Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,545,888	T→C	L89L (TTA→CTA)	nfuA →	iron‑sulfur cluster carrier protein NfuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,545,888	0	T	C	100.0%	271.2 / NA	75	L89L (TTA→CTA)	nfuA	iron‑sulfur cluster carrier protein NfuA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/41);  total (34/41)

TCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCG  >  NC_000913/3545755‑3546016                                                                                                                                      |                                                                                                                                 tCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGc                                                                                                                            >  2:834933/1‑140 (MQ=255)   cgccgGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTg                                                                                                                          >  1:40314/1‑140 (MQ=255)   cgccgGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTg                                                                                                                          >  1:558208/1‑140 (MQ=255)   cgccgGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTg                                                                                                                          >  2:503428/1‑140 (MQ=255)    gccgGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATTCAGAGATCAATTATGTTACCGACCATTTGGGTTCCCAGCTAACGCTGa                                                                                                                         >  2:271883/1‑140 (MQ=255)           gCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGcccc                                                                                                                  <  2:786536/140‑1 (MQ=255)             tgtgGAAGCCACCGACAAAGCCCTGAAATTTAACCGGCTTACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGa                                                                                                                <  2:317797/140‑1 (MQ=255)                    gCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCaa                                                                                                         >  2:252919/1‑140 (MQ=255)                         cGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACTGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGc                                                                                                    >  2:969678/1‑140 (MQ=255)                         cGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGc                                                                                                    >  2:713853/1‑140 (MQ=255)                            cacaGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTa                                                                                                 <  2:184756/140‑1 (MQ=255)                             acaGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTaa                                                                                                <  2:165577/140‑1 (MQ=255)                               aGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAg                                                                                              <  1:103417/140‑1 (MQ=255)                                gCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATACAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGt                                                                                             >  2:11612/1‑140 (MQ=255)                                   cTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGc                                                                                          >  2:217844/1‑140 (MQ=255)                                      aaaTTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCCCCATACCTGGAAGATGCAGAGATCAATTTTGTTACCGACCAGTTGGTTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGa                                                                                       <  2:57833/140‑1 (MQ=255)                                           tGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGTCCCGAACGCCAATATGCCTAAAGTGGCAGACGATg                                                                                  >  2:322342/1‑140 (MQ=255)                                           tGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATg                                                                                  >  2:565748/1‑140 (MQ=255)                                           tGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATg                                                                                  >  1:732892/1‑140 (MQ=255)                                            gACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGc                                                                                 <  2:40314/140‑1 (MQ=255)                                             aCCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCa                                                                                >  2:845996/1‑140 (MQ=255)                                              ccTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCAc                                                                               <  1:834933/140‑1 (MQ=255)                                              ccTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCAc                                                                               <  1:78345/140‑1 (MQ=255)                                                  ctgACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCt                                                                           >  2:33180/1‑140 (MQ=255)                                                        gcgTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGGGTAAAGTGGCAGACGATGCACCGCTGATGGa                                                                     <  2:759651/140‑1 (MQ=255)                                                            aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGAt                                                                        <  2:811742/133‑1 (MQ=255)                                                            aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGAt                                                                        >  1:811742/1‑133 (MQ=255)                                                            aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                                 <  1:565748/140‑1 (MQ=255)                                                            aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                                 <  2:669693/140‑1 (MQ=255)                                                            aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                                 <  1:252919/140‑1 (MQ=255)                                                            aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                                 <  1:113726/140‑1 (MQ=255)                                                            aTGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAACGCGTAAAGTGGGAGGCGATGCTCCGCTGATGGAgcgc                                                                 >  1:337558/1‑140 (MQ=255)                                                            aTGTTGATGAGTTAAGCACACCATACCTGGACGATCCAGCGATCGATTCTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                                 <  2:267445/140‑1 (MQ=255)                                                            aTGTTGATGAGTTAAACGCACCTTATCTGTAAGATGCCGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAgcgc                                                                 <  1:271883/140‑1 (MQ=255)                                                             tGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGcgcg                                                                <  2:985685/140‑1 (MQ=255)                                                             tGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGcgcg                                                                <  1:503428/140‑1 (MQ=255)                                                              gTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGt                                                               <  1:845996/140‑1 (MQ=255)                                                              gTTGATGAGTTAAGCCCACCATCCCCGGACGATGCAGCGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGt                                                               <  1:274307/140‑1 (MQ=255)                                                               ttGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTg                                                              <  2:101500/140‑1 (MQ=255)                                                               ttGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTg                                                              >  2:201826/1‑140 (MQ=255)                                                                tgatgaGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTgg                                                             <  1:598321/140‑1 (MQ=255)                                                                tgatgaGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTgg                                                             <  2:544411/140‑1 (MQ=255)                                                                 gatgaGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGa                                                            <  1:33180/140‑1 (MQ=255)                                                                 gatgaGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGa                                                            <  1:902619/140‑1 (MQ=255)                                                                        tAAGCGCACCATACCCGGAAGAGGCAGAGCTCGATTTAGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtg                                                     <  1:322342/140‑1 (MQ=255)                                                                         aaGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtgc                                                    >  2:205431/1‑140 (MQ=255)                                                                           gcgcACCATACCTGGTAGATGCGGATATCTATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtgctg                                                  <  1:287345/140‑1 (MQ=255)                                                                           gcgcACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATAtgctg                                                  <  2:21190/140‑1 (MQ=255)                                                                               aCCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGta                                                         >  1:698699/1‑129 (MQ=255)                                                                               aCCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGta                                                         <  2:698699/129‑1 (MQ=255)                                                                                 cATACCTGGAAGATGTAGAGATCGACTTTTTTACCGATCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCg                                            <  1:316421/140‑1 (MQ=255)                                                                                 cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGTTGGGGCTCGTGGAGTATATGCTGCAGTCg                                            >  1:420565/1‑140 (MQ=255)                                                                                 cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCg                                            <  1:739181/140‑1 (MQ=255)                                                                                 cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCg                                            <  1:217844/140‑1 (MQ=255)                                                                                   tACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCa                                          <  2:732892/140‑1 (MQ=255)                                                                                         gAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGGGTGGAGGATATGCTGCAGTCGCAGATCaa                                    >  1:319578/1‑140 (MQ=255)                                                                                            gATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAAccc                                 >  2:827009/1‑140 (MQ=255)                                                                                             ttGCAGAGATCGATTTTGTTACCGACCAGTGGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGGATATGCTGCAGTCGCAGATCAACCca                                >  2:232173/2‑140 (MQ=255)                                                                                              tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCAGCGCTGATGAAGCGCATAGAGTATATTCTGCATTCGCAGAGCAACCcac                               >  2:499297/1‑140 (MQ=255)                                                                                              tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCcac                               >  2:711877/1‑140 (MQ=255)                                                                                               gCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGGTGCAGTCGCAGATCAACCcaca                              >  1:390879/1‑140 (MQ=255)                                                                                               gCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCcaca                              >  1:232799/1‑140 (MQ=255)                                                                                               gCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCcaca                              >  1:832437/1‑140 (MQ=255)                                                                                               gCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCcaca                              >  2:30697/1‑140 (MQ=255)                                                                                                cAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGATCGCTTGGTGTATATGCTGCAGGCGCAGATCAACCCACAg                             >  2:374926/1‑140 (MQ=255)                                                                                                    gaTCGATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTg                         <  2:517437/140‑1 (MQ=255)                                                                                                       cGATTTTGTTACCGAGCAGTTGGGTTCCCGGCTAACGTTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTg                      <  1:407633/140‑1 (MQ=255)                                                                                                        gATTTTGTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGGATATGCTGCAGGCGCAGATCAACCCACAGCTTGCTgg                     >  1:319342/1‑140 (MQ=255)                                                                                                              gTTACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACgg               <  1:827009/140‑1 (MQ=255)                                                                                                               ttACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACggt              >  1:20977/1‑140 (MQ=255)                                                                                                               ttACCGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACggt              >  1:828127/1‑140 (MQ=255)                                                                                                                  ccGACCAGTTGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACggtggt           <  1:713853/140‑1 (MQ=255)                                                                                                                  ccGACCAGTTGGGTTACCAGCTTACGCTGAAAGCCCCTAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACggtggt           <  1:374926/140‑1 (MQ=255)                                                                                                                          ttGGGTTCCCAGCTATCGCTGACAGTCCCTAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTTGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTc   <  1:438939/140‑1 (MQ=255)                                                                                                                           tGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCg  <  1:201826/140‑1 (MQ=255)                                                                                                                           tGGGTTCCCAGCTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCg  <  1:11612/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 TCCGCCGGACGCTGTGGAAGCCACCGACACAGCCCTGAAATTTGACCTGCTGACCGCGTATGTTGATGAGTTAAGCGCACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCG  >  NC_000913/3545755‑3546016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑