Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,249	T→C	Q624R (CAG→CGG)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,249	0	T	C	98.8%	290.1 / NA	82	Q624R (CAG→CGG)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (43/38);  minor base A (0/1);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACG  >  NC_000913/3878115‑3878384                                                                                                                                       |                                                                                                                                        gggTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTAAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGTGGGTAACGGTCCGCtca                                                                                                                                    >  2:777130/1‑140 (MQ=255)  ggTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAGACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCtcat                                                                                                                                   >  2:508612/1‑140 (MQ=255)  ggTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCGCTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCtcat                                                                                                                                   >  2:126279/1‑140 (MQ=255)  ggTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCtcat                                                                                                                                   >  1:647978/1‑140 (MQ=255)      cgcacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCGCCCAGCGGGTAACGGTCCGCTCATCAGa                                                                                                                               <  1:846568/140‑1 (MQ=255)       gcacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGaa                                                                                                                              >  1:739952/1‑140 (MQ=255)        cacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGaaa                                                                                                                             >  1:712001/1‑140 (MQ=255)            cgAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTc                                                                                                                         >  2:558872/1‑140 (MQ=255)            cgAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTc                                                                                                                         >  1:67716/1‑140 (MQ=255)            cgAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTc                                                                                                                         >  1:618975/1‑140 (MQ=255)            cgAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGGAACGGTCCGCTCATCAGAAAGGTc                                                                                                                         >  2:995787/1‑140 (MQ=255)                cAATCGGCTCGAACAGGTTTTGGTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCt                                                                                                                     <  1:457197/140‑1 (MQ=255)                cAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCt                                                                                                                     <  1:49812/140‑1 (MQ=255)                       cTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCa                                                                                                              >  1:861611/1‑140 (MQ=255)                         cGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAAc                                                                                                            >  2:901144/1‑140 (MQ=255)                         cGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAAc                                                                                                            >  2:812547/1‑140 (MQ=255)                            aCAGGTTTTGCTCAGTATTGGTTTGAACACCAAACTTGCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTc                                                                                                         <  1:401915/140‑1 (MQ=255)                                gTTTTGCTCAGCATTGTGGTGAGCATCAAACTTGCGCTGGCTGCCGTGGTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGGTTCCGTCAACGTCGGCt                                                                                                     <  2:443508/140‑1 (MQ=255)                                     gCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatag                                                                                                >  1:790868/1‑140 (MQ=255)                                      cTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgataga                                                                                               >  2:196910/1‑140 (MQ=255)                                         aGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATaa                                                                                            <  1:685106/140‑1 (MQ=255)                                         aGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATaa                                                                                            <  2:176561/140‑1 (MQ=255)                                          gCATTGGTGTGAACATCAAACTTCCGCTGCCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAg                                                                                           <  1:350796/140‑1 (MQ=255)                                           cATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGc                                                                                          <  2:102444/140‑1 (MQ=255)                                               ggTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCtt                                                                                      <  1:156362/140‑1 (MQ=255)                                                gtgtGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgataga                                                                                               <  2:995583/130‑1 (MQ=255)                                                gtgtGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgataga                                                                                               >  1:995583/1‑130 (MQ=255)                                                gtgtGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCttt                                                                                     >  2:46839/1‑140 (MQ=255)                                                 tgtgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTc                                                                                    <  1:184778/140‑1 (MQ=255)                                                   tgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTAAACGTCGGCTGATAGATAAGCTCTTTCAg                                                                                  >  1:464264/1‑140 (MQ=255)                                                     aaCATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCATAAAGGTCAACTTCCGTCAACGTCGGCTGATCGATAAGCTCTTTCAGCa                                                                                >  2:466827/1‑140 (MQ=255)                                                     aaCATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCa                                                                                >  2:723734/1‑140 (MQ=255)                                                     aaCATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCa                                                                                >  2:624482/1‑140 (MQ=255)                                                     aaCATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCa                                                                                >  1:989474/1‑140 (MQ=255)                                                         tCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGc                                                                            <  2:109440/140‑1 (MQ=255)                                                          cAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCTGTCGACGCCGGCTGATTGATAAGCTCTTTCAGCATTGCt                                                                           >  2:375191/1‑140 (MQ=255)                                                          cAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCt                                                                           >  2:682003/1‑140 (MQ=255)                                                          cAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCt                                                                           >  2:644772/1‑140 (MQ=255)                                                          cAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTCCACCCAGTGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCt                                                                           >  1:148283/1‑140 (MQ=255)                                                                     tGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacg                                                                <  2:561429/140‑1 (MQ=255)                                                                        cTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacgacg                                                             <  2:618975/140‑1 (MQ=255)                                                                        cTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacgacg                                                             <  2:67716/140‑1 (MQ=255)                                                                        cTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacgacg                                                             <  2:247982/140‑1 (MQ=255)                                                                        cTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacgacg                                                             <  2:861611/140‑1 (MQ=255)                                                                        cTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCGGACTAGCCCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacgacg                                                             <  2:363088/140‑1 (MQ=255)                                                                         tGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGc                                                            >  1:71917/1‑140 (MQ=255)                                                                           ccGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCGTTCAGCATTGCTTGCGGATAACGACGCTc                                                          >  1:291604/1‑140 (MQ=255)                                                                             gTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCa                                                        <  2:894511/140‑1 (MQ=255)                                                                             gTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCa                                                        <  2:790868/140‑1 (MQ=255)                                                                                 tGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGTGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACg                                                    <  1:777130/140‑1 (MQ=255)                                                                                   ttCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGAt                                                  >  1:23267/1‑140 (MQ=255)                                                                                   ttCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGAt                                                  <  1:644772/140‑1 (MQ=255)                                                                                   ttCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGAt                                                  >  2:960355/1‑140 (MQ=255)                                                                                   ttCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGAt                                                  >  2:756250/1‑140 (MQ=255)                                                                                        tGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCGACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATc                                             >  1:87768/1‑140 (MQ=255)                                                                                        tGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATGAGCTCTTTTAGCATTGGTTTCGGATTACGACGCTCCATACGATTGATc                                             >  2:277892/1‑140 (MQ=255)                                                                                             ttCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGGTAAGGAGGCTCCATACGATTGATCAtttt                                        >  1:257706/1‑140 (MQ=255)                                                                                             ttCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCAtttt                                        >  1:583729/1‑140 (MQ=255)                                                                                             ttCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCAtttt                                        >  2:628525/1‑140 (MQ=255)                                                                                               cAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCt                                      >  2:515039/1‑140 (MQ=255)                                                                                               cAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCt                                      <  1:995787/140‑1 (MQ=255)                                                                                               cAGTTCGCTGACCAGCGCGTTAACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCt                                      >  2:525478/1‑140 (MQ=255)                                                                                                  ttCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGTTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCg                                   <  1:196910/140‑1 (MQ=255)                                                                                                   tCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGt                                  <  1:682003/140‑1 (MQ=255)                                                                                                    cGCTGACCCGCGCGTTATCCCACCGGGTAATGGTCAGCTTATCAGAAACGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTc                                 <  2:494518/140‑1 (MQ=255)                                                                                                    cGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTc                                 <  1:628525/140‑1 (MQ=255)                                                                                                       tGACCAGCGCGTTCATCCAGCGTGTCACGATCCGCTCCTCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTcgcg                              <  1:375191/140‑1 (MQ=255)                                                                                                       tGACCAGCGCGTTCATCCAGCGCGTAACGCTCCGCTCCTCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTcgcg                              <  1:277892/140‑1 (MQ=255)                                                                                                       tGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCGACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTcgcg                              <  2:87768/140‑1 (MQ=255)                                                                                                       tGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTcgcg                              <  2:573110/140‑1 (MQ=255)                                                                                                       tGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTcgcg                              <  1:558872/140‑1 (MQ=255)                                                                                                       tGACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTcgcg                              <  2:739952/140‑1 (MQ=255)                                                                                                       tGACCAGCGCGTTAACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTcgcg                              <  1:525478/140‑1 (MQ=255)                                                                                                        gACCAGCGCGTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGt                             <  2:712001/140‑1 (MQ=255)                                                                                                              cgcgTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACt                       >  2:524007/1‑140 (MQ=255)                                                                                                              cgcgTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACt                       >  2:26957/1‑140 (MQ=255)                                                                                                              cgcgTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACt                       <  2:185125/140‑1 (MQ=255)                                                                                                              cgcgTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACt                       >  2:932528/1‑140 (MQ=255)                                                                                                               gcgTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTc                      <  1:210952/140‑1 (MQ=255)                                                                                                                 gTTCACCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAg                    <  1:812547/140‑1 (MQ=255)                                                                                                                     aCCCAGCGGGTAACGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATAATTGTCAGCGTCGGGTTGTACTCAGATAc                >  1:292394/1‑140 (MQ=255)                                                                                                                                 aCGGTCCGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTaa    >  1:979712/1‑140 (MQ=255)                                                                                                                                   ggTCCGCTCATCAGAAACGTCACCTTCCGTCTACGTCGGCTGATAGATGAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACg  <  2:257706/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACG  >  NC_000913/3878115‑3878384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑