Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,491,988	C→T	G104S (GGC→AGC)	ppiA ←	peptidyl‑prolyl cis‑trans isomerase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,491,988	0	C	T	100.0%	196.6 / NA	57	G104S (GGC→AGC)	ppiA	peptidyl‑prolyl cis‑trans isomerase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/35);  total (22/35)

TACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACA  >  NC_000913/3491854‑3492121                                                                                                                                       |                                                                                                                                       tACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTAt                                                                                                                                   >  2:177378/1‑140 (MQ=255)  aCCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATc                                                                                                                                  >  1:501059/1‑140 (MQ=255)  aCCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATAGTGCCAGGCGTGTGGCGCAGGCTGTTATc                                                                                                                                  >  1:306909/1‑140 (MQ=255)    cgcgTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATc                                                                                                                                  <  2:597597/138‑1 (MQ=255)    cgcgTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATc                                                                                                                                  >  1:597597/1‑138 (MQ=255)    cgcgTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCgg                                                                                                                                >  1:1005019/1‑140 (MQ=255)     gcgTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGc                                                                                                                               <  2:544403/140‑1 (MQ=255)      cgTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCt                                                                                                                              >  1:182461/1‑140 (MQ=255)      cgTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCt                                                                                                                              >  2:26382/1‑140 (MQ=255)      cgTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACACGGGTTGCGAAGGCTGTTATCGGCt                                                                                                                              >  1:414236/1‑140 (MQ=255)       gTAACCGAAATCACGATGACCACGGTCAACGAAGGCGCTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCtt                                                                                                                             <  1:263243/140‑1 (MQ=255)          aCCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTACCATCGCGATGGGGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCAt                                                                                                                          >  2:319525/1‑140 (MQ=255)                         aCCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑Gggt                                                                                                          >  2:31704/1‑140 (MQ=255)                            aTGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑Gggtttg                                                                                                       <  1:971914/140‑1 (MQ=255)                                         gCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGGTTGGC‑GGGTGTTGtttttttttctt                                                                                          >  1:347093/1‑134 (MQ=255)                                         gCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTTGtttttttttgtt                                                                                          >  2:742086/1‑134 (MQ=255)                                         gCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTctgctg                                                                                          <  1:723222/140‑1 (MQ=255)                                          cGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTCTGATTGGC‑GGGTTTTGGTTTTTTttctgc                                                                                         >  1:358892/1‑140 (MQ=255)                                          cGTTATCCGCAACGTTGATAAAGAACTGGCTGATGGCGCTGTCTTTGACAGCGATACGTGCCATCGCGCTGGTGCCACCCGTGTTTCGCAGGCCGTTATCGGCTTCTTTTTTTATTAGC‑GGGGTTGGtttttttttgggg                                                                                         >  2:744841/1‑133 (MQ=255)                                              aTCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCt                                                                                     >  1:779457/1‑140 (MQ=255)                                                cGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGc                                                                                   <  1:549428/140‑1 (MQ=255)                                                 ggCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCt                                                                                  <  1:742086/140‑1 (MQ=255)                                                              aaGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAAccgcc                                                                     <  2:501059/140‑1 (MQ=255)                                                              aaGAACTGGCTGGTGGCACTGTATTTGTCAGCGGTACGTCCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAAccgcc                                                                     <  2:306909/140‑1 (MQ=255)                                                                 aaCTGGCTGGTGGCGTTGTCTTTGTTAGCTGTACGTGACATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCgccgcc                                                                  <  1:319525/140‑1 (MQ=255)                                                                 aaCTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCgccgcc                                                                  <  2:196252/140‑1 (MQ=255)                                                                    tggctggTGGCGCTGTCTTTGTGAGTGGTCCGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTg                                                               <  1:318891/140‑1 (MQ=255)                                                                    tggctggTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTg                                                               <  2:727197/140‑1 (MQ=255)                                                                      gctggTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGCGGGGTTGGGATTTTTTTGTTGC‑TCTTGTCGGAGGAAACCCCGCCCtcga                                                             >  2:400573/1‑137 (MQ=255)                                                                       ctggTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTTGTTTTATTTGCTGGCTCAGCACGGTGAAACCGCCGCCCTGAAt                                                            >  1:339262/1‑140 (MQ=255)                                                                          gtggCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGAGGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCAt                                                         <  1:433871/140‑1 (MQ=255)                                                                                cTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGGTTTTTTTTCTGCATCTGCTCGGTGGAACCCCCCCCCCCAATCATAAAgac                                                   >  2:67285/1‑138 (MQ=255)                                                                                  gTCTTTGTCAGCGGTATCTGCCTTCGCGATGCTTCGACGAGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAg                                                 <  2:464519/140‑1 (MQ=255)                                                                                     tttGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGGTTTTTTTTCTGCATCTGCTCGGGGGAACCGCCGCCCCGAATCATAAAGCCAGGaa                                              >  1:824454/1‑140 (MQ=255)                                                                                        gTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGa                                           <  1:31704/140‑1 (MQ=255)                                                                                         tCAGCGGTACGTGCCATCGCGATGGTGCCACCCGTTTTGTGCAGGCTGCTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAc                                          <  2:386531/140‑1 (MQ=255)                                                                                          cAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcg                                         <  1:983390/140‑1 (MQ=255)                                                                                          cAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcg                                         <  2:779457/140‑1 (MQ=255)                                                                                          cAGCGGTACGTGCCAGCGCGATAGTACCATGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcg                                         <  1:481436/140‑1 (MQ=255)                                                                                           aGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGCGGGGTTTGGTTTTTT‑TTCTGCATCTTCTCGGTGGAAACGCCCCCCCGAATCATAAAACCAAGGAAGAcgc                                        >  2:75458/1‑140 (MQ=255)                                                                                            gCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgcg                                       <  1:177378/140‑1 (MQ=255)                                                                                            gCGGTACGGGCCATCGGGATGGCGCCACGCGTGCTCCGCTGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgcg                                       <  2:458699/140‑1 (MQ=255)                                                                                             cGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCgg                                      <  1:67285/140‑1 (MQ=255)                                                                                             cGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGTTTTTTTTTCTGCATCTTCTCCGTGGAACCCCCCCCCCGAATCATAAAAACAAGAATGACGCgg                                      >  2:861539/1‑140 (MQ=255)                                                                                               gTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTg                                    <  1:981261/140‑1 (MQ=255)                                                                                               acaggTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTg                                    <  1:847567/136‑1 (MQ=255)                                                                                               acaggTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTg                                    <  1:694569/136‑1 (MQ=255)                                                                                                         tCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAgtg                          <  1:209102/140‑1 (MQ=255)                                                                                                         tCGCGATGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAgtg                          <  1:207428/140‑1 (MQ=255)                                                                                                              aTGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGTTTTTTTTTCTTCCTCTGCTCGGTGGAAACGCCGCCCCGAATCATAAAGCCAGGGAAGACGCGGGGGAAGGGAgggtctgt                     >  2:847567/1‑136 (MQ=255)                                                                                                               tGGTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTGTTTTTTTTTCTGCATCTGCTCGGGGGAAACGCCCCCCCGGATCATAAAACCAGGGATGACGCGGGGGAAAGGAGTggtgttt                    >  2:207428/1‑139 (MQ=255)                                                                                                                ggTGCCACGCGTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTT‑‑‑CTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATaaa                <  2:637768/140‑1 (MQ=255)                                                                                                                ggTGCCACGCGTGTTCCGCAGGGTGTTATCGGCTTCAATTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTtat                   <  1:369024/140‑1 (MQ=255)                                                                                                                   gCCACGCGTGTTGCGGAGGCTGCTAGCGGCTTCATTTGTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATaaa                <  1:400573/140‑1 (MQ=255)                                                                                                                        gcgTGTTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCg           <  1:35534/140‑1 (MQ=255)                                                                                                                          gtgtTGCGCAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCt         <  2:182461/140‑1 (MQ=255)                                                                                                                             ttGCGCAGGCTGTTATCGGCTTAATTTTTGATTGGC‑GGGGTTGGTTTTATCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGtt      <  2:358892/140‑1 (MQ=255)                                                                                                                                 gcAGGCTGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTcaca  <  1:49489/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACA  >  NC_000913/3491854‑3492121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑