Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,894,503	T→C	V208A (GTC→GCC)	yieE →	putative phosphopantetheinyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,894,503	0	T	C	100.0%	148.5 / NA	42	V208A (GTC→GCC)	yieE	putative phosphopantetheinyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/18);  total (24/18)

TCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAA  >  NC_000913/3894375‑3894637                                                                                                                                 |                                                                                                                                       tCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAg                                                                                                                             <  1:747439/140‑1 (MQ=255)   tGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCAGTGGCGGTAACGACCAAGATTGAAACGCTCGGTGCCTGGGAGTTAGAt                                                                                                                           >  2:299779/1‑140 (MQ=255)          gctgcCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAac                                                                                                                    <  1:709878/140‑1 (MQ=255)           ctgcCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAaca                                                                                                                   >  2:762099/1‑140 (MQ=255)           ctgcCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAaca                                                                                                                   >  2:195349/1‑140 (MQ=255)           ctgcCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAaca                                                                                                                   >  2:194375/1‑140 (MQ=255)                          cGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCt                                                                                                    <  1:177337/140‑1 (MQ=255)                            ccTGAAATGTGCTCATGCAAATTATGTAGAAACGTAATGTGACGCGGATGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGc                                                                                                  <  2:305968/140‑1 (MQ=255)                             cTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGcc                                                                                                 >  2:227320/1‑140 (MQ=255)                               gAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCgg                                                                                               <  2:799596/140‑1 (MQ=255)                                            gTAAATCATGTAGAATCGTTATGCGTCGCTGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCcg                                                                                  <  1:493162/140‑1 (MQ=255)                                               aaTCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCgcgc                                                                               >  2:797214/1‑140 (MQ=255)                                               aaTCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCgcgc                                                                               >  2:1003646/1‑140 (MQ=255)                                                    tGTAGAAGCGTTATGCTACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGGTGGAAAAGCCTACCAGATATTCACAGCCGCGCcaaca                                                                          >  1:302158/1‑140 (MQ=255)                                                    tGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCcaaca                                                                          >  1:124750/1‑140 (MQ=255)                                                    tGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCcaaca                                                                          >  1:648948/1‑140 (MQ=255)                                                    tGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACAGCTGGAACAGCCTGCCGGATAATCACAGCCGCGCcaaca                                                                          >  2:370733/1‑140 (MQ=255)                                                      tAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGATGGCAAACCCTGCCGGCTATTGACAGCCGCGCCAACAAt                                                                        >  2:307741/1‑140 (MQ=255)                                                         aaGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGcc                                                                              <  1:448674/131‑1 (MQ=255)                                                         aaGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGcc                                                                              >  2:448674/1‑131 (MQ=255)                                                          aGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTa                                                                    <  1:194375/140‑1 (MQ=255)                                                              ttATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAg                                                                >  1:503351/1‑140 (MQ=255)                                                              ttATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAg                                                                >  1:719615/1‑140 (MQ=255)                                                                      cgcgGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGgatga                                                        >  2:841943/1‑140 (MQ=255)                                                                        cgGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGatgatg                                                      >  1:538368/1‑140 (MQ=255)                                                                          gAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCg                                                    <  1:971985/140‑1 (MQ=255)                                                                           aaGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGTATATTCACAGCCGCCCCAACAATCCTACCAGCCGGATGATGCGt                                                   >  1:194129/1‑140 (MQ=255)                                                                                gTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGTTATTCACAACCGAGCCCACAATCCTACCAGGCGGATGATGCGTTTTGc                                              >  2:447204/1‑140 (MQ=255)                                                                                 tGCTGGTGTGGTCCGTGGCGGTCACTCCCTCGATTGATAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGcc                                             <  1:306318/140‑1 (MQ=255)                                                                                 tGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGcc                                             <  1:998003/140‑1 (MQ=255)                                                                                   cTGGTGTGGTCCGTGGCGGTCAGGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCa                                           <  1:447204/140‑1 (MQ=255)                                                                                      gtgtGGTCCGTGGCGATCACGCCCACGATGGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAAct                                        <  1:291435/140‑1 (MQ=255)                                                                                            tCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATACTACCAGACGGATGGTGCGTTATGCCCAACTCTCTAc                                  >  1:324998/1‑140 (MQ=255)                                                                                                tGGCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTg                              >  1:934323/1‑140 (MQ=255)                                                                                                 ggCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGa                             >  1:5864/1‑140 (MQ=255)                                                                                                 ggCGGTCACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGGGa                             >  1:928572/1‑140 (MQ=255)                                                                                                       cACGCCCACGATTGAAAAGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCtt                       <  2:934323/140‑1 (MQ=255)                                                                                                               cGATTGAAAAGCTCAGTGCCTGGGAGTTATATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCa               <  1:459396/140‑1 (MQ=255)                                                                                                                        aGCTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAg      <  1:529989/140‑1 (MQ=255)                                                                                                                          cTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGAc    <  2:124750/140‑1 (MQ=255)                                                                                                                          cTCAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGAc    <  1:1003646/140‑1 (MQ=255)                                                                                                                            cAGTGCCTGGGAGTTAGATGGCAAACACGGCTGGAAGAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTTTCTACCGTGAACGCCTTTTCGCCAAATTGATAGACaa  >  1:452694/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                       TCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAA  >  NC_000913/3894375‑3894637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑