Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,407,849	G→A	M81I (ATG→ATA)	panF →	pantothenate:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,407,849	0	G	A	100.0%	114.6 / NA	35	M81I (ATG→ATA)	panF	pantothenate:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/19);  total (16/19)

TTCCTCGGCAGCCGCTCTATGGGCGGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGTCTTCTGGTGTGGCTG  >  NC_000913/3407715‑3407984                                                                                                                                       |                                                                                                                                        ttCCTCGGCAGCCGCTCTATGGGCGGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCa                                                                                                                                    <  2:859430/140‑1 (MQ=255)      cGGCAGCCGCTCTATGGGCGGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTAAATACGGGCTGGGCTGGGGATTTCTGGCGATAATTTAGCttt                                                                                                                               >  1:520436/1‑139 (MQ=255)       ggCAGCCGCTCTATGGGCGGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCCGGGGCTGCTTATAAATACAGGCTGGGGTGGTGAATGCTGGAGATAATTTAGCTTcc                                                                                                                              >  1:500287/1‑140 (MQ=255)                      ggCGGTATTGTGCTGGGGATGACGCTCACCGCGACATATTTCAGTGCCGGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTc                                                                                                               <  2:464575/140‑1 (MQ=255)                          gTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTc                                                                                                           <  2:952831/140‑1 (MQ=255)                                     gCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCCGGAGCTGCTTATAAAAACGGGCTGGGGTGGGGATTGCGGGCGATAACTTAACTTGCCGCAGGCTGGCTCTCACTCCGTATtctccg                                                                                                >  2:362141/1‑138 (MQ=255)                                     gCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTATAAATACGGGCTGGGCTGGGGATTGCGGGCGATAATTCACCTTGCTGCAGTCGGGCTGTCACTCGGTATTCTCgg                                                                                                >  2:299282/1‑140 (MQ=255)                                          gACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCCGCTTATAAAAACGGGCGGGGCTGGGGGTTTCTGGCGGTAATTTAACTTCCTGCAGGCTGGCTTTTACTCCGGATTTTCCGCaaga                                                                                           >  2:204446/1‑140 (MQ=255)                                                                  tGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGGATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGGATTCTCGACAAGAATTTTGCGTTTCTTGCGCGCCGCt                                                                   >  1:275764/1‑140 (MQ=255)                                                                  tGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAAAACCGGCTGGGCTGGGGGTTTCTGGCGATAATTCAGCTTCCTGCCGTCTGGGTTTTACTCAGGATTTTACGCAACAAGTTTGCGGTTCTTGCGcgccgcc                                                                   >  2:350269/1‑139 (MQ=255)                                                                   gCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTa                                                                  <  1:204446/140‑1 (MQ=255)                                                                      aGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACaa                                                               <  1:932444/140‑1 (MQ=255)                                                                      aGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAAAACGGGGTGGGCGGGGGATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCCAGAAAGTTTCGATTCTTTCGCGCCGCCACaa                                                               >  2:939874/1‑140 (MQ=255)                                                                                  ggcggGCCAGGAGCTCCTTATAAATTCGGTCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCt                                                   <  1:450828/140‑1 (MQ=255)                                                                                    cggGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGa                                                 <  1:145003/140‑1 (MQ=255)                                                                                    cggGCCAGGAGCTGCTTATAAACACAGGCAGGGCTGGCTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGa                                                 <  1:406875/140‑1 (MQ=255)                                                                                          aGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGata                                           <  2:65381/140‑1 (MQ=255)                                                                                                 gctTATAAATACGGGCTGGGCTGGGTTTTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGtt                                    >  2:838228/1‑140 (MQ=255)                                                                                                 gctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGtt                                    >  2:672288/1‑140 (MQ=255)                                                                                                  ctTATAAATACGGGCTGGGCTGGGTATTGCTAGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGttt                                   >  1:25638/1‑140 (MQ=255)                                                                                                   ttATAAATCCGGGCTGGCCTGGGTATTGCTGACGACAATTCAGCTTCCCGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTg                                  <  2:480433/140‑1 (MQ=255)                                                                                                   ttATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTg                                  <  1:672288/140‑1 (MQ=255)                                                                                                   ttATAAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTg                                  <  2:87847/140‑1 (MQ=255)                                                                                                     ataAATACGGGCTGGGCCGGGTCTTGCTGGCTATACTTCCGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGcc                                <  2:275764/140‑1 (MQ=255)                                                                                                      taAATACGGGCTGGGCTGGGTATTGCTGGCGATAATTCACCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGccc                               <  2:435076/140‑1 (MQ=255)                                                                                                            cGGGCTGGGCTGGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTAcc                         >  1:863331/1‑140 (MQ=255)                                                                                                              ggctgggctgGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGcc                                >  2:209711/1‑131 (MQ=255)                                                                                                              ggctgggctgGGTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGcc                                <  1:209711/131‑1 (MQ=255)                                                                                                                ctgggctgggTATTGTTGGCGACAAATCACCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCagag                     <  1:290947/140‑1 (MQ=255)                                                                                                                   ggctgggTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCagag                     >  2:846497/1‑137 (MQ=255)                                                                                                                   ggctgggTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCagag                     <  1:846497/137‑1 (MQ=255)                                                                                                                       gggTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGtctt              <  1:939874/140‑1 (MQ=255)                                                                                                                        ggTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGtcttc             >  2:240678/1‑140 (MQ=255)                                                                                                                        ggTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGtcttc             >  2:171904/1‑140 (MQ=255)                                                                                                                         gTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGtcttct            >  1:114264/1‑140 (MQ=255)                                                                                                                         gTATTGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGtcttct            >  1:557008/1‑140 (MQ=255)                                                                                                                             tGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAgtc                   <  2:744536/129‑1 (MQ=255)                                                                                                                             tGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAgtc                   >  1:744536/1‑129 (MQ=255)                                                                                                                             tGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGTCTTCTGgtg        >  2:725089/1‑140 (MQ=255)                                                                                                                             tGCTGGCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGTCTTCTGgtg        >  1:536349/1‑140 (MQ=255)                                                                                                                                  gCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGTCTTCTGGTGtggct   >  2:753920/1‑140 (MQ=255)                                                                                                                                  gCGATAATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGTCTTCTGGTGtggct   >  1:540100/1‑140 (MQ=255)                                                                                                                                   cGATAATTCAGCTTCCTGCAGTATGTCTTGCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGTCTTCTGGTGtggctg  <  1:350269/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTCCTCGGCAGCCGCTCTATGGGCGGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCTACAATGCAGTGACGCTGAACGATATGCTGTTTGCCCGCTACCAGAGTCGTCTTCTGGTGTGGCTG  >  NC_000913/3407715‑3407984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑