                                                                                               >  2:351863/1‑140 (MQ=255)                               tCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGTAAGGTAGAGTAATACACCTTACGGCCAtt                                                                                                          >  1:277346/1‑140 (MQ=255)                                   gAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGAc                                                                                                      >  2:48941/1‑140 (MQ=255)                                    aaCCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACt                                                                                                     >  1:911094/1‑140 (MQ=255)                                        ggAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCt                                                                                                 <  2:108959/140‑1 (MQ=255)                                          aaTGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTgg                                                                                               >  2:529265/1‑140 (MQ=255)                                              tAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGccc                                                                                           >  2:917486/1‑140 (MQ=255)                                                    gTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCa                                                                                          >  2:885063/1‑135 (MQ=255)                                                    gTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCa                                                                                          <  1:885063/135‑1 (MQ=255)                                                       taACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTg                                                                                  >  1:255525/1‑140 (MQ=255)                                                        aaCGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCATCTTACGGCCATTAGTCTCGCTGGGCCCAAAGCTCTgg                                                                                 >  1:463433/1‑140 (MQ=255)                                                         aCGGTTACTATCTTTCTCGGTATCGTTGATTACACCTCCCTTGATACGTGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGa                                                                                <  2:277346/140‑1 (MQ=255)                                                          cGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCCTACGGCCGTTAAACCCGCTGGTCCCAAAGCTCTGGaa                                                                               >  1:398745/1‑140 (MQ=255)                                                               aCTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTg                                                                          >  1:15176/1‑140 (MQ=255)                                                               aCTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTg                                                                          >  2:110615/1‑140 (MQ=255)                                                               aCTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTg                                                                          >  1:995128/1‑140 (MQ=255)                                                               aCTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTg                                                                          >  2:608378/1‑140 (MQ=255)                                                               aCTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTg                                                                          >  1:88273/1‑140 (MQ=255)                                                               aCTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTg                                                                          >  1:779542/1‑140 (MQ=255)                                                                 tATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGAc                                                                        <  2:936885/140‑1 (MQ=255)                                                                 tATCTTTCATGGTAACGTTGATTTCACGTCCGTCGCTACCCGCTTCACCAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGAc                                                                        <  2:485798/140‑1 (MQ=255)                                                                           ggTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACaa                                                              <  2:16893/140‑1 (MQ=255)                                                                           ggTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACaa                                                              >  2:733892/1‑140 (MQ=255)                                                                               aCGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGc                                                          >  2:853323/1‑140 (MQ=255)                                                                               aCGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGc                                                          >  2:83491/1‑140 (MQ=255)                                                                               aCGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCGGGGGCCAAAGCTCTGGGATACAGACATCAGCACAACGGc                                                          >  2:277149/1‑140 (MQ=255)                                                                                cGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCa                                                         >  2:527022/1‑140 (MQ=255)                                                                                 gTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCaa                                                        >  2:722139/1‑140 (MQ=255)                                                                                    gATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGa                                                     <  2:15176/140‑1 (MQ=255)                                                                                    gATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGa                                                     <  2:88273/140‑1 (MQ=255)                                                                                    gATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGa                                                     <  2:730934/140‑1 (MQ=255)                                                                                     aTTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTTACATCAGCACAACGGCAATGAc                                                    >  1:770453/1‑140 (MQ=255)                                                                                       ttCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGAcca                                                  >  1:664828/1‑140 (MQ=255)                                                                                           cGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGAccagcca                                              <  2:995128/140‑1 (MQ=255)                                                                                           cGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGAccagcca                                              <  2:255525/140‑1 (MQ=255)                                                                                           cGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGAccagcca                                              <  1:529265/140‑1 (MQ=255)                                                                                               cGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGt                                          >  2:616443/1‑140 (MQ=255)                                                                                                      aCGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGAccagcca                                              <  1:510217/129‑1 (MQ=255)                                                                                                      aCGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGAccagcca                                              >  2:510217/1‑129 (MQ=255)                                                                                                      aCGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGt                                   <  1:547932/140‑1 (MQ=255)                                                                                                                        gTCGTTATTGACCTCTTGCAGGAAGGTAGCGTAATCCCCCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCa                 <  1:277149/140‑1 (MQ=255)                                                                                                                        gTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTTACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCa                 <  2:770453/140‑1 (MQ=255)                                                                                                                        gTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCa                 >  1:947803/1‑140 (MQ=255)                                                                                                                        gTCGTTATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCa                 <  1:83491/140‑1 (MQ=255)                                                                                                                             tATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGa            >  1:981887/1‑140 (MQ=255)                                                                                                                             tATTGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGa            >  2:642976/1‑140 (MQ=255)                                                                                                                               ttGACCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGAtt          <  2:664828/140‑1 (MQ=255)                                                                                                                                  aCCTCTTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGCTTTTTCGCCATGTCACTCAAGGGATTAAc       >  1:462850/1‑140 (MQ=255)                                                                                                                                   ccTCTTGCAGGAAGTAAGAGTAATCC‑CCCTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACct     <  1:841491/140‑1 (MQ=255)                                                                                                                                    ctctTGCAGGAAGGTAGAGTAATCCACCTTGCGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACct     <  1:616443/140‑1 (MQ=255)                                                                                                                                    ctctTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGGCCAGCCAGGGTAGTAGCTTTTTCGCGATGTGACTCAAGGGATTAACct     >  2:275432/1‑140 (MQ=255)                                                                                                                                    ctctTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACct     <  1:110615/140‑1 (MQ=255)                                                                                                                                    ctctTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACct     <  1:42839/140‑1 (MQ=255)                                                                                                                                    ctctTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACct     <  2:911094/140‑1 (MQ=255)                                                                                                                                    ctctTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCAATGTCACTCAAGGGATTAACct     >  2:316216/1‑140 (MQ=255)                                                                                                                                    ctctTGCAGGAAGGTACGGTAATCCACCTTACGACGATTTGACTCGCTAGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACct     <  2:449299/140‑1 (MQ=255)                                                                                                                                      ctTGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACctct   >  1:524337/1‑140 (MQ=255)                                                                                                                                       ttGCAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACCTCtt  >  2:252022/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCGCTTCACGAACCTGGTCGTTATTGACCTCTTGTAGGAAGGTAGAGTAATCCACCTTACGGCCATTAGACTCGCTGGGCCCAAAGCTCTGGAATACTGACATCAGCACAACGGCAATGACCAGCCAGAGTATTAGGTTTTTCGCCATGTCACTCAAGGGATTAACCTCTT  >  NC_000913/3326685‑3326958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑