Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,437,256 C→T L247L (CTG→TTG)  trkA → NAD‑binding component of Trk potassium transporters

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,2560CT98.4% 194.0 / NA 62L247L (CTG→TTG) trkANAD‑binding component of Trk potassium transporters
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (35/26);  minor base A (1/0);  total (36/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTGAAGCTGGTGATGAAGTGTTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGA  >  NC_000913/3437123‑3437392
                                                                                                                                     |                                                                                                                                        
tgttgAAGCTGGTGATGAAGTGTTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGgcgc                                                                                                                                    >  1:743257/1‑140 (MQ=255)
         tGGTGATGAAGTGTTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTgg                                                                                                                           <  1:627143/140‑1 (MQ=255)
          ggTGATGAAGTGTTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGa                                                                                                                          >  1:177257/1‑140 (MQ=255)
           gTGATGAAGTGTTCTTCATTGCCGCTTCACAGCGTGTCCCCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGaa                                                                                                                         <  2:441239/140‑1 (MQ=255)
                  aGTGTTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGaa                                                                                                                         <  2:452580/133‑1 (MQ=255)
                  aGTGTTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGaa                                                                                                                         >  1:452580/1‑133 (MQ=255)
                  aGTGTTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAAGATCGGTGCAGGTTTGGCGCGTCGTCTGGAAAAAGAtt                                                                                                                  >  1:441239/1‑140 (MQ=255)
                   gtgtTCTTTATTGCCGCATCACAGCATATCCGCGCAGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTa                                                                                                                 <  2:355566/140‑1 (MQ=255)
                     gtTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACa                                                                                                               >  1:750433/1‑140 (MQ=255)
                     gtTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGAACATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCTACGTCTGGaaaaaaaataaa                                                                                                               >  2:1014311/1‑133 (MQ=255)
                      ttCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGaa                                                                                                                         >  1:227331/1‑129 (MQ=255)
                      ttCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGaa                                                                                                                         <  2:227331/129‑1 (MQ=255)
                             ttGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTaaa                                                                                                       <  1:987952/140‑1 (MQ=255)
                              tGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATTTCGGTGCAGGGTGGGCACGTCGTCTAGAAAAAGATTACAGCGTTAAAc                                                                                                      >  2:438027/1‑140 (MQ=255)
                                ccGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATTGGTGCAGGGTTGGCTCGTCGTCTGGGAAAAGTTTACAGCGTTAAACTc                                                                                                    >  2:461984/1‑140 (MQ=255)
                                ccGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTc                                                                                                    >  2:898685/1‑140 (MQ=255)
                                ccGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTGGGCGCGTAGTCTGGGAAAAGAGTACAGCGTTAAACTc                                                                                                    >  1:291408/1‑140 (MQ=255)
                                ccGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATAGGTGCAGGCTTGACGCATCGTCTGGAAAAAGATTACAGCGTTAAACTc                                                                                                    >  2:312053/1‑140 (MQ=255)
                                  gCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCAt                                                                                                  <  1:112845/140‑1 (MQ=255)
                                  gCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCAt                                                                                                  <  1:133267/140‑1 (MQ=255)
                                  gCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCAt                                                                                                  <  1:10538/140‑1 (MQ=255)
                                  gCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGCGCAGGGATGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCAt                                                                                                  >  2:831795/1‑140 (MQ=255)
                                     tCACAGCATATCCGCCCGGTGATGAGTGAGTTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGa                                                                                               <  1:383132/140‑1 (MQ=255)
                                              atCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATca                                                                                      >  1:225117/1‑140 (MQ=255)
                                              atCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATca                                                                                      >  1:985325/1‑140 (MQ=255)
                                                   cgcgGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCagcagc                                                                                 >  2:12354/1‑140 (MQ=255)
                                                       gTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAgcgcgc                                                                             >  2:84007/1‑140 (MQ=255)
                                                        tgatgaGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCt                                                                            >  1:938992/1‑140 (MQ=255)
                                                                           aCTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGt                                                         >  2:226767/1‑140 (MQ=255)
                                                                           aCTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGt                                                         >  1:844806/1‑140 (MQ=255)
                                                                           aCTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTAGCGGAAAAGt                                                         >  1:162740/1‑140 (MQ=255)
                                                                             tGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTa                                                       <  2:225117/140‑1 (MQ=255)
                                                                             tGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTa                                                       <  2:177257/140‑1 (MQ=255)
                                                                             tGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTa                                                       <  1:653/140‑1 (MQ=255)
                                                                             tGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTa                                                       <  1:898685/140‑1 (MQ=255)
                                                                             tGGAAAAACCGTATAAGCGGATCATGCTGCTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTa                                                       <  1:461984/140‑1 (MQ=255)
                                                                                         ataAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATc                                           >  1:202767/1‑140 (MQ=255)
                                                                                         ataAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATc                                           >  2:860669/1‑140 (MQ=255)
                                                                                         ataAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGGAATCAGCAGCGCACTGGCGAACTGGGGGAACAGTTACAGAATACGATc                                           >  2:285594/1‑140 (MQ=255)
                                                                                         ataAGCGGATCATGCGGGTTGGTGGCGGTTATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATc                                           <  1:438027/140‑1 (MQ=255)
                                                                                          taAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCg                                          >  1:791868/1‑140 (MQ=255)
                                                                                            aGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTc                                        <  2:505599/140‑1 (MQ=255)
                                                                                                gATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCtttt                                    >  2:855531/1‑140 (MQ=255)
                                                                                                    aTGCTGGTTGGTGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTgg                                >  1:604545/1‑140 (MQ=255)
                                                                                                        tggttggtGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCCGCCGAACTGGCGGAAACGTTACAGAATACGCTCGTCTTTTTTGGTGAt                            >  2:403147/1‑140 (MQ=255)
                                                                                                         ggttggtGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATg                           >  1:230824/1‑140 (MQ=255)
                                                                                                          gttggtGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGc                          <  2:604545/140‑1 (MQ=255)
                                                                                                           ttggtGGCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCg                         >  2:880713/1‑140 (MQ=255)
                                                                                                            tggtggCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGt                        <  2:844806/140‑1 (MQ=255)
                                                                                                            tggtggCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGt                        <  2:202767/140‑1 (MQ=255)
                                                                                                            tggtggCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGt                        <  1:555650/140‑1 (MQ=255)
                                                                                                            tggtggCGGTAATATAGGTGCACGGATGGGGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGt                        <  1:482690/140‑1 (MQ=255)
                                                                                                                ggCGGTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGa                    <  1:880713/140‑1 (MQ=255)
                                                                                                                   ggTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTCACAGAGTGTGATCGTCCTTTTTGGTGATGCGTCGGATCa                 >  2:482690/1‑140 (MQ=255)
                                                                                                                    gTAATATCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTGCAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCaa                >  2:307448/1‑140 (MQ=255)
                                                                                                                       atatCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGaa             <  2:938992/140‑1 (MQ=255)
                                                                                                                       atatCGGTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTGTGGTCATGCGTCGGATCAAGaa             >  2:299582/1‑140 (MQ=255)
                                                                                                                            ggTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAactact        >  2:71447/1‑140 (MQ=255)
                                                                                                                            ggTGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAactact        <  1:403147/140‑1 (MQ=255)
                                                                                                                              tGCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTgg      <  1:855531/140‑1 (MQ=255)
                                                                                                                               gCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACTGAATACGATCGTGTGTTTTGGTGATCCGTCCCATCAAGAACTACTGGc     >  2:439540/1‑140 (MQ=255)
                                                                                                                               gCAGGGTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGc     >  1:118351/1‑140 (MQ=255)
                                                                                                                                 aGGGTTGGCGCGTCGTCTGGAACAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCg   <  2:291408/140‑1 (MQ=255)
                                                                                                                                  gggTTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCGTTTTTGGGGATACGGCGGATCGAGAACTACTGGCCga  >  1:292083/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                        
TGTTGAAGCTGGTGATGAAGTGTTCTTTATTGCCGCTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAGCGGATCATGCTGGTTGGTGGCGGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGA  >  NC_000913/3437123‑3437392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑