Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,545,166	T→C	R95R (CGT→CGC)	yhgH →	DNA utilization protein YhgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,545,166	0	T	C	100.0%	97.6 / NA	29	R95R (CGT→CGC)	yhgH	DNA utilization protein YhgH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/20);  total (9/20)

CCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGT  >  NC_000913/3545030‑3545295                                                                                                                                         |                                                                                                                                  ccTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTg                                                                                                                                <  2:880972/140‑1 (MQ=255) ccTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGCGAAATCGCCAGCGCCCTGTCACGCCTg                                                                                                                                >  2:736032/1‑140 (MQ=255)   tGCGGTCGCTGCCTGCACAAACCGCCGCCCTAGCATAGATTGGTCACGATTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGtt                                                                                                                              <  2:293348/140‑1 (MQ=255)   tGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGtt                                                                                                                              <  1:863898/140‑1 (MQ=255)   tGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGtt                                                                                                                              <  1:965117/140‑1 (MQ=255)   tGCGGTCGCGGCCTGCACAAACTGCCGCCCGGGCAGAGATTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGtt                                                                                                                              <  2:359570/140‑1 (MQ=255)   tGCGGCGGCTGCCTGCAGAAACGGCCGCCCTGGCAGAGATTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGtt                                                                                                                              <  2:432759/140‑1 (MQ=255)    gCGGTCGCTGCCTGCGAAAACCCCCCCCCTGGCAAAGCCTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTa                                                                                                                             <  1:318336/140‑1 (MQ=255)           ctgcctgcAAAACCCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTgg                                                                                                                      <  2:192293/140‑1 (MQ=255)              cctgcAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAg                                                                                                                   <  1:963268/140‑1 (MQ=255)                         cgccgcCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCt                                                                                                        <  2:893872/140‑1 (MQ=255)                         cgccgcCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCt                                                                                                        <  1:645866/140‑1 (MQ=255)                            cgccCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCtcgt                                                                                                     >  1:61994/1‑140 (MQ=255)                                   gCAAAGACTGGTCACCGTTGCCTACCATGCGCCGCCGCTTAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGcacca                                                                                              <  1:315745/140‑1 (MQ=255)                                         aCTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTAGCACCACCGGGt                                                                                        <  2:865819/140‑1 (MQ=255)                                           tGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTg                                                                                      >  1:562005/1‑140 (MQ=255)                                             gTCACGGTTGCCGACTATGCGCCGCCGTTTAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACCATTGGAATTCTTACACTCTCGGCGCACCACCGGGTTGCa                                                                                    >  2:315745/1‑140 (MQ=255)                                                              tGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAATCTTACACTCTCTTTGAGCCACCGGATTGCAATTGCCGGCTCGCATCg                                                                   >  2:492093/1‑140 (MQ=255)                                                                     ccgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCTAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGt                                                            <  1:704860/140‑1 (MQ=255)                                                                     ccgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGt                                                            <  1:162896/140‑1 (MQ=255)                                                                        ttAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTcc                                                         >  1:31900/1‑140 (MQ=255)                                                                                             cTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTTGCACCACCGGGTTGCAATTGCCGGTTCGGATCGTCAGCGTTTCGTTATGGCAGCGGCGTCACTg                                    >  2:400032/1‑140 (MQ=255)                                                                                                   ttttCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCTCGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCg                              <  2:277860/140‑1 (MQ=255)                                                                                                             ccAGTGAAATCGCCAGCGCCCTGTCACGGTTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGGGGCGTCACTGGCGTCGGGGATTTAAt                    <  2:937576/139‑1 (MQ=255)                                                                                                                     aTCGCCAGCGCCCTGTCACGCCTGTTACTATTGAATGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGa            <  2:466609/140‑1 (MQ=255)                                                                                                                         ccAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTg        <  2:31900/140‑1 (MQ=255)                                                                                                                          cAGCGCCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGc       >  1:568137/1‑140 (MQ=255)                                                                                                                           aGCGCCCTGTCACGCCTGTTACCATTGGAAGCCTTACACACTCGTCGCTCCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCt      <  2:365996/140‑1 (MQ=255)                                                                                                                             cgcCCTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGACACTGGCATCGAGGATTTAATCAGAGCGATTTGCtgt    >  1:365996/1‑140 (MQ=255)                                                                                                                               cccTGTCACGCCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTgtgt  <  2:562005/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  CCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGT  >  NC_000913/3545030‑3545295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑