| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,550,178 | T→C | T766A (ACT→GCT) | malP ← | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,550,178 | 0 | T | C | 98.2% | 194.7 / ‑4.4 | 58 | T766A (ACT→GCT) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (29/27); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
CATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCA > NC_000913/3550046‑3550308 | cATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGcc > 2:796721/1‑140 (MQ=255) cAGACGTTTGCTTTCTATCGAGGTTACTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGt < 1:496619/140‑1 (MQ=255) gACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTcg < 2:1007683/140‑1 (MQ=255) gACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTcg < 1:764365/140‑1 (MQ=255) gACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTcg > 2:67772/1‑140 (MQ=255) aCGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTcgc > 2:724809/1‑140 (MQ=255) ttGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTa < 2:800425/140‑1 (MQ=255) tGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTAc > 1:910962/1‑140 (MQ=255) tGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTAc > 2:256561/1‑140 (MQ=255) tGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTAc > 1:13602/1‑140 (MQ=255) ccTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGACGCGCGAGGCCAGGCCACCTGGTCGGGGTACAGCACATCCACCTGCtt > 2:332418/1‑140 (MQ=255) tAGCGTTTTGCCTGCCAGATACTAGCCTGATAATCACGATTAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTtg < 2:389294/140‑1 (MQ=255) tAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTtg < 1:1397/140‑1 (MQ=255) cGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGTGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGc < 1:256561/140‑1 (MQ=255) gTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGcc < 2:157257/140‑1 (MQ=255) ttGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTtg < 2:633133/133‑1 (MQ=255) ttGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTtg > 1:633133/1‑133 (MQ=255) ttGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCctct > 2:765083/1‑140 (MQ=255) ttGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCctct > 2:14723/1‑140 (MQ=255) ttGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTAGTCGCGGAACAGCACATCCACCTGCTTTTGTGCctct > 1:393197/1‑140 (MQ=255) gcctgccAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTAc < 1:796721/140‑1 (MQ=255) aGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAAt < 2:120634/140‑1 (MQ=255) aGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAAt < 1:116766/140‑1 (MQ=255) aTAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCa > 2:683209/1‑140 (MQ=255) aaTCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATc < 1:67772/140‑1 (MQ=255) aTCGCGAATAGAGCGTTCCGAGATACACATACCGCAGGGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCa < 1:332418/140‑1 (MQ=255) cgcgAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCAcc < 1:128778/140‑1 (MQ=255) cgAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTAGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAg > 1:62766/1‑140 (MQ=255) cgAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGG‑‑‑CCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATa > 1:156221/1‑140 (MQ=255) aaTAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGAt > 2:869455/1‑140 (MQ=255) aTAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCATCTGCTTTTTTGCGTGTGCATAAGCTCCGAAACCCGCCATTACCAGATa > 2:490834/1‑140 (MQ=255) agCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGa > 2:815649/1‑140 (MQ=255) agCGATCCGAGCTAAACATACCACATCGGCCGGTATTCAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGa < 1:340716/140‑1 (MQ=255) gATCCGAGCTAAACACACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTGTGTGCCTCGACAGAGGGTGCGAAATACGCCATCACCAGATACGGATcc > 2:258198/1‑139 (MQ=255) aTCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATcgc > 1:751595/1‑140 (MQ=255) cTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTg < 2:154534/140‑1 (MQ=255) cATACCGCAGCGGGCGGTATTGTGGATCGCCTCGCTAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGc < 2:393197/140‑1 (MQ=255) cATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGc < 1:683209/140‑1 (MQ=255) tACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCg < 2:813006/140‑1 (MQ=255) aCCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGa > 1:734643/1‑140 (MQ=255) cGCAGCGGGTGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATa > 1:765641/1‑140 (MQ=255) cGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATa < 1:765083/140‑1 (MQ=255) cGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATa < 1:815649/140‑1 (MQ=255) aGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATcgccg > 2:946633/1‑122 (MQ=255) aGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATcgccg < 1:946633/122‑1 (MQ=255) aGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACtg > 1:564133/1‑140 (MQ=255) aGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACtg > 1:544594/1‑140 (MQ=255) aGCGGGCGGTATTGAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACtg > 1:67223/1‑140 (MQ=255) gAGGATCGCCGCGCGCGCCCAGCCCTCCTAGTCGCGGCACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGt < 1:258198/140‑1 (MQ=255) gAGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGt < 2:563377/140‑1 (MQ=255) aGGATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTc > 2:251986/1‑140 (MQ=255) gATCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGa > 2:53111/1‑140 (MQ=255) aTCGCCGCGCGAGCCCAGG‑‑‑CCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGc < 2:156221/140‑1 (MQ=255) tCGCCGCGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAg < 1:251986/140‑1 (MQ=255) cgccgcGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAgg > 2:575101/1‑140 (MQ=255) cgccgcGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAgg > 2:695912/1‑140 (MQ=255) ccgcGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCa < 1:204842/140‑1 (MQ=255) ccgcGCGAGCCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCa > 2:204842/1‑140 (MQ=255) | CATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACCTGCTTTTGTGCCTCTACATAGGCTGCGAAATCCGCCATCACCAGATACGGATCGCCGCCCTGTTTGCCGATACTGTGCAGCATCTGGTCGAAGGCA > NC_000913/3550046‑3550308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |