Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,549	C→T	M263I (ATG→ATA)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,549	0	C	T	100.0%	162.7 / NA	50	M263I (ATG→ATA)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/25);  total (25/25)

TCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCA  >  NC_000913/67415‑67685                                                                                                                                       |                                                                                                                                         tCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATccc                                                                                                                                     <  2:603433/140‑1 (MQ=255)   gCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCa                                                                                                                                   >  2:597157/1‑140 (MQ=255)     cTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGc                                                                                                                                 >  1:45877/1‑140 (MQ=255)                     gTAACCCTGCTGCGTCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGAAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCtt                                                                                                                 <  2:126147/140‑1 (MQ=255)                             gctgcATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACg                                                                                                         >  2:871370/1‑140 (MQ=255)                             gctgcATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACg                                                                                                         >  2:62437/1‑140 (MQ=255)                             gctgcATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACg                                                                                                         >  2:112467/1‑140 (MQ=255)                              ctgcATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAACCGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGt                                                                                                        <  1:862887/140‑1 (MQ=255)                                gcATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCTAGGAAACGGTTTATCCTCAGCTCAAGACGCACCGCTTCCAGCACGTTc                                                                                                      >  2:291486/1‑140 (MQ=255)                                   tCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTgc                                                                                                   >  1:634569/1‑139 (MQ=255)                                                   cAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGa                                                                                   <  1:597157/140‑1 (MQ=255)                                                     gACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGAtt                                                                                 >  1:114847/1‑140 (MQ=255)                                                     gACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGAtt                                                                                 >  1:6167/1‑140 (MQ=255)                                                     gACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGGGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGTTCAATACGCGCCGCATGAAGCACGTTCTGTTATTTTTTGTCGTGGAtt                                                                                 >  2:495433/1‑140 (MQ=255)                                                             aGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGc                                                                         <  2:217919/140‑1 (MQ=255)                                                             aGCTGTTTCAGACCGCGCAAATCTTTAAACGTGGTGGCGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGc                                                                         <  1:277419/140‑1 (MQ=255)                                                               cTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGA‑TTGTGTGGCAgg                                                                      <  1:959577/140‑1 (MQ=255)                                                                tGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAgg                                                                      <  1:829996/140‑1 (MQ=255)                                                                            tGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCTTCATGGTGTa                                                          >  1:711109/1‑140 (MQ=255)                                                                            tGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTa                                                          >  2:935100/1‑140 (MQ=255)                                                                            tGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTa                                                          >  2:566845/1‑140 (MQ=255)                                                                            tGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTa                                                          >  1:195706/1‑140 (MQ=255)                                                                            tGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTa                                                          >  1:563564/1‑140 (MQ=255)                                                                            tGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTGTTGACGTGGATTAGTGTTGCAGGCGTCATGGTGTa                                                          >  2:378065/1‑140 (MQ=255)                                                                            tGCAAATCGTCAAAGGGGGAGGTGAACGCGTGGAAGCCACCTTGTACCAGGAAACGCTTTATCCCCAGCTCAATAAGCGCCGCTTCCAGCGCGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGCCGTCATGgtgtt                                                          >  2:184838/1‑139 (MQ=255)                                                                             gCAAATCTTCAAAGGGGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTag                                                         >  1:647933/1‑140 (MQ=255)                                                                              cAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTagc                                                        <  2:6167/140‑1 (MQ=255)                                                                              cAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTagc                                                        <  2:45877/140‑1 (MQ=255)                                                                                aaTCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCTTCATGGTGTagcag                                                      <  2:711109/140‑1 (MQ=255)                                                                                      cAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCg                                                <  2:244206/140‑1 (MQ=255)                                                                                       aaaGGTGGTGGTGAATGCGTGGTAGGCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGt                                               <  1:495433/140‑1 (MQ=255)                                                                                       aaaGGTGGTGGTGAACGCGTGGCAGCCACCTAGTTCCAGAAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGt                                               <  2:332455/140‑1 (MQ=255)                                                                                       aaaGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGt                                               <  2:114847/140‑1 (MQ=255)                                                                                         aGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTAc                                             <  1:871370/140‑1 (MQ=255)                                                                                              gtggtgAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATc                                        >  2:602012/1‑140 (MQ=255)                                                                                                     aCGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGt                                               >  1:1018382/1‑126 (MQ=255)                                                                                                     aCGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGt                                               <  2:1018382/126‑1 (MQ=255)                                                                                                     aCGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAgc                                 >  2:187123/1‑140 (MQ=255)                                                                                                        cgTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGcgcg                              <  1:187123/140‑1 (MQ=255)                                                                                                        cgTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGcgcg                              <  2:917894/140‑1 (MQ=255)                                                                                                         gTGGAAGCCACCTTGTTCCAGGAAACGCTTTCTCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGt                             >  1:714219/1‑140 (MQ=255)                                                                                                         gTGGAAGCCACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGt                             >  2:56501/1‑140 (MQ=255)                                                                                                                 cACCTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCg                     >  2:563611/1‑140 (MQ=255)                                                                                                                   ccTTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTTGCAGCTTGCGTACTCAGCGACAAGCGCGTTAACATCGcc                   >  2:382616/1‑140 (MQ=255)                                                                                                                           cAGGAAACGCTTTATCCCCAGCCCAGTACCCGCCGCTCCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGa           <  1:291486/140‑1 (MQ=255)                                                                                                                             ggAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATg         <  1:62437/140‑1 (MQ=255)                                                                                                                              gAAACGCTTTATCCCCAGCTCAATTCGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTCCTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATgg        <  2:78548/140‑1 (MQ=255)                                                                                                                              gAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATgg        <  1:602012/140‑1 (MQ=255)                                                                                                                              gAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATgg        <  2:812047/140‑1 (MQ=255)                                                                                                                              gAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATgg        >  2:897524/1‑140 (MQ=255)                                                                                                                                    cTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTca  <  2:563564/140‑1 (MQ=255)                                                                                                                                    cTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTca  <  2:195706/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCA  >  NC_000913/67415‑67685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑