Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,399,078	A→G	L301L (TTG→CTG)	mreC ←	cell shape determining protein MreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,399,078	0	A	G	100.0%	167.2 / NA	47	L301L (TTG→CTG)	mreC	cell shape determining protein MreC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/24);  total (23/24)

AGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGA  >  NC_000913/3398943‑3399214                                                                                                                                         |                                                                                                                                         agCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTAcc                                                                                                                                      >  2:91285/1‑140 (MQ=255) agCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTAcc                                                                                                                                      >  2:1001991/1‑140 (MQ=255)  gCGATTAGCAGCAGGGTGTGTCCGCTCAGAAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCt                                                                                                                                     <  1:358102/140‑1 (MQ=255)      ttAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTACCTTTGGCACCATCGCGACTGGCGAAGGCAGTACCTGCgg                                                                                                                                 >  2:358102/1‑140 (MQ=255)       tAGCAGCAGGCTGTGTCGGCGCTGCAGGCGCCGTAGCTGTATTTCCTGCCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGc                                                                                                                                <  2:416084/140‑1 (MQ=255)       tAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGc                                                                                                                                <  1:887962/140‑1 (MQ=255)        agcagcAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGc                                                                                                                                <  1:256374/140‑1 (MQ=255)          cagcagGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGcatc                                                                                                                             <  1:797044/140‑1 (MQ=255)            gcagGCTGTGTCGGCGCAGCAGTCGCAGTAGATGCGTTTGCTGTCGCCCGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGcatcat                                                                                                                           <  2:408777/140‑1 (MQ=255)             cagGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCatcatc                                                                                                                          <  2:175884/140‑1 (MQ=255)             cagGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCatcatc                                                                                                                          >  1:985554/1‑140 (MQ=255)             cagGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCatcatc                                                                                                                          <  2:662288/140‑1 (MQ=255)                    tgtCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCa                                                                                                                   >  2:927307/1‑140 (MQ=255)                    tgtCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCATCTGGCGAAGGAAGGACCTGCGGCATCATCTGCATCa                                                                                                                   >  1:338830/1‑140 (MQ=255)                      tCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGa                                                                                                                 <  1:600837/140‑1 (MQ=255)                       cGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGAc                                                                                                                >  1:558251/1‑140 (MQ=255)                       cGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGAc                                                                                                                >  2:536302/1‑140 (MQ=255)                       cGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGAc                                                                                                                <  1:192706/140‑1 (MQ=255)                          cgcAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGtt                                                                                                             >  2:705726/1‑140 (MQ=255)                          cgcAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGtt                                                                                                             >  2:784017/1‑140 (MQ=255)                                agGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAg                                                                                                       <  2:795924/140‑1 (MQ=255)                                  gcgcAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCa                                                                                                     <  2:45424/140‑1 (MQ=255)                                   cgcAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCCGTAACTGGGGCATCATCTGCATCAGACGTTCATTAGCaa                                                                                                    >  2:341241/1‑140 (MQ=255)                                                    cTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTcc                                                                                   >  1:18731/1‑140 (MQ=255)                                                    cTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTACATCAGACGTTAATGAGCAACACGATGCACCTCTTcc                                                                                   >  1:295585/1‑140 (MQ=255)                                                     tGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACATTCATTCGCAACATGATGCACCTCTTCCg                                                                                  >  1:355456/1‑140 (MQ=255)                                                      gTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGACATCATCTGCATGAGAGGTTCATTATCAACACGATGCACCTCTTCCgg                                                                                 >  2:322833/1‑140 (MQ=255)                                                            ggTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCAt                                                                           <  1:148824/140‑1 (MQ=255)                                                                 ctgcGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTAAGGTAACTTTGGTCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGt                                                                      <  2:306807/140‑1 (MQ=255)                                                                      gAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAgcg                                                                 <  2:126817/140‑1 (MQ=255)                                                                      gAGTCGGCTGAGCGAT‑CACCGTTGCCGGTTCTGGTAACTTTGGCCCCAACGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAgcg                                                                 <  2:395756/140‑1 (MQ=255)                                                                        gTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGcc                                                               >  2:60760/1‑140 (MQ=255)                                                                                        cccGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGcccc                                                >  1:727560/1‑140 (MQ=255)                                                                                          cGTTGCCGGTTCAGGCAACTTTGGCCCCAGCGCGTCTTGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGcccccc                                              <  1:341241/140‑1 (MQ=255)                                                                                                cGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGAt                                                       >  1:1005603/1‑125 (MQ=255)                                                                                                cGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGAt                                                       <  2:1005603/125‑1 (MQ=255)                                                                                                 ggTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCAcagcag                                       <  1:784017/140‑1 (MQ=255)                                                                                                  gTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCAcagcagc                                      <  1:240934/140‑1 (MQ=255)                                                                                                             tttGGCCCCATCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGGTCTCCCACCCACATCAGCAGCAGATAACg                           >  1:500306/1‑140 (MQ=255)                                                                                                               tagCCCCATCCCGTCTGGCGTATGCAGTACCTGCGTCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCTTCATCTGGTTAGAGACGTCAAGCTCTGCCCCACACATCAGCAGCAGATAACgta                         >  2:452821/3‑138 (MQ=255)                                                                                                                      aTCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTg                  >  1:731709/1‑140 (MQ=255)                                                                                                                      aTCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTg                  >  2:141919/1‑140 (MQ=255)                                                                                                                      aTCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTg                  >  1:211702/1‑140 (MQ=255)                                                                                                                      aTCGCGTCTGGCGAAGGCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTg                  >  2:19832/1‑140 (MQ=255)                                                                                                                        cgcgTCTGGCGAAGGCAGTACCAGCAGCATCATCTGCCTCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCa                <  1:322833/140‑1 (MQ=255)                                                                                                                           gTCTGGCGAAGGCAGTAGCTGCTGCATCATCGGCAGCAGCCGTTCATTCGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGcc             <  2:335447/140‑1 (MQ=255)                                                                                                                           gTCTGGCGAAGGCAGTACCTGCCGCATCATCTGCATCAGGCGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGcc             <  2:384293/140‑1 (MQ=255)                                                                                                                                      gCAGTACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGa  <  2:18731/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGAT‑CCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGA  >  NC_000913/3398943‑3399214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑