Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,697,872 T→C V472A (GTG→GCG)  bcsE → c‑di‑GMP‑binding protein BcsE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,697,8720TC100.0% 156.2 / NA 44V472A (GTG→GCG) bcsEc‑di‑GMP‑binding protein BcsE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/21);  total (23/21)

TTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATG  >  NC_000913/3697746‑3698006
                                                                                                                              |                                                                                                                                      
ttCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGCTGATCAATTCAGTGGCGAGCTGGCGCAGATGCgcttg                                                                                                                           >  1:329477/1‑140 (MQ=255)
  ctctcATTCTGTCGGCTTAACGATCAGGACACCGCGTGGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCgcttgct                                                                                                                         <  1:389517/140‑1 (MQ=255)
  ctctcATTCTGTCGGATTAACGTTCTGGATACCGCTTTGGATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCgcttgct                                                                                                                         <  2:409870/140‑1 (MQ=255)
  ctctcATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCgcttgct                                                                                                                         <  2:663198/140‑1 (MQ=255)
    ctcATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCATTGCTGATCTGGCGTAGATGCGcttgcttg                                                                                                                       >  1:478515/1‑140 (MQ=255)
    ctcATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGTCGAGCTGGCGCGGATGCGcttgcttg                                                                                                                       <  1:674019/140‑1 (MQ=255)
    ctcATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCgcttgct                                                                                                                         >  2:663140/1‑138 (MQ=255)
    ctcATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCgcttgct                                                                                                                         <  1:663140/138‑1 (MQ=255)
         tCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCa                                                                                                                  >  2:717452/1‑140 (MQ=255)
          cTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAg                                                                                                                 <  1:781772/140‑1 (MQ=255)
              aggATTAACGATCTGGACACCGAGTTGAATCGTATGTTCTCATTGCCTCCTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACa                                                                                                             <  2:362769/139‑1 (MQ=255)
                           tGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCg                                                                                                <  1:765025/140‑1 (MQ=255)
                           tGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCATGCATGGCCCAGAACAATGGGGCATGcct                                                                                                >  1:362769/1‑139 (MQ=255)
                                 ccGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCt                                                                                          >  2:803668/1‑140 (MQ=255)
                                  cgcgTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCtt                                                                                         >  2:859667/1‑140 (MQ=255)
                                  cgcgTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGATTGCTTGGCCCAGAACAATGGCGCATGCCGCTGCCtt                                                                                         >  1:404699/1‑140 (MQ=255)
                                    cgTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTa                                                                                       >  2:727025/1‑140 (MQ=255)
                                       tGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACg                                                                                    >  2:66258/1‑140 (MQ=255)
                                       tGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCAAGAACAATGGGGCATGCCGCTGCCTTTAACg                                                                                    >  2:334175/1‑140 (MQ=255)
                                         aaTCATATTTTCCCATTTCCAAATGGCGACACTTTCTCAGAACGTATGTTCTGGTTTTAATATGATCTAATCAGTGAAGAGCGGGTGTAGATGCGCTTGCTTGCCCCAGAACAATGGGGCTTGCCGCTGACTTGACCGCa                                                                                  <  2:376848/140‑1 (MQ=255)
                                         aaTCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTACCCCAGAACAAAGGAGCATGCGGCTGCCTTTAACGCa                                                                                  >  1:309182/1‑140 (MQ=255)
                                         aaTCATATTTTCCCATTCCCTACTGGCGACAATTTCTCATACCGTATGATCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCa                                                                                  <  2:309182/140‑1 (MQ=255)
                                                   tCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTaaa                                                                        >  1:729271/1‑140 (MQ=255)
                                                      cATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCg                                                                     <  1:22604/140‑1 (MQ=255)
                                                               cTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAAcc                                                                      >  2:741666/1‑130 (MQ=255)
                                                               cTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAAcc                                                                      <  1:741666/130‑1 (MQ=255)
                                                                     aCATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGcc                                                         >  2:257064/1‑137 (MQ=255)
                                                                     aCATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGcc                                                         <  1:257064/137‑1 (MQ=255)
                                                                         tttCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACg                                                  >  2:963736/1‑140 (MQ=255)
                                                                                 aCCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCATTAACGCAAAGTCCTAAACCGGTCACCAATGCCGAGCACGATGGTCGc                                          >  2:421961/1‑140 (MQ=255)
                                                                                     tATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTGCTAAACCGGTCATCAAGGCCGAGCACGATGGTCGCCACt                                      >  2:309502/1‑140 (MQ=255)
                                                                                             ggTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcgacga                              >  2:227653/1‑140 (MQ=255)
                                                                                             ggTTTGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGcgacga                              >  2:1017722/1‑140 (MQ=255)
                                                                                               tttGAAGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAAt                            >  2:783135/1‑140 (MQ=255)
                                                                                                   aggatgatCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAGCCGGTCATCAATCCCGCGCACGATGCTCGCCACTGGCGACGACTACCa                        >  2:338139/3‑140 (MQ=255)
                                                                                                   aaGATGATCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCa                        >  2:102826/1‑140 (MQ=255)
                                                                                                       tgatCAAATCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAAc                    <  1:227653/140‑1 (MQ=255)
                                                                                                            aaaTCAGTGCCGAGCTGTCGCATATGCGCTTCCTTTCCCCAGAACAATAGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATg               <  2:329477/140‑1 (MQ=255)
                                                                                                             aaTCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGc              <  1:859667/140‑1 (MQ=255)
                                                                                                               tCAGTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGa            <  1:1017722/140‑1 (MQ=255)
                                                                                                                  gTGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCTTCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTg         <  2:796669/140‑1 (MQ=255)
                                                                                                                   tGCCGAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGt        >  2:686065/1‑140 (MQ=255)
                                                                                                                       gAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAga    <  1:968746/140‑1 (MQ=255)
                                                                                                                       gAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAga    <  2:502562/140‑1 (MQ=255)
                                                                                                                       gAGCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAga    <  2:846471/140‑1 (MQ=255)
                                                                                                                       gAGCTGGCACAGATGCCATTGCGTGCCCCATTATAGTGGCGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAga    <  2:478515/140‑1 (MQ=255)
                                                                                                                         gCTGGCGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAgatg  <  1:717452/140‑1 (MQ=255)
                                                                                                                              |                                                                                                                                      
TTCTCTCATTCTGTCGGATTAACGATCTGGATACCGCGTTGAATCATATTTTCCCATTGCCTACTGGCGACATTTTCTCAAACCGTATGGTCTGGTTTGAAGATGATCAAATCAGTGCCGAGCTGGTGCAGATGCGCTTGCTTGCCCCAGAACAATGGGGCATGCCGCTGCCTTTAACGCAAAGTTCTAAACCGGTCATCAATGCCGAGCACGATGGTCGCCACTGGCGACGAATACCAGAACCCATGCGACTGTTAGATG  >  NC_000913/3697746‑3698006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑